Professor Richard Hartley

  • Professor of Chemical Biology (Chemistry)

telephone: 01413304398
email: Richard.Hartley@glasgow.ac.uk

Research interests

Research Interests

The primary area of research in the Hartley group is chemical biology, i.e., the use of synthetic organic chemistry to unlock the secrets of biology.  We do this in two key ways: (a) we design, make and use small molecular probes to study oxidative stress and ageing (b) we find new ways to make large numbers of  drug-like compounds, through titanium carbenoid chemistry and by a platform technology that we call MPEG-assised organic synthesis. Our research is both multidisciplinary and relevant to industry, and has given rise to an SME, Antoxis.  All my PhD students have submitted within four years of starting work in my laboratory, all have been awarded their PhDs

For an interview with Dr Hartley on Healthy Ageing and Tackling Oxidative Stress (Interview in ENGLISH, introduction in SPANISH) click here.

Research Topics

  • Reactive oxygen species (ROS) and calcium ions in homeostasis and aging (ageing)
  • Intracellular targeting in particular to mitochondria
  • Molecular probes and functional molecules including photoactivatable and caged molecules
  • Technologies to facilitate clean synthesis and array synthesis
  • Titanium carbenoids

Publication Highlights

  • A prototypical small molecule modulator uncouples mitochondria in response to endogenous hydrogen peroxide production. McQuaker, S. J.; Quinlan, C. L.; Caldwell, S. T.; Brand, M. D.; Hartley, R. C. ChemBioChem 2013, 14,  901-1013 (front cover and doi:10.1002/cbic.201300115).
  • Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore.  Chalmers, S.; Caldwell, S. T.; Quin, C.; Prime, T. A.; James, A. M.; Cairns, A. G.; Murphy, M. P.; McCarron, J. G.; Hartley, R. C. J. Am. Chem. Soc.  2012, 134, 758-761(doi: 10.1021/ja2077922). 
  • Using the mitochondria-targeted ratiometric mass spectrometry probe MitoB to measure H2O2 in living Drosophila. Cochemé, H. M.; Logan, A.; Prime, T. A.;  Abakumova, I.; Quin, C.; McQuaker, S. J.; Patel, J. V.; Fearnley, I. M.; James, A. M.; Porteous, C. M.; Smith, R. A. J.; Hartley, R. C.; Partridge, L.; Murphy, M. P.  Nature Protocols 2012, 7, 946-958 (doi:10.1038/nprot.2012.035)
  • Measurement of H2O2 within living Drosophila during aging using a ratiometric mass spectrometry probe targeted to the mitochondrial matrix Cochemé, H. M.; Quin, C.; McQuaker, S. J.; Cabreiro, F.; Logan, A.; Prime, T. A.;  Abakumova, I.; Patel, J. V.; Fearnley, I. M.; James, A. M.; Porteous, C. M.; Smith, R. A. J.; Saeed, S.; Carré, J. E.; Singer, M.; Gems, D.; Hartley, R. C.; Partridge, L.; Murphy, M. P. Cell Metabolism 2011, 13, 340-350 (doi: 10.1016/j.cmet.2011.02.003).
  • Low Molecular WeightMPEG-assisted Organic Synthesis Figlus, M.; Tarruella, A. C.; Messer, A.; Sollis, S. L.; Hartley, R. C. Chemical Communications 2010, 46, 4405-4407 (doi:10.1039/C0CC00415D).

 

Richard Hartley research group photo May 2013 


Teaching

Teaching

  • Level-3 Mechanistic Organic Chemistry
  • Level-4 Chemical Biology
  • Level-4 Medicial chemistry of cancer

Additional information

Awards

MA (Cantab), PhD, FRSC

Selected Recent Talks

  • "Biología Quimíca: Senalizadores y Sensores Quimicos." Congresso Internacional Biología, Química y Agronomía. Guadalajara, Mexico, September 2013.
  • "Targeting Mitochondria by ‘Magic Molecules’: Strategic Role of Chemistry."  3rd World Congress on Targeting Mitochondria, Berlin (Germany), November 2012.
  • "Mitochondria-targeted Probes and Functional Molecules to Test the Free Radical Theory of Ageing and Understand Smooth Muscle Contraction" ISACS5, Manchester (UK), July 2011
  • "Mitochondria-targeted Probes and Functional Molecules" EB2011 in Washington DC, April 2011
  • Lectures at five other international conferences 2008-13 [COST action CM0902 (2012), 42nd IUPAC conference (2009), GRC Combinatorial Chemistry, High Throughput Chemistry & Chemical Biology (2009), SPIN 2008 (2008), 37th Annual Meeting of the American Aging Association (2008)].

External Appointments & Positions

  • Spin out company (Antoxis, see http://www.antoxis.com/): provided its starting research basis.
  • Invited discussion leader and session chair of "Drug Discovery and Enabling Technology" atHigh Throughput Chemistry & Chemical Biology, Gordon Research Conference, New London (NH), June 2011, and invited expert panel member at 3rd World Congress on Targeting Mitochondria, Berlin (Germany), November 2012.
  • Session chair at three other conferences [ISACS5 (2011), OMCOS 15 (2009); GRC Combinatorial Chemistry (2009)]
  • Organiser of conferences on the chemical biology of ageing and oxidative stress: ChemAgeIII (RSC, London, April 2006), Radical Chemistry and Biology - oxidative stress and ageing (Glasgow, March 2007).
  • Representative in British delegation to American Ageing association meeting in Colorado 2008
  • EPSRC panel member.

