Dr Adrian Lapthorn

  • Senior Lecturer (Chemistry)

telephone: 01413305940
email: Adrian.Lapthorn@glasgow.ac.uk

Research interests

Research Interests

My research interests are in using the technique of protein crystallography to elucidate protein structure with an aim to answering important questions on protein function and mechanism. I also have a specific interest in protein-protein interactions. Protein Structure and Function; Enzymes of the Shikimate pathway; Structure aided drug discovery; Protein Crystallography; Plant Glutathione S-transferases.

More details are available on the personal Research Pages.

Research Topics

Protein Structure and Function
Enzymes of the Shikimate pathway
Structure aided drug discovery
Protein Crystallography
Plant Glutathione S-transferases

Publication Highlights

"Binding and glutathione conjugation of porphyrinogens by plant glutathione transferases". Dixon, D.P., Lapthorn, A.J., Madesis, P., Mudd, E.A., Day, A. and Edwards, R. J. Biol. Chem. 283, 20268-20276 (2008)

"Design, synthesis, and structural studies on potent biaryl inhibitors of type II dehydroquinases". Payne, R.J., Riboldi-Tunnicliffe, A., Kerbarh, O., Abell, A.D., Lapthorn, A.J. and Abell, C. ChemMedChem, 2, 1010-1013 (2007)

"Crystal structures of Helicobacter pylori type II dehydroquinase inhibitor complexes: New directions for inhibitor design". D.A. Robinson, K.A. Stewart, N.C. Price, P.A. Chalk, J.R. Coggins, A.J. Lapthorn, J. Med. Chem., 49, 1282-1290 (2006)

"Crystal structure of mouse succinic semialdehyde reductase AKR7A5: Structural basis for substrate specificity". X.F. Zhu, A.J. Lapthorn, E.M. Ellis, Biochemistry, 45, 1562-1570 (2006)

"The structure of Escherichia coli ATP-phosphoribosyltransferase: Identification of substrate binding sites and mode of AMP inhibition". B. Lohkamp, G. McDermott, S.A. Campbell, J.R. Coggins, A.J. Lapthorn, J. Mol. Biol., 336, 131-144 (2004)

 

 

Teaching

Teaching

Science Fundamentals:- Aqueous Solutions Science Fundamentals:- Reaction Kinetics
Level-1 Chemistry:- Attractions and Repulsions
Level-3 Chemistry:- Biophysical Chemistry

Additional information

Awards

PhD (York)

 

Publications

List by: Type | Date

Jump to: 2014 | 2013 | 2011 | 2010 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002 | 2001
Number of items: 30.

2014

Turton, D. A., Senn, H., Harwood, T., Lapthorn, A. J., Ellis, E. M., and Wynne, K. (2014) Terahertz underdamped vibrational motion governs protein-ligand binding in solution. Nature Communications, 5, p. 3999. (doi:10.1038/ncomms4999)

2013

Turton, D., Harwood, T., Lapthorn, A., Ellis, E., and Wynne, K. (2013) Ultrabroadband terahertz spectroscopies of biomolecules and water. Proceedings of SPIE, 8623(862303), (doi:10.1117/12.2003796)

2011

Paz, S. et al. (2011) Tetrahydrobenzothiophene derivatives: conformationally restricted inhibitors of type II dehydroquinase. ChemMedChem, 6(2), pp. 266-272. (doi:10.1002/cmdc.201000343)

Tran, A.T., Cergol, K.M. , West, N.P. , Randall, E.J. , Britton, W.J. , Bokhari, S.A.I. , Ibrahim, M. , Lapthorn, A.J., and Payne, R.J. (2011) Synthesis and evaluation of potent ene-yne inhibitors of type II dehydroquinases as tuberculosis drug leads. ChemMedChem, 6(2), pp. 262-265. (doi:10.1002/cmdc.201000399)

