Dr Nikolaj Gadegaard

- Senior Lecturer (Biomedical Engineering)
telephone: 01413305243
email: Nikolaj.Gadegaard@glasgow.ac.uk
Research interests
Nikolaj graduated from the University of Copenhagen with a BSc in chemistry (1995), MSc in physics (1998) and a PhD in biophysics (2002). The research work during the Master’s and the PhD was carried out at Risø National Laboratory.
He moved to Glasgow in 2002 to take up a PDRA position in the Centre for Cell Engineering and held a personal research fellowship from the Royal Society of Edinburgh (2003-2006). He was appointed to his current position as a lecturer in 2006.
He is an academic member of the Centre for Cell Engineering and is heading the fabrication activities within the group. His current research is focused on nanostructured surfaces by electron beam lithography for cell and tissue engineering applications. This involves novel patterns to control stem cell differentiation and next generation of lithographically defined 3D scaffolds for tissue engineering. With Chemistry he provides lithographic platforms for supramolecular self-assembly.
2012
Abdulrahman, N.A., Fan, Z., Tonooka, T., Kelly, S.M. , Gadegaard, N. , Hendry, E., Govorov, A.O., and Kadodwala, M. (2012) Induced chirality through electromagnetic coupling between chiral molecular layers and plasmonic nanostructures. Nano Letters, 12 (2). pp. 977-983. ISSN 1530-6984 (doi:10.1021/nl204055r)
2011
Kirchhof, K., Andar, A., Yin, H. , Gadegaard, N. , Riehle, M.O. , and Groth, T. (2011) Polyelectrolyte multilayers generated in a microfluidic device with pH gradients direct adhesion and movement of cells. Lab on a Chip, 11 (19). pp. 3326-3335. ISSN 1473-0197 (doi:10.1039/C1LC20408D)
Kingham, E.J., Tsimbouri, M., Gadegaard, N. , Dalby, M.J. , and Oreffo, R.O.C. (2011) Nanotopography induced osteogenic differentiation of human stem cells. Bone, 48 (Sup. 2). S108-S109. ISSN 8756-3282 (doi:10.1016/j.bone.2011.03.177)
McMurray, R.J., Gadegaard, N. , Tsimbouri, P.M. , Burgess, K.V. , McNamara, L.E. , Tare, R., Murawski, K., Kingham, E., Oreffo, R.O.C., and Dalby, M.J. (2011) Nanoscale surfaces for the long-term maintenance of mesenchymal stem cell phenotype and multipotency. Nature Materials, 10 (8). pp. 637-644. ISSN 1476-1122 (doi:10.1038/nmat3058)
Pedersen, R.H., Scurr, D.J., Roach, P., Alexander, M.R., and Gadegaard, N. (2011) Full-thickness characterization of plasma polymerized hexane films irradiated by an electron beam. Plasma Processes and Polymers, 9 (1). pp. 22-27. ISSN 1612-8850 (doi:10.1002/ppap.201100067)
2010
Altomare, L., Gadegaard, N. , Visai, L., Tanzi, M.C., and Fare, S. (2010) Biodegradable microgrooved polymeric surfaces obtained by photolithography for skeletal muscle cell orientation and myotube development. Acta Biomaterialia, 6 (6). pp. 1948-1957. ISSN 1742-7061 (doi:10.1016/j.actbio.2009.12.040)
Altomare, L., Riehle, M.O. , Gadegaard, N. , Tanzi, M.C., and Farè, S. (2010) Microcontact printing of fibronectin on a biodegradable polymeric surface for skeletal muscle cell orientation. International Journal of Artificial Organs, 33 (8). pp. 535-543. ISSN 0391-3988