Other Notable Achievements

  • Originator of ‘Protection Hypothesis’ in behavioral ecology
  • First and currently best probe for quantify hydrogen peroxide in the mitochondria of living organisms (MitoB)

 


All publications

List by: Type | Date

Jump to: 2017 | 2016 | 2015 | 2014 | 2013 | 2012 | 2011 | 2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002 | 2001
Number of items: 57.

2017

Arndt, S. et al. (2017) Assessment of H2S in vivo using the newly developed mitochondria-targeted mass spectrometry probe MitoA. Journal of Biological Chemistry, (doi:10.1074/jbc.M117.784678) (Early Online Publication)

Salin, K., Auer, S. K., Villasevil, E. M., Anderson, G. J., Cairns, A. G., Mullen, W. , Hartley, R. C. and Metcalfe, N. B. (2017) Using the MitoB method to assess levels of reactive oxygen species in ecological studies of oxidative stress. Scientific Reports, 7, 41228. (doi:10.1038/srep41228)

2016

Buskiewicz, I. A. et al. (2016) Reactive oxygen species induce virus-independent MAVS-oligomerization in systemic lupus erythematosus. Science Signaling, 9(456), ra115. (doi:10.1126/scisignal.aaf1933) (PMID:27899525)

McGarry, D. J., Shchepinova, M. M., Lillla, S., Hartley, R. C. and Olson, M. F. (2016) A cell-permeable biscyclooctyne as a novel probe for the identification of protein sulfenic acids. ACS Chemical Biology, 11(12), pp. 3300-3304. (doi:10.1021/acschembio.6b00742) (PMID:27792307)

2015

Caldwell, S. T., Cairns, A. G., Olson, M., Chalmers, S., Sandison, M., Mullen, W., McCarron, J. G. and Hartley, R. C. (2015) Synthesis of an azido-tagged low affinity ratiometric calcium sensor. Tetrahedron, 71(51), pp. 9571-9578. (doi:10.1016/j.tet.2015.10.052) (PMID:26709317) (PMCID:PMC4660056)

Robb, E. L. et al. (2015) Selective superoxide generation within mitochondria by the targeted redox cycler MitoParaquat. Free Radical Biology and Medicine, 89, pp. 883-894. (doi:10.1016/j.freeradbiomed.2015.08.021) (PMID:26454075)

Salin, K., Auer, S. k., Rudolf, A. M., Anderson, G. J., Cairns, A. G., Mullen, W., Hartley, R. C. , Selman, C. and Metcalfe, N. B. (2015) Individuals with higher metabolic rates have lower levels of reactive oxygen species in vivo. Biology Letters, 11(9), 20150538. (doi:10.1098/rsbl.2015.0538) (PMID:26382073)

Cairns, A. G., McQuaker, S. J., Murphy, M. P. and Hartley, R. C. (2015) Targeting mitochondria with small molecules: the preparation of MitoB and MitoP as exomarkers of mitochondrial hydrogen peroxide. In: Weissig, V. and Edeas, M. (eds.) Mitochondrial Medicine: Volume II, Manipulating Mitochondrial Function. Series: Methods in Molecular Biology, 1265. Springer New York, pp. 25-50. ISBN 9781493922871 (doi:10.1007/978-1-4939-2288-8_3)

2014

Chouchani, E. T. et al. (2014) Ischaemic accumulation of succinate controls reperfusion injury through mitochondrial ROS. Nature, 515(7527), pp. 431-435. (doi:10.1038/nature13909) (PMID:25383517) (PMCID:PMC4255242)

Logan, A., Shabalina, I. G., Prime, T. A., Rogatti, S., Kalinovich, A. V., Hartley, R. C., Budd, R. C., Cannon, B. and Murphy, M. P. (2014) In vivo levels of mitochondrial hydrogen peroxide increase with age in mtDNA mutator mice. Aging Cell, 13(4), pp. 765-768. (doi:10.1111/acel.12212) (PMID:24621297) (PMCID:PMC4326952)

Cairns, A. G., Senn, H. M., Murphy, M. P. and Hartley, R. C. (2014) Expanding the palette of phenanthridinium cations. Chemistry: A European Journal, 20(13), pp. 3742-3751. (doi:10.1002/chem.201304241) (PMID:24677631) (PMCID:PMC4164275)

Li Pun, P. B. et al. (2014) A mitochondria-targeted mass spectrometry probe to detect glyoxals: implications for diabetes. Free Radical Biology and Medicine, 67, pp. 437-450. (doi:10.1016/j.freeradbiomed.2013.11.025) (PMID:24316194) (PMCID:PMC3978666)

Logan, A. et al. (2014) Using exomarkers to assess mitochondrial reactive species in vivo. Biochimica et Biophysica Acta: General Subjects, 1840(2), pp. 923-930. (doi:10.1016/j.bbagen.2013.05.026) (PMID:23726990)

2013

McQuaker, S. J., Quinlan, C. L., Caldwell, S. T., Brand, M. D. and Hartley, R. C. (2013) A prototypical small-molecule modulator uncouples mitochondria in response to endogenous hydrogen peroxide production. ChemBioChem, 14(8), pp. 993-1000. (doi:10.1002/cbic.201300115) (PMID:23640856) (PMCID:PMC3743171)

Chouchani, E. T. et al. (2013) Cardioprotection by S-nitrosation of a cysteine switch on mitochondrial complex I. Nature Medicine, 19(6), pp. 753-759. (doi:10.1038/nm.3212) (PMID:23708290) (PMCID:PMC4019998)

