Dr Christopher Syme

  • Research Associate (Centre for Virus Research)
  • Honorary Research Associate (School of Chemistry)

Biography

My research interests include the development of novel spectroscopic and imaging techniques, with a particular emphasis on Raman spectroscopy and associated techniques such as Raman optical activity (ROA), SERRS and CARS microscopy. Raman-based techniques yield a wealth of chemical and structural information without the need for labelling or modification of the sample. Raman data can be obtained non-destructively enabling studies on a vast array of samples and substrates including live cells and tissues. ROA is exquisitely sensitive to molecular chirality and is a powerful probe of biomolecular structure. Many of the most important molecules in living systems are chiral, such as amino acids, peptides and proteins, nucleic acids (DNA and RNA), viral capsids and amyloid fibrillar species that are implicated in a range of neurodegenerative illnesses such as Alzheimer’s disease. Recent advances in technology have paved the way for the rapid development of increasingly powerful Raman-based techniques, such as coherent anti-Stokes Raman scattering (CARS) microscopy. CARS boosts the Raman signal such that images may be collected in a fraction of the time, enabling high-resolution video-rate imaging, and opening upon a wide range of new possibilities.

 


Publications

List by: Type | Date

Jump to: 2017 | 2016 | 2015 | 2013 | 2012 | 2011 | 2010 | 2003 | 2002 | 2001
Number of items: 16.

2017

Syme, C. D., Mosses, J., González-Jiménez, M., Shebanova, O., Walton, F. and Wynne, K. (2017) Frustration of crystallisation by a liquid–crystal phase. Scientific Reports, 7, 42439. (doi:10.1038/srep42439) (PMID:28209972) (PMCID:PMC5314399)

2016

Fast, A., Kenison, J. P., Syme, C. D. and Potma, E. O. (2016) Surface-enhanced coherent anti-Stokes Raman imaging of lipids. Applied Optics, 55(22), pp. 5994-6000. (doi:10.1364/AO.55.005994) (PMID:27505381)

2015

Mosses, J., Syme, C. D. and Wynne, K. (2015) The order parameter of the liquid-liquid transition in a molecular liquid. Journal of Physical Chemistry Letters, 6(1), pp. 38-43. (doi:10.1021/jz5022763)

2013

Kadodwala, M., Abdulrahman, N., Syme, C. D., Jack, C., Barron, L. D., Karimullah, A. and Gadegaard, N. (2013) The origin of off–resonance non-linear optical activity of a gold chiral nanomaterial. Nanoscale, 5(24), pp. 12651-12657. (doi:10.1039/c3nr04746f)

Reboud, J., Auchinvole, C., Syme, C.D., Wilson, R. and Cooper, J.M. (2013) Acoustically controlled enhancement of molecular sensing to assess oxidative stress in cells. Chemical Communications, 49(28), pp. 2918-2920. (doi:10.1039/C3CC37931K)

2012

Sirimuthu, N.M.S., Syme, C.D. and Cooper, J.M. (2012) Intracellular multiplex detection and imaging of stable chemisorbed labels by SERS spectroscopy. Proceedings of the SPIE: The International Society for Optical Engineering, 8234, 82340F. (doi:10.1117/12.906419)

Syme, C.D., Martino, C., Yusvana, R., Sirimuthu, N.M.S. and Cooper, J.M. (2012) Quantitative characterization of individual microdroplets using surface-enhanced resonance Raman scattering spectroscopy. Analytical Chemistry, 84(3), pp. 1491-1495. (doi:10.1021/ac202705a)

Syme, C.D., Sirimuthu, N., Martino, C., Yusvana, R. and Cooper, J.M. (2012) Characterization of individual microdroplets by SERRS spectroscopy. Proceedings of the SPIE: The International Society for Optical Engineering, 8234, 82340I. (doi:10.1117/12.906374)

2011

Sirimuthu, N., Syme, C. and Cooper, J. (2011) Investigation of the stability of labelled nanoparticles for SE(R)RS measurements in cells. Chemical Communications, 47(14), pp. 4099-4101. (doi:10.1039/c0cc05723a)

2010

Sirimuthu, N.M.S., Syme, C.D. and Cooper, J.M. (2010) Monitoring the uptake and redistribution of metal nanoparticles during cell culture using surface-enhanced raman scattering spectroscopy. Analytical Chemistry, 82(17), pp. 7369-7373. (doi:10.1021/ac101480t)

Syme, C.D., Sirimuthu, N.M.S., Faley, S.L. and Cooper, J. (2010) SERS mapping of nanoparticle labels in single cells using a microfluidic chip. Chemical Communications, 46(42), pp. 7921-7923. (doi:10.1039/C0CC02209H)

2003

Barron, L.D., Blanch, E.W., McColl, I.H., Syme, C.D., Hecht, L. and Nielsen, K. (2003) Structure and behaviour of proteins, nucleic acids and viruses from vibrational Raman optical activity. Spectroscopy, 17(2-3), pp. 101-126. (doi:10.1155/2003/787940)

2002

Blanch, E.W., Robinson, D.J., Hecht, L., Syme, C.D., Nielsen, K. and Barron, L.D. (2002) Solution structures of potato virus X and narcissus mosaic virus from Raman optical activity. Journal of General Virology, 83(1), pp. 241-246.

Syme, C.D., Blanch, E.W., Holt, C., Jakes, R., Goedert, M., Hecht, L. and Barron, L.D. (2002) A Raman optical activity study of rheomorphism in caseins, synucleins and tau - New insight into the structure and behaviour of natively unfolded proteins. European Journal of Biochemistry, 269(1), pp. 148-156. (doi:10.1046/j.0014-2956.2001.02633.x)

Blanch, E.W., Hecht, L., Syme, C.D., Volpetti, V., Lomonossoff, G.P., Nielsen, K. and Barron, L.D. (2002) Molecular structures of viruses from Raman optical activity. Journal of General Virology, 83(10), pp. 2593-2600.