Csaderova, L., Martines, E., Seunarine, K. , Gadegaard, N. , Wilkinson, C.D.W. , and Riehle, M.O. (2010) A biodegradable and biocompatible regular nanopattern for large-scale selective cell growth. Small, 6 (23). pp. 2755-2761. ISSN 1613-6810 (doi:10.1002/smll.201000193)
Gaston, A., Khokhar, A.Z. , Bilbao, L., Saez-Martinez, V., Corres, A., Obieta, I., and Gadegaard, N. (2010) Nanopatterned UV curable hydrogels for biomedical applications. Microelectronic Engineering, 87 (5-8). pp. 1057-1061. ISSN 0167-9317 (doi:10.1016/j.mee.2009.11.089)
Hendry, E., Carpy, T., Johnston, J., Popland, M., Mikhaylovskiy, R.V., Lapthorn, A.J. , Kelly, S.M. , Barron, L.D. , Gadegaard, N. , and Kadodwala, M. (2010) Ultrasensitive detection and characterization of biomolecules using superchiral fields. Nature Nanotechnology, 5 (11). pp. 783-787. ISSN 1748-3387 (doi:10.1038/nnano.2010.209)
Ji, B., Cusack, M. , Freer, A., Dobson, P.S. , Gadegaard, N. , and Yin, H. (2010) Control of crystal polymorph in microfluidics using molluscan 28 kDa Ca2+-binding protein. Integrative Biology, 2 . pp. 528-535. ISSN 1757-9694 (doi:10.1039/c0ib00007h)
Majani, R., Zelzer, M., Gadegaard, N. , Rose, F.R., and Alexander, M.R. (2010) Preparation of Caco-2 cell sheets using plasma polymerised acrylic acid as a weak boundary layer. Biomaterials, 31 (26). pp. 6764-6771. ISSN 0142-9612 (doi:10.1016/j.biomaterials.2010.05.049)
McMurray, R.J., Gadegaard, N. , Dalby, M.J. , Tsimbouri, M.P., Maclaine, S. , Meek, D., McNamara, L.E. , Child, H., and Berry, C.C. (2010) Research highlights. Nanomedicine, 5 (10). pp. 1495-1499. ISSN 1743-5889 (doi:10.2217/nnm.10.119)
Pedersen, R.H., Hamzah, M., Thoms, S. , Roach, P., Alexander, M.R., and Gadegaard, N. (2010) Electron beam lithography using plasma polymerized hexane as resist. Microelectronic Engineering, 87 (5-8). pp. 1112-1114. ISSN 0167-9317 (doi:10.1016/j.mee.2009.11.043)
Roach, P., Parker, T., Gadegaard, N. , and Alexander, M.R. (2010) Surface strategies for control of neuronal cell adhesion: A review. Surface Science Reports, 65 (6). pp. 145-173. ISSN 0167-5729 (doi:10.1016/j.surfrep.2010.07.001)
Sun, T., Donoghue, P.S. , Higginson, J.R. , Gadegaard, N. , Barnett, S.C. , and Riehle, M.O. (2010) The interactions of astrocytes and fibroblasts with defined pore structures in static and perfusion cultures. Biomaterials, 32 (8). pp. 2021-2031. ISSN 0142-9612 (doi:10.1016/j.biomaterials.2010.11.046)
2009
Kantawong, F., Burchmore, R.J.S. , Gadegaard, N. , Oreffo, R.O.C., and Dalby, M.J. (2009) Proteomic analysis of human osteoprogenitor response to disordered nanotopography. Journal of the Royal Society: Interface . ISSN 1742-5689 (doi:10.1098/rsif.2008.0447)
Biggs, M.J.P., Richards, R.G., Gadegaard, N. , McMurray, R.J., Affrosman, S., Wilkinson, C.D. , Oreffo, R.O.C., and Dalby, M.J. (2009) Interactions with nanoscale topography: adhesion quantification and signal transduction in cells of osteogenic and multipotent lineage. Journal of Biomedical Materials Research Part A, 91A (1). pp. 195-208. ISSN 1549-3296 (doi:10.1002/jbm.a.32196)
