29-Jan-2016 Edward Lee "Computational approaches for immune cell receptor repertoire analysis"

29-Jan-2016 Edward Lee "Computational approaches for immune cell receptor repertoire analysis"

Issued: Tue, 16 Feb 2016 16:46:00 GMT

Friday, 29th January 12 pm
Graham Kerr Building, Library

Edward Lee will be speaking on "Computational approaches for immune cell receptor repertoire analysis" on Friday 29th of January at 12 pm in the GK library

"Detailed characterisation of T cell receptor (TCR) diversity involved in immune responses has huge potential benefit for the design of vaccines and immunotherapies. The TCR is heterodimer of two chains (TCRα and TCRβ), and obtaining the paired sequences of these regions is an emerging standard in defining the TCR. Thus, techniques in efficiently sequencing both chains are necessary to measure diversity in T cell responses. A brute force approach utilizes high-throughput sequencing of single cells to obtain the unique TCRα/TCRβ pairs in a sample of T cells, but currently this process remains costly and risks missing small clones. We present an algorithm for efficiently pairing TCRα and TCRβ chains from bulk sequencing of large samples of antigen-specific T cells and estimating relative clonal abundances. We explore the efficiency of these algorithms using artificial datasets.”