Sikora, A., Zielonka, J., Adamus, J., Debski, D., Dybala-Defratyka, A., Michalowski, B., Joseph, J., Hartley, R.C., Murphy, M.P. and Kalyanaraman, B. (2013) Reaction between peroxynitrite and triphenylphosphonium-substituted arylboronic acid isomers: identification of diagnostic marker products and biological implications. Chemical Research in Toxicology, 26(6), pp. 856-867. (doi:10.1021/tx300499c) (PMID:23611338) (PMCID:PMC3700382)

2012

Harvey, A. L., Young, L. C., Kornisiuk, E., Snitcofsky, M., Colettis, N., Blanco, C., Jerusalinsky, D., Jamieson, A. G. , Hartley, R. C. and Stone, T. W. (2012) A novel dihydro-pyrazolo(3,4d)(1,2,4)triazolo(1,5a)pyrimidin-4-one (AJ23) is an antagonist at adenosine A1 receptors and enhances consolidation of step-down avoidance. Behavioural Brain Research, 234(2), pp. 184-191. (doi:10.1016/j.bbr.2012.06.023)

Smith, R.A.J., Hartley, R.C., Cochemé, H.M. and Murphy, M.P. (2012) Mitochondrial pharmacology. Trends in Pharmacological Sciences, 33(6), pp. 341-352. (doi:10.1016/j.tips.2012.03.010) (PMID:22521106)

Cochemé, H.M. et al. (2012) Using the mitochondria-targeted ratiometric mass spectrometry probe MitoB to measure H2O2 in living Drosophila. Nature Protocols, 7(5), pp. 946-958. (doi:10.1038/nprot.2012.035) (PMID:22517261)

Caldwell, S.T., McPhail, D.B., Duthie, G.D. and Hartley, R.C. (2012) Synthesis of polyhydroxylated flavonoids bearing a lipophilic decyl tail as potential therapeutic antioxidants. Canadian Journal of Chemistry = Journal canadien de chimie, 90(1), pp. 23-33. (doi:10.1139/V11-087)

Chalmers, S., Caldwell, S. T., Quin, C., Prime, T. A., James, A. M., Cairns, A. G., Murphy, M. P., McCarron, J. G. and Hartley, R. C. (2012) Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore. Journal of the American Chemical Society, 134(2), pp. 758-761. (doi:10.1021/ja2077922) (PMID:22239373) (PMCID:PMC3260739)

Omar, M.H., Mullen, W., Stalmach, A., Auger, C., Rouanet, J.-M., Teissedre, P.-L., Caldwell, S.T., Hartley, R.C. and Crozier, A. (2012) Absorption, disposition, metabolism, and excretion of [3-14C]caffeic acid in rats. Journal of Agricultural and Food Chemistry, 60(20), pp. 5205-5214. (doi:10.1021/jf3001185)

2011

Smith, R. A.J., Hartley, R. C. and Murphy, M. P. (2011) Mitochondria-targeted small molecule therapeutics and probes. Antioxidants and Redox Signaling, 15(12), pp. 3021-3038. (doi:10.1089/ars.2011.3969) (PMID:21395490)

Figlus, M., Wellaway, N., Cooper, A.W.J., Sollis, S.L. and Hartley, R.C. (2011) Synthesis of arrays using low molecular weight MPEG-assisted Mitsunobu reaction. ACS Combinatorial Science, 13(3), pp. 280-285. (doi:10.1021/co100091n)

Cochemé, H.M. et al. (2011) Measurement of H2O2 within living drosophila during aging using a ratiometric mass spectrometry probe targeted to the mitochondrial matrix. Cell Metabolism, 13(3), pp. 340-350. (doi:10.1016/j.cmet.2011.02.003) (PMID:21356523) (PMCID:PMC4413513)

Haahr, A., Rankovic, Z. and Hartley, R. (2011) A one-pot procedure for methylenating carbonyl compounds using the Nysted reagent and titanocene dichloride. Tetrahedron Letters, 52(23), pp. 3020-3022. (doi:10.1016/j.tetlet.2011.04.017)

2010

Quin, C., Robertson, L., McQuaker, S.J., Price, N.C., Brand, M.D. and Hartley, R.C. (2010) Caged mitochondrial uncouplers that are released in response to hydrogen peroxide. Tetrahedron, 66(13), pp. 2384-2389. (doi:10.1016/j.tet.2010.01.103) (PMID:20418941) (PMCID:PMC2852674)

Figlus, M., Tarruella, A.C., Messer, A., Sollis, S.L. and Hartley, R.C. (2010) Low molecular weight MPEG-assisted organic synthesis. Chemical Communications, 46(24), pp. 4405-4407. (doi:10.1039/c0cc00415d)

2009

Quin, C., Trnka, J., Hay, A., Murphy, M.P. and Hartley, R.C. (2009) Synthesis of a mitochondria-targeted spin trap using a novel Parham-type cyclization. Tetrahedron, 65(39), pp. 8154-8160. (doi:10.1016/j.tet.2009.07.081) (PMID:19888470) (PMCID:PMC2767131)

Robertson, L. and Hartley, R. C. (2009) Synthesis of N-arylpyridinium salts bearing a nitrone spin trap as potential mitochondria-targeted antioxidants. Tetrahedron, 65(27), pp. 5284-5292. (doi:10.1016/j.tet.2009.04.083) (PMID:19693262) (PMCID:PMC2722452)