Biggs, M.J.P., Richards, R.G., Gadegaard, N. , Wilkinson, C.D.W. , Oreffo, R.O.C., and Dalby, M.J. (2009) The use of nanoscale topography to modulate the dynamics of adhesion formation in primary osteoblasts and ERK/MAPK signalling in STRO-1+ enriched skeletal stem cells. Biomaterials, 30 (28). 5094-5103 . ISSN 0142-9612 (doi:10.1016/j.biomaterials.2009.05.049)
Malm, J., Giannaras, D., Riehle, M.O. , Gadegaard, N. , and Sjovall, P. (2009) Fixation and drying protocols for the preparation of cell samples for time-of-flight secondary ion mass spectrometry analysis. Analytical Chemistry, 81 (17). pp. 7107-7205. ISSN 0003-2700 (doi:10.1021/ac900636v)
Kantawong, F., Burgess, K.E.V. , Jayawardena, K., Hart, A. , Burchmore, R.J. , Gadegaard, N. , Oreffo, R.O.C., and Dalby, M.J. (2009) Whole proteome analysis of osteoprogenitor differentiation induced by disordered nanotopography and mediated by ERK signalling. Biomaterials, 30 (27). pp. 4723-4731. ISSN 0142-9612 (doi:10.1016/j.biomaterials.2009.05.040)
Seunarine, K., Curtis, A.S.G. , Meredith, D.O., Wilkinson, C.D.W. , Riehle, M.O. , and Gadegaard, N. (2009) A hierarchical response of cells to perpendicular micro- and nanometric textural cues. IEEE Transactions on NanoBioscience, 8 (3). pp. 219-225. ISSN 1536-1241 (doi:10.1109/TNB.2009.2016477)
Streb, C., Tsunashima, R., MacLaren, D.A. , McGlone, T., Akutagawa, T., Nakamura, T., Scandurra, A., Pignataro, B., Gadegaard, N. , and Cronin, L. (2009) Supramolecular silver polyoxometalate architectures direct the growth of composite semiconducting nanostructures. Angewandte Chemie (International Edition), 48 (35). pp. 6490-6493. ISSN 1433-7851 (doi:10.1002/anie.200901650)
Rossi, L., Riehle, M.O. , McNamara, L.E., Burchmore, R.J. , Dalby, M.J. , McMurray, R.J., Gadegaard, N. , Ahmed, S.R., and Tsimbouri, P. (2009) Research highlights. Nanomedicine, 4 (6). pp. 613-617. ISSN 1743-5889 (doi:10.2217/nnm.09.46)
Glidle, A., Hillman, A.R., Rydeo, K.S., Smith, E.L., Cooper, J.M. , Gadegaard, N. , Webster, J.R.P., Dalgliesh, R., and Cubitt, R. (2009) Use of neutron reflectivity to measure the dynamics of solvation and structural changes in polyvinylferrocene films during electrochemically controlled redox cycling. Langmuir, 25 (7). pp. 4093-4103. ISSN 0743-7463 (doi:10.1021/la803234e)
Krasa, D., Wilkinson, C.D.W. , Gadegaard, N. , Kong, X., Zhou, H. , Roberts, A.P., Muxworthy, A.R., and Williams, W. (2009) Nanofabrication of two-dimensional arrays of magnetite particles for fundamental rock magnetic studies. Journal of Geophysical Research. Solid Earth, 114 (B02104). ISSN 0148-0227 (doi:10.1029/2008JB006017)
Lamond, R., Donoghue, P. , Gadegaard, N. , Riehle, M. , and Barnett, S. (2009) Developing a biodegradable construct to enhance axonal alignment following a spinal cord injury. Glia, 57 (13). S162-S162. ISSN 0894-1491
