Professor Mark Roberts

Professor of Molecular Bacteriology (Veterinary Science & Education )

telephone: 01413305780
email: Mark.Roberts@glasgow.ac.uk

R203C Level 2, Veterinary Biosciences, MacRobert Bldg, Glasgow G61 1QH

Research interests

Salmonella sp. causes a spectrum of diseases in humans and animals ranging from self-limiting enteritis to severe systemic infections. The most well studied salmonella serotype is Salmonella enterica serovar Typhimurium (S. Typhimurium). The cell envelope of Gram negative bacteria such as S. Typhimurium directly interfaces with their host and many eucaryotic antimicrobial defences are targeted at the cell envelope. The cell envelope is protected and repaired by envelope stress responses (ESR).

At least ESR four stress response systems responding to have been identified in E. coli and S. Typhimurium (Nat Rev Micro 4, 383-394 2006)., the most well studied are those controlled by the alternative sigma factor, sigma factor E (sE ), and the two component regulator CpxA/CpxR (Cpx).

We have shown that both of these regulators, and genes controlled by them, are involved in S. Typhimurium pathogenesis and response to various stresses (Infect. Immun. 67, 1560-1568 1999; Microbiology 148, 113-122 2002; Infect. Immun. 71, 5386-5388 2003; Infect Immun 72, 7357-7359 2004; Infect Immun 73, 459-463 2005; Microbiology 153, 263-269 2007).

We are using a variety of methods, including microarrays and proteomics, to identify s E and Cpx regulated genes and study their role in salmonella pathogenesis and defence against various stresses.

Research groups

Publications

List by: Type | Date

Jump to: 2019 | 2017 | 2013 | 2011 | 2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002 | 2001

Number of items: 36.

2019

Bain, M. M. , Zheng, J., Zigler, M., Whenham, N., Quinlan-Pluck, F., Jones, A. C., Roberts, M., Icken, W., Olori, V. E. and Dunn, I. C. (2019) Cuticle deposition improves the biosecurity of eggs through the laying cycle and can be measured on hatching eggs without compromising embryonic development. Poultry Science, 98(4), pp. 1775-1784. (doi: 10.3382/ps/pey528) (PMID:30476345)

Page, A. P. , Roberts, M., Felix, M.-A., Pickard, D., Page, A. and Weir, W. (2019) The golden death bacillus Chryseobacterium nematophagum is a novel matrix digesting pathogen of nematodes. BMC Biology, 17, 10. (doi: 10.1186/s12915-019-0632-x) (PMID:30813925) (PMCID:PMC6394051)

2017

McIntosh, A., Meikle, L. M., Ormsby, M. J., McCormick, B. A., Christie, J. M. , Brewer, J. M. , Roberts, M. and Wall, D. M. (2017) SipA activation of caspase-3 is a decisive mediator of host cell survival at early stages of Salmonella enterica serovar typhimurium Infection. Infection and Immunity, 85(9), e00393-17. (doi: 10.1128/IAI.00393-17) (PMID:28630067) (PMCID:PMC5563584)

2013

Rezuchova, B., Homerova, D., Sevcikova, B., Novakova, R., Feckova, L., Roberts, M. and Kormanec, J. (2013) Phenotypic analysis of salmonella enterica serovar typhimurium rpoE mutants encoding RNA polymerase extracytoplasmic stress response sigma factors σE with altered promoter specificity. Archives of Microbiology, 195(1), pp. 27-36. (doi: 10.1007/s00203-012-0843-9)

2011

Fookes, M. et al. (2011) Salmonella bongori provides insights into the evolution of the Salmonellae. PLoS Pathogens, 7(8), e1002191. (doi: 10.1371/journal.ppat.1002191)

Sherry, A.E., Inglis, N.F., Stevenson, A., Fraser-Pitt, D.G.E., Everest, P. , Smith, D. and Roberts, M. (2011) Characterisation of proteins extracted from the surface of Salmonella Typhimurium grown under SPI-2-inducing conditions by LC-ESI/MS/MS sequencing. Proteomics, 11(3), pp. 361-370. (doi: 10.1002/pmic.200900802) (PMID:21268266)

Homerova, D., Rezuchova, B., Stevenson, A., Skovierova, H., Roberts, M. and Kormanec, J. (2011) Characterization of the micA gene encoding a small regulatory σE-dependent RNA in Salmonella enterica serovar Typhimurium. Folia Microbiologica, 56(1), pp. 59-65. (doi: 10.1007/s12223-011-0014-4) (PMID:21394477)

Humphrey, S., MacVicar, T., Stevenson, A., Roberts, M., Humphrey, T.J. and Jepson, M.A. (2011) SulA-induced filamentation in Salmonella enterica serovar Typhimurium: effects on SPI-1 expression and epithelial infection. Journal of Applied Microbiology, 111(1), pp. 185-196. (doi: 10.1111/j.1365-2672.2011.05022.x)