El Fangour, S., Marini, M., Good, J., McQuaker, S.J., Shiels, P.G. and Hartley, R.C. (2009) Nitrones for understanding and ameliorating the oxidative stress associated with aging. Age, 31(4), pp. 269-276. (doi:10.1007/s11357-009-9098-z) (PMID:19479343) (PMCID:PMC2813050)

2008

Main, C.A., Rahman, S.S. and Hartley, R.C. (2008) Synthesis of spiroacetals using functionalised titanium carbenoids. Tetrahedron Letters, 49(32), pp. 4771-4774. (doi:10.1016/j.tetlet.2008.05.094)

Main, C.A., Petersson, H.M., Rahman, S.S. and Hartley, R.C. (2008) High throughput synthesis of 2,5-substituted indoles using a titanium carbenoid bearing boronate functionality. Tetrahedron, 64(5), pp. 901-914. (doi:10.1016/j.tet.2007.08.119)

Mullen, W., Rouanet, J.-M., Auger, C., Teissèdre, P.-L., Caldwell, S.T., Hartley, R.C., Lean, M.E.J., Edwards, C.A. and Crozier, A. (2008) Bioavailability of [2-14C]Quercetin-4′-glucoside in rats. Journal of Agricultural and Food Chemistry, 56(24), pp. 12127-12137. (doi:10.1021/jf802754s)

2007

Caldwell, S. T., Quin, C., Edge, R. and Hartley, R. C. (2007) A dual sensor spin trap for use with EPR spectroscopy. Organic Letters, 9(18), pp. 3499-3502. (doi:10.1021/ol071285o)

Adriaenssens, L. V. and Hartley, R. C. (2007) beta-amino acids to piperidinones by petasis methylenation and acid-induced cyclization. Journal of Organic Chemistry, 72(26), pp. 10287-10290. (doi:10.1021/jo7019948)

Austin, C., Smith, D. and Hartley, R. (2007) Solid-phase synthesis of cyclic imines - potential for radiolabelling. Journal of Labelled Compounds and Radiopharmaceuticals, 50, pp. 502-503. (doi:10.1002/jlcr.1224)

Farrugia, L., Hartley, R., Calver, A. and Rahman, S. (2007) 5-Bromoanthranil. Acta Crystallographica. Section E: Structure Reports Online, 63, O2540-O2541. (doi:10.1107/S1600536807017278)

Hartley, R., Li, J., Main, C. and McKiernan, G. (2007) Titanium carbenoid reagents for converting carbonyl groups into alkenes. Tetrahedron, 63, pp. 4825-4864. (doi:10.1016/j.tet.2007.03.015)

2006

Adriaenssens, L., Austin, C., Gibson, M., Smith, D. and Hartley, R. (2006) Stereodivergent diversity oriented synthesis of piperidine alkaloids. European Journal of Organic Chemistry, pp. 4998-5001. (doi:10.1002/ejoc.200600744)

Caldwell, S., Petersson, H., Farrugia, L., Mullen, W., Crozier, A. and Hartley, R. (2006) Isotopic labelling of quercetin 3-glucoside. Tetrahedron, 62, pp. 7257-7265. (doi:10.1016/j.tet.2006.05.046)

Sklavounou, E., Hay, A., Ashraf, N., Lamb, K., Brown, E., Mac Intyre, A., George, W.D., Hartley, R.C. and Shiels, P.G. (2006) The use of telomere biology to identify and develop superior nitrone based anti-oxidants. Biochemical and Biophysical Research Communications, 347, pp. 420-427. (doi:10.1016/j.bbrc.2006.06.087)

2005

Graf, B., Mullen, W., Caldwell, S., Hartley, R., Duthie, G., Lean, M., Crozier, A. and Edwards, C. (2005) Disposition and metabolism of [2-C-14]quercetin-4 '-glucoside in rats. Drug Metabolism and Disposition, 33, pp. 1036-1043. (doi:10.1124/dmd.104.002691)

Hay, A., Burkitt, M., Jones, C. and Hartley, R. (2005) Development of a new EPR spin trap, DOD-8C (N-[4-dodecyloxy-2-(7'-carboxyhept-1'-yloxy) benzylidene]-N-tert-butylamine N-oxide), for the trapping of lipid radicals at a predetermined depth within biological membranes. Archives of Biochemistry and Biophysics, 435, pp. 336-346. (doi:10.1016/j.abb.2004.12.030)

2004

Bennett, C., Caldwell, S., McPhail, D., Morrice, P., Duthie, G. and Hartley, R. (2004) Potential therapeutic antioxidants that combine the radical scavenging ability of myricetin and the lipophilic chain of vitamin E to effectively inhibit microsomal lipid peroxidation. Bioorganic and Medicinal Chemistry, 12, pp. 2079-2098. (doi:10.1016/j.bmc.2004.02.031)

Macleod, C., Austin, C., Hamprecht, D. and Hartley, R. (2004) Preparation of quinolines from resin-bound esters using titanium reagents. Tetrahedron Letters, 45, pp. 8879-8882. (doi:10.1016/j.tetlet.2004.09.170)

Roberts, C. and Hartley, R. (2004) Titanium reagents for the synthesis of 2-substituted benzo[b]thiophenes on the solid phase. Journal of Organic Chemistry, 69, pp. 6145-6148. (doi:10.1021/jo049344o)

Woodland, C., Crawley, G. and Hartley, R. (2004) Stereoselectivity in the anionic oxy-Cope rearrangement of acyclic vinyl sulfides. Tetrahedron Letters, 45, pp. 1227-1231. (doi:10.1016/j.tetlet.2003.11.132)