MacLaren, D.A., Johnston, J., Duncan, D.A., Marchetto, H., Dhesi, S.S., Gadegaard, N. , and Kadodwala, M. (2009) Asymmetric photoelectron transmission through chirally-sculpted, polycrystalline gold. Physical Chemistry Chemical Physics, 11 (38). pp. 8413-8416. ISSN 1463-9076 (doi:10.1039/b911447e)
Ross, L., Riehle, M.O. , McNamara, L.E. , Burchmore, R.J.S. , Dalby, M.J. , McMurray, R.J., Gadegaard, N. , Ahmed, S., and Tsimbouri, P.M. (2009) Research highlights. Nanomedicine, 4 (6). pp. 613-617. ISSN 1743-5889 (doi:10.2217/nnm.09.46)
Song, Y.F., McMillan, N., Long, D.L. , Kane, S., Malm, J., Riehle, M. , Pradeep, C.P., Gadegaard, N. , and Cronin, L. (2009) Micropatterned Surfaces with Covalently Grafted Unsymmetrical Polyoxometalate-Hybrid Clusters Lead to Selective Cell Adhesion. Journal of the American Chemical Society, 131 (4). pp. 1340-1341. ISSN 0002-7863 (doi:10.1021/ja807091v)
Yang, J., Rose, F.R.A.J., Gadegaard, N. , and Alexander, M. (2009) A high-throughput assay of cell-surface interactions using topographical and chemical gradients. Advanced Materials, 21 (3). pp. 300-304. ISSN 0935-9648 (doi:10.1002/adma.200801942)
Yang, J., Rose, F.R.A.J., Gadegaard, N. , and Alexander, M.R. (2009) Effect of sessile drop volume on the wetting anisotropy observed on grooved surfaces. Langmuir, 25 (5). pp. 2567-2571. ISSN 0743-7463 (doi:10.1021/la803942h)
Yin, H., Bozhi, J., Dobson, P.S. , Mosbahi, K. , Glidle, A. , Gadegaard, N. , Freer, A., Cooper, J.M. , and Cusack, M. (2009) Screening of biomineralization using microfluidics. Analytical Chemistry, 81 (1). pp. 473-478. ISSN 0003-2700 (doi:10.1021/ac801980b)
Zelzer, M., Scurr, D., Abdullah, B., Urquhart, A.J., Gadegaard, N. , Bradley, J.W., and Alexander, M.R. (2009) Influence of the plasma sheath on plasma polymer deposition in advance of a mask and down pores. Journal of Physical Chemistry B, 113 (25). pp. 8487-8494. ISSN 1520-6106 (doi:10.1021/jp902137y)
2008
Dalby, M.J., McNamara, L.E. , Riehle, M.O. , Burchmore, R.J.S. , Kuntanawat, P., McMurray, R.J., Gadegaard, N. , and Andar, A. (2008) Research highlights. Nanomedicine, 3 (4). pp. 411-414. ISSN 1743-5889 (doi:10.2217/17435889.3.4.411)
Fleming, C., Long, D. L. , McMillan, N., Johnston, J., Bovet, N., Dhanak, V., Gadegaard, N. , Kogerler, P., Cronin, L. , and Kadodwala, M. (2008) Reversible electron-transfer reactions within a nanoscale metal oxide cage mediated by metallic substrates. Nature Nanotechnology, 3 (4). pp. 229-233. ISSN 1748-3387 (doi:10.1038/nnano.2008.66)
Dalby, M.J., Gadegaard, N. , and Wilkinson, C.D.W. (2008) The response of fibroblasts to hexagonal nanotopography fabricated by electron beam lithography. Journal of Biomedical Materials Research Part A, 84A (4). pp. 973-979. ISSN 1549-3296 (doi:10.1002/jbm.a.31409)
Dalby, M.J., Gadegaard, N. , and Wilkinson, C.D.W. (2008) The response of fibroblasts to hexagonal nanotopography fabricated by electron beam lithography. Journal of Biomedical Materials Research Part A, 84A (4). pp. 973-979. ISSN 1549-3296 (doi:10.1002/jbm.a.31409)