Noofeli, M., Bokhari, H., Blackburn, P., Roberts, M., Coote, J.G. and Parton, R.G. (2011) BapC autotransporter protein is a virulence determinant of Bordetella pertussis. Microbial Pathogenesis, 51(3), pp. 169-177. (doi: 10.1016/j.micpath.2011.04.004)

Rowley, G., Skovierova, H., Stevenson, A., Rezuchova, B., Homerova, D., Lewis, C., Sherry, A., Kormanec, J. and Roberts, M. (2011) The periplasmic chaperone Skp is required for successful Salmonella Typhimurium infection in a murine typhoid model. Microbiology, 157(3), pp. 848-858. (doi: 10.1099/mic.0.046011-0) (PMID:21148205)

2010

Kenyon, W.J., Humphreys, S., Roberts, M. and Spector, M.P. (2010) Periplasmic peptidyl-prolyl isomerases SurA and FkpA play an important role in the starvation-stress response (SSR) of Salmonella enterica serovar Typhimurium. Antonie van Leeuwenhoek, 98(1), pp. 51-63. (doi: 10.1007/s10482-010-9428-2)

2009

Rezuchova, B., Skovierova, H., Homerova, D., Roberts, M. and Kormanec, J. (2009) A mutant of Salmonella enterica serovar Typhimurium RNA polymerase extracytoplasmic stress response sigma factor σE with altered promoter specificity. Molecular Genetics and Genomics, 282(2), pp. 119-129. (doi: 10.1007/s00438-009-0450-x)

Lewis, C., Skovierova, H., Rowley, G., Rezuchova, B., Homerova, D., Stevenson, A., Spencer, J., Farn, J., Kormanec, J. and Roberts, M. (2009) Salmonella enterica Serovar Typhimurium HtrA: regulation of expression and role of the chaperone and protease activities during infection. Microbiology, 155(3), pp. 873-881. (doi: 10.1099/mic.0.023754-0)

2008

Cooke, F.J., Brown, D.J., Fookes, M., Pickard, D., Ivens, A., Wain, J., Roberts, M., Kingsley, R.A., Thomson, N.R. and Dougan, G. (2008) Characterization of the genomes of a diverse collection of Salmonella enterica serovar Typhimurium definitive phage type 104. Journal of Bacteriology, 190(24), pp. 8155-8162. (doi: 10.1128/JB.00636-08)

Thomson, N.R. et al. (2008) Comparative genome analysis of Salmonella Enteritidis PT4 and Salmonella Gallinarum 287/91 provides insights into evolutionary and host adaptation pathways. Genome Research, 18(10), pp. 1624-1637. (doi: 10.1101/gr.077404.108)

Lewis, C., Skovierova, H., Rowley, G., Rezuchova, B., Homerova, D., Stevenson, A., Sherry, A., Kormanec, J. and Roberts, M. (2008) Small outer-membrane lipoprotein, SmpA, is regulated by sigmaE and has a role in cell envelope integrity and virulence of Salmonella enterica serovar typhimurium. Microbiology, 154(3), pp. 979-88. (doi: 10.1099/mic.0.2007/011999-0)

2007

McMeechan, A., Roberts, M., Cogan, T.A., Jorgensen, F., Stevenson, A., Lewis, C., Rowley, G. and Humphrey, T.J. (2007) Role of the alternative sigma factors sigma(E) and sigma(S) in survival of Salmonella enterica serovar Typhimurium during starvation, refrigeration and osmotic shock. Microbiology: SGM, 153, pp. 263-269. (doi: 10.1099/mic.0.29235-0)

2006

Skovierova, H., Rezuchova, B., Homerova, D., Roberts, M. and Kormanec, J. (2006) Characterization of the sigmaE-dependent rpoEp3 promoter of Salmonella enterica serovar Typhimurium. FEMS Microbiology Letters, 261(1), pp. 53-59. (doi: 10.1111/j.1574-6968.2006.00325.x)

Skovierova, H., Rezuchova, B., Homerova, D., Roberts, M. and Kormanec, J. (2006) Characterization of the σ^E-dependent rpoEp3 promoter of Salmonella enteric serovar Typhimurium. FEMS Microbiology Letters, 261(1), pp. 53-59. (doi: 10.1111/j.1574-6968.2006.00325.x)

Rowley, G., Spector, M., Kormanec, J. and Roberts, M. (2006) Pushing the envelope: extracytoplasmic stress responses in bacterial pathogens. Nature Reviews Microbiology, 4, pp. 383-394. (doi: 10.1038/nrmicro1394)