2003

Gibson, M., Goodman, J., Farrugia, L. and Hartley, R. (2003) Controlling neighbouring group participation from thioacetals. Tetrahedron Letters, 44, pp. 2841-2844. (doi:10.1016/S0040-4039(03)00440-4)

Macleod, C., McKiernan, G., Guthrie, E., Farrugia, L., Hamprecht, D., Macritchie, J. and Hartley, R. (2003) Synthesis of 2-substituted benzofurans and Indoles using functionalized titanium benzylidene reagents on solid phase. Journal of Organic Chemistry, 68, pp. 387-401. (doi:10.1021/jo026384o)

McKiernan, G. and Hartley, R. (2003) Boronate titanium alkylidene reagents for diversity-based synthesis of benzofurans. Organic Letters, 5, pp. 4389-4392. (doi:10.1021/ol035677g)

McPhail, D., Hartley, R., Gardner, P. and Duthie, G. (2003) Kinetic and stoichiometric assessment of the antioxidant activity of flavonoids by electron spin resonance spectroscopy. Journal of Agricultural and Food Chemistry, 51, pp. 1684-1690. (doi:10.1021/jf025922v)

Mullen, W., Hartley, R. and Crozier, A. (2003) Detection and identification of C-14-labelled flavonol metabolites by high-performance liquid chromatography-radiocounting and tandem mass spectrometry. Journal of Chromatography A, 1007, pp. 21-29. (doi:10.1016/S0021-9673(03)00882-3)

2002

Hartley, R.C. and McKiernan, G.J. (2002) Titanium reagents for the alkylidenation of carboxylic acid and carbonic acid derivatives. Journal of the Chemical Society: Perkin Transactions 1, pp. 2763-2793. (doi:10.1039/b009709h)

Macleod, C., Hartley, R.C. and Hamprecht, D.W. (2002) Novel functionalized titanium(IV) benzylidenes for the traceless solid-phase synthesis of indoles. Organic Letters, 4, pp. 75-78. (doi:10.1021/ol016924o)

Mullen, W., Graf, B., Caldwell, S., Hartley, R., Duthie, G., Edwards, C., Lean, M. and Crozier, A. (2002) Determination of flavonol metabolites in plasma and tissues of rats by HPLC-radiocounting and tandem mass spectrometry following oral ingestion of [2-C-14]quercetin-4' glucoside. Journal of Agricultural and Food Chemistry, 50, pp. 6902-6909. (doi:10.1021/jf020598p)

2001

Rutherford, A.P., Gibb, C.S., Hartley, R.C. and Goodman, J.M. (2001) Stereocontrol in a one-pot procedure for anionic oxy-Cope rearrangement followed by intramolecular aldol reaction. Journal of the Chemical Society: Perkin Transactions 1, pp. 1051-1061. (doi:10.1039/b010138i)

This list was generated on Sat May 27 03:52:42 2017 BST.
Number of items: 57.

Articles

Arndt, S. et al. (2017) Assessment of H2S in vivo using the newly developed mitochondria-targeted mass spectrometry probe MitoA. Journal of Biological Chemistry, (doi:10.1074/jbc.M117.784678) (Early Online Publication)

Salin, K., Auer, S. K., Villasevil, E. M., Anderson, G. J., Cairns, A. G., Mullen, W. , Hartley, R. C. and Metcalfe, N. B. (2017) Using the MitoB method to assess levels of reactive oxygen species in ecological studies of oxidative stress. Scientific Reports, 7, 41228. (doi:10.1038/srep41228)

Buskiewicz, I. A. et al. (2016) Reactive oxygen species induce virus-independent MAVS-oligomerization in systemic lupus erythematosus. Science Signaling, 9(456), ra115. (doi:10.1126/scisignal.aaf1933) (PMID:27899525)

McGarry, D. J., Shchepinova, M. M., Lillla, S., Hartley, R. C. and Olson, M. F. (2016) A cell-permeable biscyclooctyne as a novel probe for the identification of protein sulfenic acids. ACS Chemical Biology, 11(12), pp. 3300-3304. (doi:10.1021/acschembio.6b00742) (PMID:27792307)

Caldwell, S. T., Cairns, A. G., Olson, M., Chalmers, S., Sandison, M., Mullen, W., McCarron, J. G. and Hartley, R. C. (2015) Synthesis of an azido-tagged low affinity ratiometric calcium sensor. Tetrahedron, 71(51), pp. 9571-9578. (doi:10.1016/j.tet.2015.10.052) (PMID:26709317) (PMCID:PMC4660056)

Robb, E. L. et al. (2015) Selective superoxide generation within mitochondria by the targeted redox cycler MitoParaquat. Free Radical Biology and Medicine, 89, pp. 883-894. (doi:10.1016/j.freeradbiomed.2015.08.021) (PMID:26454075)

Salin, K., Auer, S. k., Rudolf, A. M., Anderson, G. J., Cairns, A. G., Mullen, W., Hartley, R. C. , Selman, C. and Metcalfe, N. B. (2015) Individuals with higher metabolic rates have lower levels of reactive oxygen species in vivo. Biology Letters, 11(9), 20150538. (doi:10.1098/rsbl.2015.0538) (PMID:26382073)

Chouchani, E. T. et al. (2014) Ischaemic accumulation of succinate controls reperfusion injury through mitochondrial ROS. Nature, 515(7527), pp. 431-435. (doi:10.1038/nature13909) (PMID:25383517) (PMCID:PMC4255242)