2010

Hendry, E., Carpy, T., Johnston, J., Popland, M., Mikhaylovskiy, R.V., Lapthorn, A., Kelly, S.M., Barron, L.D., Gadegaard, N., and Kadodwala, M. (2010) Ultrasensitive detection and characterization of biomolecules using superchiral fields. Nature Nanotechnology, 5(11), pp. 783-787. (doi:10.1038/nnano.2010.209 )

Tran, A.T., Cergol, K.M., Britton, W.J., Bokhari, S.A.I., Ibrahim, M., Lapthorn, A.J., and Payne, R.J. (2010) Rapid assembly of potent type II dehydroquinase inhibitors via "Click" chemistry. MedChemComm, 1(4), pp. 271-275. (doi:10.1039/c0md00097c )

2008

Dixon, D. P., Lapthorn, A., Madesis, P., Mudd, E. A., Day, A., and Edwards, R. (2008) Binding and glutathione conjugation of porphyrinogens by plant glutathione transferases. Journal of Biological Chemistry, 283(29), pp. 20268-20276. (doi:10.1074/jbc.M802026200 )

Stewart, K. A., Robinson, D. A., and Lapthorn, A. J. (2008) Type II dehydroquinase: molecular replacement with many copies. Acta Crystallographica. Section D: Biological Crystallography, 64, pp. 108-118. (doi:10.1107/S0907444907054923 )

2007

Payne, R.J., Riboldi-Tunnicliffe, A., Kerbarh, O., Abell, A.D., Lapthorn, A.J., and Abell, C. (2007) Design, synthesis, and structural studies on potent biaryl inhibitors of type II dehydroquinases. ChemMedChem, 2(7), pp. 1010-1013. (doi:10.1002/cmdc.200700062)

2006

Lohkamp, B., Paterson, N., and Lapthorn, A. (2006) Application of a new vector system for efficient protein purification in the crystallization of PA5104/ORF from Pseudomonas aeruginosa. Journal of Structural Biology, 155, pp. 111-113. (doi:10.1016/j.jsb.2006.03.016)

Robinson, D.A.,, Stewart, K.A., Price, N.C., Chalk, P.A., Coggins, J.R., and Lapthorn, A.J. (2006) Crystal structures of Helicobacter pylori type II dehydroquinase inhibitor complexes: new directions for inhibitor design. Journal of Medicinal Chemistry, 49(4), pp. 1282-1290. (doi:10.1021/jm0505361)

Zhu, X., Lapthorn, A., and Ellis, E. (2006) Crystal structure of mouse succinic semialdehyde reductase AKR7A5: Structural basis for substrate specificity. Biochemistry, 45, pp. 1562-1570. (doi:10.1021/bi051610k)

2005

Dias-Gunasekara, S., Gubbens, J., van Lith, M., Dunne, C., Williams, J.A.G., Kataky, R., Scoones, D., Lapthorn, A., Bulleid, N.J., and Benham, A.M. (2005) Tissue-specific expression and dimerization of the endoplasmic reticulum oxidoreductase Ero1β. Journal of Biological Chemistry, 280, pp. 33066-33075. (doi:10.1074/jbc.M505023200)

Charrel-Dennis, M. et al. (2005) The human chorionic gonadotropin-beta arginine(68) to glutamic acid substitution fixes the conformation of the C-terminal peptide. Molecular Endocrinology, 19, pp. 1803-1811. (doi:10.1210/me.2004-0109)

Dixon, D., Lapthorn, A., and Edwards, R. (2005) Synthesis and analysis of chimeric maize glutathione transferases. Plant Science, 168, pp. 873-881. (doi:10.1016/j.plantsci.2004.11.009)

Toscano, M.D., Stewart, K.A., Coggins, J.R., Lapthorn, A.J., and Abell, C. (2005) Rational design of new bifunctional inhibitors of type II dehydroquinase. Organic and Biomolecular Chemistry, 3, pp. 3102-3104. (doi:10.1039/B507156A)

2004

Lohkamp, B., McDermott, G., Campbell, S.A., Coggins, J.R., and Lapthorn, A.J. (2004) The structure of Escherichia coli ATP-phosphoribosyltransferase: identification of substrate binding sites and mode of AMP inhibition. Journal of Molecular Biology, 336(1), pp. 131-144. (doi:10.1016/j.jmb.2003.12.020)

Charrel-Dennis, M., Jackson, A., Lund, T., Lapthorn, A., Berger, P., Roitt, I., and Delves, P. (2004) The major hormone-specific discontinuous epitopes on human chorionic gonadotrophin. Journal of Molecular Endocrinology, 32, pp. 571-581.