Gadegaard, N., Chen, X.Y., Rutten, F.J.M., and Alexander, M.R. (2008) High-energy electron beam lithography of octadecylphosphonic acid monolayers on aluminum. Langmuir, 24 (5). pp. 2057-2063. ISSN 0743-7463 (doi:10.1021/la701733n)
Gadegaard, N., Dalby, M.J. , Riehle, M.O. , and Wilkinson, C.D.W. (2008) Optimizing substrate disorder for bone tissue engineering of mesenchymal stem cells. Journal of Vacuum Science and Technology B, 26 (6). pp. 2554-2557. ISSN 1071-1023 (doi:10.1116/1.2978407)
Gadegaard, N., Seunarine, K. , Smith, D.J.A., Meredith, D., Wilkinson, C.D.W. , and Riehle, M.O. (2008) A hybrid three-dimensional nanofabrication method for producing vascular tissue engineering scaffold. Japanese Journal of Applied Physics, 47 (9). pp. 7415-7419. ISSN 0021-4922 (doi:10.1143/JAPP.47.7415)
Seunarine, K., Meredith, D.O., Riehle, M.O. , Wilkinson, C.D.W. , and Gadegaard, N. (2008) Biodegradable polymer tubes with litho graphically controlled 3D micro- and nanotopography. Microelectronic Engineering, 85 (5-6). pp. 1350-1354. ISSN 0167-9317 (doi:10.1016/j.mee.2008.02.002)
Song, Y.F., McMillan, N., Long, D.L. , Thiel, J., Ding, Y.L., Chen, H.S., Gadegaard, N. , and Cronin, L. (2008) Design of hydrophobic polyoxometalate hybrid assemblies beyond surfactant encapsulation. Chemistry: a European Journal, 14 (8). pp. 2349-2354. ISSN 0947-6539 (doi:10.1002/chem.200701629)
2007
Biggs, M.J.P., Richards, R.G., Gadegaard, N. , Wilkinson, C.D.W. , and Dalby, M.J. (2007) Regulation of implant surface cell adhesion: Characterization and quantification of S-phase primary osteoblast adhesions on biomimetic nanoscale substrates. Journal of Orthopaedic Research, 25 . pp. 273-282. (doi:10.1002/jor.20319)
Biggs, M.J.P., Richards, R.G., Gadegaard, N. , Wilkinson, C.D.W. , and Dalby, M.J. (2007) The effects of nanoscale pits on primary human osteoblast adhesion formation and cellular spreading. Journal of Materials Science: Materials in Medicine, 18 . pp. 399-404. ISSN 1573-4838 (doi:10.1007/s10856-006-0705-6)
Bovet, N., McMillan, N., Gadegaard, N. , and Kadodwala, M. (2007) Supramolecular assembly facilitating adsorbate-induced chiral electronic states in a metal surface. Journal of Physical Chemistry B, 111 . pp. 10005-10011. (doi:10.1021/jp074056s)
Dalby, M.J., Gadegaard, N. , Tare, R., Andar, A., Riehle, M.O. , Herzyk, P. , Wilkinson, C.D.W. , and Oreffo, R.O.C. (2007) The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder. Nature Materials, 6 (12). pp. 997-1003. ISSN 1476-1122 (doi:10.1038/nmat2013)
Dalby, M.J., Biggs, M.J.P., Gadegaard, N. , Kalna, G., Wilkinson, C.D.W. , and Curtis, A.S.G. (2007) Nanotopographical stimulation of mechanotransduction and changes in interphase centromere positioning. Journal of Cellular Biochemistry, 100 . pp. 326-338. (doi:10.1002/jcb.21058)
Dalby, M.J., Gadegaard, N. , Herzyk, P. , Agheli, H., Sutherland, D.S., and Wilkinson, C.D.W. (2007) Group analysis of regulation of fibroblast genome on low-adhesion nanostructures. Biomaterials, 28 . pp. 1761-1769. (doi:10.1016/j.biomaterials.2006.11.049)