Logan, A., Shabalina, I. G., Prime, T. A., Rogatti, S., Kalinovich, A. V., Hartley, R. C., Budd, R. C., Cannon, B. and Murphy, M. P. (2014) In vivo levels of mitochondrial hydrogen peroxide increase with age in mtDNA mutator mice. Aging Cell, 13(4), pp. 765-768. (doi:10.1111/acel.12212) (PMID:24621297) (PMCID:PMC4326952)

Cairns, A. G., Senn, H. M., Murphy, M. P. and Hartley, R. C. (2014) Expanding the palette of phenanthridinium cations. Chemistry: A European Journal, 20(13), pp. 3742-3751. (doi:10.1002/chem.201304241) (PMID:24677631) (PMCID:PMC4164275)

Li Pun, P. B. et al. (2014) A mitochondria-targeted mass spectrometry probe to detect glyoxals: implications for diabetes. Free Radical Biology and Medicine, 67, pp. 437-450. (doi:10.1016/j.freeradbiomed.2013.11.025) (PMID:24316194) (PMCID:PMC3978666)

Logan, A. et al. (2014) Using exomarkers to assess mitochondrial reactive species in vivo. Biochimica et Biophysica Acta: General Subjects, 1840(2), pp. 923-930. (doi:10.1016/j.bbagen.2013.05.026) (PMID:23726990)

McQuaker, S. J., Quinlan, C. L., Caldwell, S. T., Brand, M. D. and Hartley, R. C. (2013) A prototypical small-molecule modulator uncouples mitochondria in response to endogenous hydrogen peroxide production. ChemBioChem, 14(8), pp. 993-1000. (doi:10.1002/cbic.201300115) (PMID:23640856) (PMCID:PMC3743171)

Chouchani, E. T. et al. (2013) Cardioprotection by S-nitrosation of a cysteine switch on mitochondrial complex I. Nature Medicine, 19(6), pp. 753-759. (doi:10.1038/nm.3212) (PMID:23708290) (PMCID:PMC4019998)

Sikora, A., Zielonka, J., Adamus, J., Debski, D., Dybala-Defratyka, A., Michalowski, B., Joseph, J., Hartley, R.C., Murphy, M.P. and Kalyanaraman, B. (2013) Reaction between peroxynitrite and triphenylphosphonium-substituted arylboronic acid isomers: identification of diagnostic marker products and biological implications. Chemical Research in Toxicology, 26(6), pp. 856-867. (doi:10.1021/tx300499c) (PMID:23611338) (PMCID:PMC3700382)

Harvey, A. L., Young, L. C., Kornisiuk, E., Snitcofsky, M., Colettis, N., Blanco, C., Jerusalinsky, D., Jamieson, A. G. , Hartley, R. C. and Stone, T. W. (2012) A novel dihydro-pyrazolo(3,4d)(1,2,4)triazolo(1,5a)pyrimidin-4-one (AJ23) is an antagonist at adenosine A1 receptors and enhances consolidation of step-down avoidance. Behavioural Brain Research, 234(2), pp. 184-191. (doi:10.1016/j.bbr.2012.06.023)

Smith, R.A.J., Hartley, R.C., Cochemé, H.M. and Murphy, M.P. (2012) Mitochondrial pharmacology. Trends in Pharmacological Sciences, 33(6), pp. 341-352. (doi:10.1016/j.tips.2012.03.010) (PMID:22521106)

Cochemé, H.M. et al. (2012) Using the mitochondria-targeted ratiometric mass spectrometry probe MitoB to measure H2O2 in living Drosophila. Nature Protocols, 7(5), pp. 946-958. (doi:10.1038/nprot.2012.035) (PMID:22517261)

Caldwell, S.T., McPhail, D.B., Duthie, G.D. and Hartley, R.C. (2012) Synthesis of polyhydroxylated flavonoids bearing a lipophilic decyl tail as potential therapeutic antioxidants. Canadian Journal of Chemistry = Journal canadien de chimie, 90(1), pp. 23-33. (doi:10.1139/V11-087)

Chalmers, S., Caldwell, S. T., Quin, C., Prime, T. A., James, A. M., Cairns, A. G., Murphy, M. P., McCarron, J. G. and Hartley, R. C. (2012) Selective uncoupling of individual mitochondria within a cell using a mitochondria-targeted photoactivated protonophore. Journal of the American Chemical Society, 134(2), pp. 758-761. (doi:10.1021/ja2077922) (PMID:22239373) (PMCID:PMC3260739)

Omar, M.H., Mullen, W., Stalmach, A., Auger, C., Rouanet, J.-M., Teissedre, P.-L., Caldwell, S.T., Hartley, R.C. and Crozier, A. (2012) Absorption, disposition, metabolism, and excretion of [3-14C]caffeic acid in rats. Journal of Agricultural and Food Chemistry, 60(20), pp. 5205-5214. (doi:10.1021/jf3001185)

Smith, R. A.J., Hartley, R. C. and Murphy, M. P. (2011) Mitochondria-targeted small molecule therapeutics and probes. Antioxidants and Redox Signaling, 15(12), pp. 3021-3038. (doi:10.1089/ars.2011.3969) (PMID:21395490)

Figlus, M., Wellaway, N., Cooper, A.W.J., Sollis, S.L. and Hartley, R.C. (2011) Synthesis of arrays using low molecular weight MPEG-assisted Mitsunobu reaction. ACS Combinatorial Science, 13(3), pp. 280-285. (doi:10.1021/co100091n)