2003

Coggins, J.R., Abell, C., Evan, L.B., Frederickson, M., Robinson, D.A., Roszak, A.W., and Lapthorn, A.P. (2003) Experiences with the Shikimate-pathway enzymes as targets for rational drug design. Biochemical Society Transactions, 31(3), pp. 548-552.

Cerasoli, E., Kelly, S.M., Coggins, J.R., Lapthorn, A.J., Clarke, D.T., and Price, N.C. (2003) Effects of salts on the function and conformational stability of shikimate kinase. Biochimica et Biophysica Acta: Proteins and Proteomics, 1648(1-2), pp. 43-54. (doi:10.1016/S1570-9639(03)00110-9)

Michel, G., Roszak, A.W., Sauve, V., Maclean, J., Matte, A., Coggins, J.R., Cygler, M., and Lapthorn, A.J. (2003) Structures of Shikimate dehydrogenase AroE and its paralog YdiB: a common structural framework for different activities. Journal of Biological Chemistry, 278, pp. 19463-19472. (doi:10.1074/jbc.M300794200)

Dixon, D., McEwen, A., Lapthorn, A., and Edwards, R. (2003) Forced evolution of a herbicide detoxifying glutathione transferase. Journal of Biological Chemistry, 278, pp. 23930-23935. (doi:10.1074/jbc.M303620200)

Kozma, E., Brown, E., Ellis, E.M., and Lapthorn, A.J. (2003) The high resolution crystal structure of rat liver AKR7A1: understanding the substrate specificites of the AKR7 family. Chemico-Biological Interactions, 143, pp. 289-297.

2002

Evans, L. D.B., Roszak, A. W., Noble, L. J., Robinson, D. A., Chalk, P. A., Matthews, J. L., Coggins, J. R., Price, N. C., and Lapthorn, A. J. (2002) Specificity of substrate recognition by type II dehydroquinases as revealed by binding of polyanions. FEBS Letters, 530(1-3), pp. 24-30. (doi:10.1016/S0014-5793(02)03346-X)

Roszak, A.W., Robinson, D.A., Krell, T., Hunter, I.S., Fredrickson, M., Abell, C., Coggins, J.R., and Lapthorn, A.J. (2002) The structure and mechanism of the type II dehydroquinase from Streptomyces coelicolor. Structure, 10(4), pp. 493-503. (doi:10.1016/S0969-2126(02)00747-5)

Kozma, E., Brown, E., Ellis, E., and Lapthorn, A. (2002) The crystal structure of rat liver AKR7A1 - A dimeric member of the aldo-keto reductase superfamily. Journal of Biological Chemistry, 277, pp. 16285-16293. (doi:10.1074/jbc.M110808200)

Thom, R., Cummins, I., Dixon, D., Edwards, R., Cole, D., and Lapthorn, A. (2002) Structure of a tau class glutathione S-transferase from wheat active in herbicide detoxification. Biochemistry, 41, pp. 7008-7020. (doi:10.1021/bi015964x)

2001

Krell, T., Maclean, J., Boam, D.J., Cooper, A., Resmini, M., Brocklehurst, K., Kelly, S.M., Price, N.C., Lapthorn, A.J., and Coggins, J.R. (2001) Biochemical and X-ray crystallographic studies on shikimate kinase: the important structural role of the P-loop lysine. Protein Science, 10(6), pp. 1137-1149. (doi:10.1110/ps.52501)

Thom, R., Dixon, D., Edwards, R., Cole, D., and Lapthorn, A. (2001) The structure of a zeta class glutathione S-transferase from Arabidopsis thaliana: Characterisation of a GST with novel active-site architecture and a putative role in tyrosine catabolism. Journal of Molecular Biology, 308, pp. 949-962. (doi:10.1006/jmbi.2001.4638)

Thom, R., Dixon, D., Edwards, R., Cole, D., and Lapthorn, A. (2001) Structure determination of zeta class glutathione transferase from Arabidopsis thaliana. Chemico-Biological Interactions, 133, pp. 53-54.

