Use of Liquid Nitrogen

The following information and instructions supplement the Safety Policy for the School, and are key elements to ensure that the Safety Policy is implemented effectively.

• Do not accompany flasks of liquid nitrogen in elevators.
• When using or pouring liquefied gas, be careful not to spill on to exposed clothing or shoes. Contact may cause severe burns.
• Wear loose-fitting protective gloves when pouring, and avoid contact with skin. Wearing a transparent face shield is advised. Do not wear sandals or open shoes.
• Do not touch metal objects which have been immersed in liquefied N2.
• Take care with ampoules which have been stored in liquefied N2. Surround an ampoule with cotton wool or cloth to lessen risk of explosion. Ampoules for storage in liquefied N2 should, wherever possible, have a vent hole. Without a vent hole, there is a risk of explosion.

Low Temperature Cabinets

Protective gloves must be worn at all times when handling contents of cabinet.

Solid CO2

Handle with care as it can cause serious burns. Wear protective gloves at all times.

Do not store in sealed containers: if in a thermos flask, leave bung out, otherwise explosion may occur.

First aid for cryogenic burns

1. Flush the area of skin with tepid water.
2. Do not apply direct heat or use hot water.
3. Do not use a forceful flow of water as this could cause tissue damage.
4. Move the casualty to a warm place and seek medical attention / first aid
5. If the burn is severe, call an ambulance.
6. While waiting for medical attention, continue to flush the area with tepid water, remove tight jewellery.

See also Safety & Environmental Protection Services Guidance Note Ref. Ch007/2010