Cochemé, H.M. et al. (2011) Measurement of H2O2 within living drosophila during aging using a ratiometric mass spectrometry probe targeted to the mitochondrial matrix. Cell Metabolism, 13(3), pp. 340-350. (doi:10.1016/j.cmet.2011.02.003) (PMID:21356523) (PMCID:PMC4413513)

Haahr, A., Rankovic, Z. and Hartley, R. (2011) A one-pot procedure for methylenating carbonyl compounds using the Nysted reagent and titanocene dichloride. Tetrahedron Letters, 52(23), pp. 3020-3022. (doi:10.1016/j.tetlet.2011.04.017)

Quin, C., Robertson, L., McQuaker, S.J., Price, N.C., Brand, M.D. and Hartley, R.C. (2010) Caged mitochondrial uncouplers that are released in response to hydrogen peroxide. Tetrahedron, 66(13), pp. 2384-2389. (doi:10.1016/j.tet.2010.01.103) (PMID:20418941) (PMCID:PMC2852674)

Figlus, M., Tarruella, A.C., Messer, A., Sollis, S.L. and Hartley, R.C. (2010) Low molecular weight MPEG-assisted organic synthesis. Chemical Communications, 46(24), pp. 4405-4407. (doi:10.1039/c0cc00415d)

Quin, C., Trnka, J., Hay, A., Murphy, M.P. and Hartley, R.C. (2009) Synthesis of a mitochondria-targeted spin trap using a novel Parham-type cyclization. Tetrahedron, 65(39), pp. 8154-8160. (doi:10.1016/j.tet.2009.07.081) (PMID:19888470) (PMCID:PMC2767131)

Robertson, L. and Hartley, R. C. (2009) Synthesis of N-arylpyridinium salts bearing a nitrone spin trap as potential mitochondria-targeted antioxidants. Tetrahedron, 65(27), pp. 5284-5292. (doi:10.1016/j.tet.2009.04.083) (PMID:19693262) (PMCID:PMC2722452)

El Fangour, S., Marini, M., Good, J., McQuaker, S.J., Shiels, P.G. and Hartley, R.C. (2009) Nitrones for understanding and ameliorating the oxidative stress associated with aging. Age, 31(4), pp. 269-276. (doi:10.1007/s11357-009-9098-z) (PMID:19479343) (PMCID:PMC2813050)

Main, C.A., Rahman, S.S. and Hartley, R.C. (2008) Synthesis of spiroacetals using functionalised titanium carbenoids. Tetrahedron Letters, 49(32), pp. 4771-4774. (doi:10.1016/j.tetlet.2008.05.094)

Main, C.A., Petersson, H.M., Rahman, S.S. and Hartley, R.C. (2008) High throughput synthesis of 2,5-substituted indoles using a titanium carbenoid bearing boronate functionality. Tetrahedron, 64(5), pp. 901-914. (doi:10.1016/j.tet.2007.08.119)

Mullen, W., Rouanet, J.-M., Auger, C., Teissèdre, P.-L., Caldwell, S.T., Hartley, R.C., Lean, M.E.J., Edwards, C.A. and Crozier, A. (2008) Bioavailability of [2-14C]Quercetin-4′-glucoside in rats. Journal of Agricultural and Food Chemistry, 56(24), pp. 12127-12137. (doi:10.1021/jf802754s)

Caldwell, S. T., Quin, C., Edge, R. and Hartley, R. C. (2007) A dual sensor spin trap for use with EPR spectroscopy. Organic Letters, 9(18), pp. 3499-3502. (doi:10.1021/ol071285o)

Adriaenssens, L. V. and Hartley, R. C. (2007) beta-amino acids to piperidinones by petasis methylenation and acid-induced cyclization. Journal of Organic Chemistry, 72(26), pp. 10287-10290. (doi:10.1021/jo7019948)

Austin, C., Smith, D. and Hartley, R. (2007) Solid-phase synthesis of cyclic imines - potential for radiolabelling. Journal of Labelled Compounds and Radiopharmaceuticals, 50, pp. 502-503. (doi:10.1002/jlcr.1224)

Farrugia, L., Hartley, R., Calver, A. and Rahman, S. (2007) 5-Bromoanthranil. Acta Crystallographica. Section E: Structure Reports Online, 63, O2540-O2541. (doi:10.1107/S1600536807017278)

Hartley, R., Li, J., Main, C. and McKiernan, G. (2007) Titanium carbenoid reagents for converting carbonyl groups into alkenes. Tetrahedron, 63, pp. 4825-4864. (doi:10.1016/j.tet.2007.03.015)

Adriaenssens, L., Austin, C., Gibson, M., Smith, D. and Hartley, R. (2006) Stereodivergent diversity oriented synthesis of piperidine alkaloids. European Journal of Organic Chemistry, pp. 4998-5001. (doi:10.1002/ejoc.200600744)

Caldwell, S., Petersson, H., Farrugia, L., Mullen, W., Crozier, A. and Hartley, R. (2006) Isotopic labelling of quercetin 3-glucoside. Tetrahedron, 62, pp. 7257-7265. (doi:10.1016/j.tet.2006.05.046)

Sklavounou, E., Hay, A., Ashraf, N., Lamb, K., Brown, E., Mac Intyre, A., George, W.D., Hartley, R.C. and Shiels, P.G. (2006) The use of telomere biology to identify and develop superior nitrone based anti-oxidants. Biochemical and Biophysical Research Communications, 347, pp. 420-427. (doi:10.1016/j.bbrc.2006.06.087)