2001

Smyth, E., Syme, C.D., Blanch, E.W., Hecht, L., Vasak, M. and Barron, L.D. (2001) Solution structure of native proteins with irregular folds from Raman optical activity. Biopolymers, 58(2), pp. 138-151. (doi:10.1002/1097-0282(200102)58:2<138::AID-BIP30>3.0.CO;2-W)

This list was generated on Tue May 23 04:48:49 2017 BST.
Jump to: Articles
Number of items: 16.

Articles

Syme, C. D., Mosses, J., González-Jiménez, M., Shebanova, O., Walton, F. and Wynne, K. (2017) Frustration of crystallisation by a liquid–crystal phase. Scientific Reports, 7, 42439. (doi:10.1038/srep42439) (PMID:28209972) (PMCID:PMC5314399)

Fast, A., Kenison, J. P., Syme, C. D. and Potma, E. O. (2016) Surface-enhanced coherent anti-Stokes Raman imaging of lipids. Applied Optics, 55(22), pp. 5994-6000. (doi:10.1364/AO.55.005994) (PMID:27505381)

Mosses, J., Syme, C. D. and Wynne, K. (2015) The order parameter of the liquid-liquid transition in a molecular liquid. Journal of Physical Chemistry Letters, 6(1), pp. 38-43. (doi:10.1021/jz5022763)

Kadodwala, M., Abdulrahman, N., Syme, C. D., Jack, C., Barron, L. D., Karimullah, A. and Gadegaard, N. (2013) The origin of off–resonance non-linear optical activity of a gold chiral nanomaterial. Nanoscale, 5(24), pp. 12651-12657. (doi:10.1039/c3nr04746f)

Reboud, J., Auchinvole, C., Syme, C.D., Wilson, R. and Cooper, J.M. (2013) Acoustically controlled enhancement of molecular sensing to assess oxidative stress in cells. Chemical Communications, 49(28), pp. 2918-2920. (doi:10.1039/C3CC37931K)

Sirimuthu, N.M.S., Syme, C.D. and Cooper, J.M. (2012) Intracellular multiplex detection and imaging of stable chemisorbed labels by SERS spectroscopy. Proceedings of the SPIE: The International Society for Optical Engineering, 8234, 82340F. (doi:10.1117/12.906419)

Syme, C.D., Martino, C., Yusvana, R., Sirimuthu, N.M.S. and Cooper, J.M. (2012) Quantitative characterization of individual microdroplets using surface-enhanced resonance Raman scattering spectroscopy. Analytical Chemistry, 84(3), pp. 1491-1495. (doi:10.1021/ac202705a)

Syme, C.D., Sirimuthu, N., Martino, C., Yusvana, R. and Cooper, J.M. (2012) Characterization of individual microdroplets by SERRS spectroscopy. Proceedings of the SPIE: The International Society for Optical Engineering, 8234, 82340I. (doi:10.1117/12.906374)

Sirimuthu, N., Syme, C. and Cooper, J. (2011) Investigation of the stability of labelled nanoparticles for SE(R)RS measurements in cells. Chemical Communications, 47(14), pp. 4099-4101. (doi:10.1039/c0cc05723a)

Sirimuthu, N.M.S., Syme, C.D. and Cooper, J.M. (2010) Monitoring the uptake and redistribution of metal nanoparticles during cell culture using surface-enhanced raman scattering spectroscopy. Analytical Chemistry, 82(17), pp. 7369-7373. (doi:10.1021/ac101480t)

Syme, C.D., Sirimuthu, N.M.S., Faley, S.L. and Cooper, J. (2010) SERS mapping of nanoparticle labels in single cells using a microfluidic chip. Chemical Communications, 46(42), pp. 7921-7923. (doi:10.1039/C0CC02209H)

Barron, L.D., Blanch, E.W., McColl, I.H., Syme, C.D., Hecht, L. and Nielsen, K. (2003) Structure and behaviour of proteins, nucleic acids and viruses from vibrational Raman optical activity. Spectroscopy, 17(2-3), pp. 101-126. (doi:10.1155/2003/787940)

Blanch, E.W., Robinson, D.J., Hecht, L., Syme, C.D., Nielsen, K. and Barron, L.D. (2002) Solution structures of potato virus X and narcissus mosaic virus from Raman optical activity. Journal of General Virology, 83(1), pp. 241-246.

Syme, C.D., Blanch, E.W., Holt, C., Jakes, R., Goedert, M., Hecht, L. and Barron, L.D. (2002) A Raman optical activity study of rheomorphism in caseins, synucleins and tau - New insight into the structure and behaviour of natively unfolded proteins. European Journal of Biochemistry, 269(1), pp. 148-156. (doi:10.1046/j.0014-2956.2001.02633.x)

Blanch, E.W., Hecht, L., Syme, C.D., Volpetti, V., Lomonossoff, G.P., Nielsen, K. and Barron, L.D. (2002) Molecular structures of viruses from Raman optical activity. Journal of General Virology, 83(10), pp. 2593-2600.

Smyth, E., Syme, C.D., Blanch, E.W., Hecht, L., Vasak, M. and Barron, L.D. (2001) Solution structure of native proteins with irregular folds from Raman optical activity. Biopolymers, 58(2), pp. 138-151. (doi:10.1002/1097-0282(200102)58:2<138::AID-BIP30>3.0.CO;2-W)

This list was generated on Tue May 23 04:48:49 2017 BST.