2006
Curtis, A.S.G., Dalby, M.J. , and Gadegaard, N. (2006) Cell signaling arising from nanotopography: implications for nanomedical devices. Nanomedicine, 1 . pp. 67-72. (doi:10.2217/17435889.1.1.67)
Gadegaard, N (2006) Atomic force microscopy in biology: technology and techniques. Biotechnic and Histochemistry, 81 . pp. 87-97. (doi:10.1080/10520290600783143)
Pranov, H, Rasmussen, HK, Larsen, NB, and Gadegaard, N (2006) On the injection molding of nanostructured polymer surfaces. Polymer Engineering and Science, 46 . pp. 160-171. (doi:10.1002/pen.20459)
Seunarine, K, Gadegaard, N , Tormen, M, O Meredith, D, O Riehle, M, and Wilkinson, CDW (2006) 3D polymer scaffolds for tissue engineering. Nanomedicine, 1 . pp. 281-296. (doi:10.2217/17435889.1.3.281)
2005
Glidle, A., Hadyoon, C.S., Gadegaard, N. , Cooper, J.M. , Hillman, A.R., Wilson, A.R., Ryder, K.S., Webster, J.R.P., and Cubitt, R. (2005) Evaluating the influence of deposition conditions on solvation of reactive conducting polymers with neutron reflectivity. Journal of Physical Chemistry B, 109 (30). pp. 14335-14343. ISSN 1520-6106 (doi:10.1021/jp0515030)
2004
Gadegaard, N., Dalby, M.J. , Riehle, M.O. , Curtis, A.S.G. , and Affrossman, S. (2004) Tubes with controllable internal nanotopography. Advanced Materials, 20 . pp. 1857-1860. ISSN 0935-9648 (doi:10.1002/adma.200400408)
Dalby, M.J., Gadegaard, N. , Riehle, M.O. , Wilkinson, C.D.W., and Curtis, A.S.G. (2004) Investigating filopodia sensing using arrays of defined nano-pits down to 35 nm diameter in size. International Journal of Biochemistry and Cell Biology, 36 (10). pp. 2005-2015. ISSN 1357-2725 (doi:10.1016/j.biocel.2004.03.001)
Curtis, A.S.G., Gadegaard, N. , Dalby, M.J. , Riehle, M.O. , Wilkinson, C.D.W., and Aitchison, G. (2004) Cells react to nanoscale order and symmetry in their surroundings. IEEE Transactions on NanoBioscience, 3 (1). pp. 61-65. ISSN 1536-1241 (doi:10.1109/TNB.2004.824276)
Dalby, M.J., Giannaras, D., Riehle, M.O. , Gadegaard, N. , Affrossman, S., and Curtis, A.S.G. (2004) Rapid fibroblast adhesion to 27 nm high polymer demixed nano-topography. Biomaterials, 25 (1). pp. 77-83. ISSN 0142-9612 (doi:10.1016/S0142-9612(03)00475-7)
Cooper, J.M., Cubitt, R., Dalgliesh, R.M., Gadegaard, N. , Glidle, A. , Hillman, A.R., Mortimer, R.J., Ryder, K.S., and Smith, E.L. (2004) Dynamic in situ electrochemical neutron reflectivity measurements. Journal of the American Chemical Society, 126 (47). pp. 15362-15363. ISSN 0002-7863 (doi:10.1021/ja044682s)
2003
Gadegaard, N, Thoms, S , Macintyre, DS , Mcghee, K, Gallagher, J, Casey, B, and Wilkinson, CDW (2003) Arrays of nano-dots for cellular engineering. Microelectronic Engineering, 67-8 . pp. 162-168. (doi:10.1016/S0167-9317(03)00067-4)
2002
Hillman, A.R., Bailey, L., Glidle, A. , Cooper, J.M. , Gadegaard, N. , and Webster, J.R.P. (2002) Spatial distributions of polymer and mobile species in poly(o-toluidine) films. Journal of Electroanalytical Chemistry, 532 . pp. 269-276. ISSN 1572-6657