Graf, B., Mullen, W., Caldwell, S., Hartley, R., Duthie, G., Lean, M., Crozier, A. and Edwards, C. (2005) Disposition and metabolism of [2-C-14]quercetin-4 '-glucoside in rats. Drug Metabolism and Disposition, 33, pp. 1036-1043. (doi:10.1124/dmd.104.002691)

Hay, A., Burkitt, M., Jones, C. and Hartley, R. (2005) Development of a new EPR spin trap, DOD-8C (N-[4-dodecyloxy-2-(7'-carboxyhept-1'-yloxy) benzylidene]-N-tert-butylamine N-oxide), for the trapping of lipid radicals at a predetermined depth within biological membranes. Archives of Biochemistry and Biophysics, 435, pp. 336-346. (doi:10.1016/j.abb.2004.12.030)

Bennett, C., Caldwell, S., McPhail, D., Morrice, P., Duthie, G. and Hartley, R. (2004) Potential therapeutic antioxidants that combine the radical scavenging ability of myricetin and the lipophilic chain of vitamin E to effectively inhibit microsomal lipid peroxidation. Bioorganic and Medicinal Chemistry, 12, pp. 2079-2098. (doi:10.1016/j.bmc.2004.02.031)

Macleod, C., Austin, C., Hamprecht, D. and Hartley, R. (2004) Preparation of quinolines from resin-bound esters using titanium reagents. Tetrahedron Letters, 45, pp. 8879-8882. (doi:10.1016/j.tetlet.2004.09.170)

Roberts, C. and Hartley, R. (2004) Titanium reagents for the synthesis of 2-substituted benzo[b]thiophenes on the solid phase. Journal of Organic Chemistry, 69, pp. 6145-6148. (doi:10.1021/jo049344o)

Woodland, C., Crawley, G. and Hartley, R. (2004) Stereoselectivity in the anionic oxy-Cope rearrangement of acyclic vinyl sulfides. Tetrahedron Letters, 45, pp. 1227-1231. (doi:10.1016/j.tetlet.2003.11.132)

Gibson, M., Goodman, J., Farrugia, L. and Hartley, R. (2003) Controlling neighbouring group participation from thioacetals. Tetrahedron Letters, 44, pp. 2841-2844. (doi:10.1016/S0040-4039(03)00440-4)

Macleod, C., McKiernan, G., Guthrie, E., Farrugia, L., Hamprecht, D., Macritchie, J. and Hartley, R. (2003) Synthesis of 2-substituted benzofurans and Indoles using functionalized titanium benzylidene reagents on solid phase. Journal of Organic Chemistry, 68, pp. 387-401. (doi:10.1021/jo026384o)

McKiernan, G. and Hartley, R. (2003) Boronate titanium alkylidene reagents for diversity-based synthesis of benzofurans. Organic Letters, 5, pp. 4389-4392. (doi:10.1021/ol035677g)

McPhail, D., Hartley, R., Gardner, P. and Duthie, G. (2003) Kinetic and stoichiometric assessment of the antioxidant activity of flavonoids by electron spin resonance spectroscopy. Journal of Agricultural and Food Chemistry, 51, pp. 1684-1690. (doi:10.1021/jf025922v)

Mullen, W., Hartley, R. and Crozier, A. (2003) Detection and identification of C-14-labelled flavonol metabolites by high-performance liquid chromatography-radiocounting and tandem mass spectrometry. Journal of Chromatography A, 1007, pp. 21-29. (doi:10.1016/S0021-9673(03)00882-3)

Hartley, R.C. and McKiernan, G.J. (2002) Titanium reagents for the alkylidenation of carboxylic acid and carbonic acid derivatives. Journal of the Chemical Society: Perkin Transactions 1, pp. 2763-2793. (doi:10.1039/b009709h)

Macleod, C., Hartley, R.C. and Hamprecht, D.W. (2002) Novel functionalized titanium(IV) benzylidenes for the traceless solid-phase synthesis of indoles. Organic Letters, 4, pp. 75-78. (doi:10.1021/ol016924o)

Mullen, W., Graf, B., Caldwell, S., Hartley, R., Duthie, G., Edwards, C., Lean, M. and Crozier, A. (2002) Determination of flavonol metabolites in plasma and tissues of rats by HPLC-radiocounting and tandem mass spectrometry following oral ingestion of [2-C-14]quercetin-4' glucoside. Journal of Agricultural and Food Chemistry, 50, pp. 6902-6909. (doi:10.1021/jf020598p)

Rutherford, A.P., Gibb, C.S., Hartley, R.C. and Goodman, J.M. (2001) Stereocontrol in a one-pot procedure for anionic oxy-Cope rearrangement followed by intramolecular aldol reaction. Journal of the Chemical Society: Perkin Transactions 1, pp. 1051-1061. (doi:10.1039/b010138i)

Book Sections

Cairns, A. G., McQuaker, S. J., Murphy, M. P. and Hartley, R. C. (2015) Targeting mitochondria with small molecules: the preparation of MitoB and MitoP as exomarkers of mitochondrial hydrogen peroxide. In: Weissig, V. and Edeas, M. (eds.) Mitochondrial Medicine: Volume II, Manipulating Mitochondrial Function. Series: Methods in Molecular Biology, 1265. Springer New York, pp. 25-50. ISBN 9781493922871 (doi:10.1007/978-1-4939-2288-8_3)

This list was generated on Sat May 27 03:52:42 2017 BST.