Skovierova, H., Rowley, G., Rezuchova, B., Homerova, D., Lewis, C., Roberts, M. and Kormanec, J. (2006) Identification of the sigma(E) regulon of Salmonella enterica serovar Typhimurium. Microbiology: SGM, 152, pp. 1347-1359. (doi: 10.1099/mic.0.28744-0)

2005

Rowley, G., Stevenson, A., Kormanec, J. and Roberts, M. (2005) Effect of inactivation of degS on Salmonella enterica serovar Typhimurium in vitro and in vivo. Infection and Immunity, 73, pp. 459-463. (doi: 10.1128/IAI.73.1.459-463.2005)

2004

Miticka, H., Rezuchova, B., Homerova, D., Roberts, M. and Kormanec, J. (2004) Identification of nucleotides critical for activity of the sigma(E)-dependent rpoEp3 promoter in Salmonella enterica serovar Typhimurium. FEMS Microbiology Letters, 238(1), pp. 227-233. (doi: 10.1111/j.1574-6968.2004.tb09760.x)

Farn, J. and Roberts, M. (2004) Effect of inactivation of the HtrA-like serine protease DegQ on the virulence of Salmonella enterica serovar typhimurium in mice. Infection and Immunity, 72, pp. 7357-7359.

Humphreys, S., Rowley, G., Stevenson, A., Anjum, M., Woodward, M., Gilbert, S., Kormanec, J. and Roberts, M. (2004) Role of the two-component regulator CpxAR in the virulence of Salmonella entetica serotype typhimurium. Infection and Immunity, 72, pp. 4654-4661. (doi: 10.1128/IAI.72.8.4654-4661.2004)

Stevenson, A. and Roberts, M. (2004) Intranasal immunisation against tetanus with an attenuated Bordetella bronchiseptica vector expressing FrgcC: improved immunogenicity using a Bvg-regulated promoter to express FrgC. Vaccine, 22, pp. 4300-4305. (doi: 10.1016/j.vaccine.2004.04.020)

2003

Miticka, H., Rowley, G., Rezuchova, B., Homerova, D., Humphreys, S., Farn, J., Roberts, M. and Kormanec, J. (2003) Transcriptional analysis of the rpoE gene encoding extracytoplasmic stress response sigma factor σ^E in Salmonella enteric serovar Typhimurium. FEMS Microbiology Letters, 226(2), pp. 307-314. (doi: 10.1016/S0378-1097(03)00600-1)

Rezuchova, B., Miticka, H., Homerova, D., Roberts, M. and Kormanec, J. (2003) New members of the Escherichia coli sigma(E) regulon identified by a two-plasmid system. FEMS Microbiology Letters, 225(1), pp. 1-7. (doi: 10.1016/S0378-1097(03)00480-4)

Stevenson, A. and Roberts, M. (2003) Use of Bordetella bronchiseptica and Bordetella pertussis as live vaccines and vectors for heterologous antigens. FEMS Immunology and Medical Microbiology, 37, pp. 121-128. (doi: 10.1016/S0928-8244(03)00068-3)

Humphreys, S., Rowley, G., Stevenson, A., Kenyon, W., Spector, M. and Roberts, M. (2003) Role of periplasmic peptidylprolyl isomerases in Salmonella enterica serovar typhimurium virulence. Infection and Immunity, 71, pp. 5386-5388. (doi: 10.1128/IAI.71.9.5386-5388.2003)

Parham, N., Spencer, J., Taylor, D., Ternent, H., Innocent, G., Mellor, D., Roberts, M. and Williams, A. (2003) An adapted ImmunoMagnetic cell Separation method for use in quantification of Escherichia coli O157 : H7 from bovine faeces. Journal of Microbiological Methods, 53, pp. 1-9.

Stevenson, A., MacDonald, J. and Roberts, M. (2003) Cloning and characterisation of type 4 fimbrial genes from Actinobacillus pleuropneumoniae. Veterinary Microbiology, 92, pp. 121-134.

2002

Kenyon, W.J., Sayers, D.G., Humphreys, S., Roberts, M. and Spector, M.P. (2002) The starvation-stress response of Salmonella enterica serovar Typhimurium requires sigma(E_), but not CpxR-regulated extracytoplasmic functions. Microbiology: SGM, 148, pp. 113-122.

Roberts, M. and Parton, R. (2002) Bordetella pertussis. In: Sussman, M. (ed.) Molecular Medical Microbiology. Academic Press, 1565 -1618. ISBN 9780126775303

Stevenson, A. and Roberts, M. (2002) Use of a rationally attenuated Bordetella bronchiseptica as a live mucosal vaccine and vector for heterologous antigens. Vaccine, 20, pp. 2325-2335.

2001

Everest, P., Wain, J., Roberts, M., Rook, G. and Dougan, G. (2001) The molecular mechanisms of severe typhoid fever. Trends in Microbiology, 9, pp. 316-320.

This list was generated on Fri Apr 19 06:00:49 2024 BST.

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