This list was generated on Tue Mar 31 06:24:23 2015 BST.
Jump to: Articles
Number of items: 30.

Articles

Turton, D. A., Senn, H., Harwood, T., Lapthorn, A. J., Ellis, E. M., and Wynne, K. (2014) Terahertz underdamped vibrational motion governs protein-ligand binding in solution. Nature Communications, 5, p. 3999. (doi:10.1038/ncomms4999)

Turton, D., Harwood, T., Lapthorn, A., Ellis, E., and Wynne, K. (2013) Ultrabroadband terahertz spectroscopies of biomolecules and water. Proceedings of SPIE, 8623(862303), (doi:10.1117/12.2003796)

Paz, S. et al. (2011) Tetrahydrobenzothiophene derivatives: conformationally restricted inhibitors of type II dehydroquinase. ChemMedChem, 6(2), pp. 266-272. (doi:10.1002/cmdc.201000343)

Tran, A.T., Cergol, K.M. , West, N.P. , Randall, E.J. , Britton, W.J. , Bokhari, S.A.I. , Ibrahim, M. , Lapthorn, A.J., and Payne, R.J. (2011) Synthesis and evaluation of potent ene-yne inhibitors of type II dehydroquinases as tuberculosis drug leads. ChemMedChem, 6(2), pp. 262-265. (doi:10.1002/cmdc.201000399)

Hendry, E., Carpy, T., Johnston, J., Popland, M., Mikhaylovskiy, R.V., Lapthorn, A., Kelly, S.M., Barron, L.D., Gadegaard, N., and Kadodwala, M. (2010) Ultrasensitive detection and characterization of biomolecules using superchiral fields. Nature Nanotechnology, 5(11), pp. 783-787. (doi:10.1038/nnano.2010.209 )

Tran, A.T., Cergol, K.M., Britton, W.J., Bokhari, S.A.I., Ibrahim, M., Lapthorn, A.J., and Payne, R.J. (2010) Rapid assembly of potent type II dehydroquinase inhibitors via "Click" chemistry. MedChemComm, 1(4), pp. 271-275. (doi:10.1039/c0md00097c )

Dixon, D. P., Lapthorn, A., Madesis, P., Mudd, E. A., Day, A., and Edwards, R. (2008) Binding and glutathione conjugation of porphyrinogens by plant glutathione transferases. Journal of Biological Chemistry, 283(29), pp. 20268-20276. (doi:10.1074/jbc.M802026200 )

Stewart, K. A., Robinson, D. A., and Lapthorn, A. J. (2008) Type II dehydroquinase: molecular replacement with many copies. Acta Crystallographica. Section D: Biological Crystallography, 64, pp. 108-118. (doi:10.1107/S0907444907054923 )

Payne, R.J., Riboldi-Tunnicliffe, A., Kerbarh, O., Abell, A.D., Lapthorn, A.J., and Abell, C. (2007) Design, synthesis, and structural studies on potent biaryl inhibitors of type II dehydroquinases. ChemMedChem, 2(7), pp. 1010-1013. (doi:10.1002/cmdc.200700062)

Lohkamp, B., Paterson, N., and Lapthorn, A. (2006) Application of a new vector system for efficient protein purification in the crystallization of PA5104/ORF from Pseudomonas aeruginosa. Journal of Structural Biology, 155, pp. 111-113. (doi:10.1016/j.jsb.2006.03.016)

Robinson, D.A.,, Stewart, K.A., Price, N.C., Chalk, P.A., Coggins, J.R., and Lapthorn, A.J. (2006) Crystal structures of Helicobacter pylori type II dehydroquinase inhibitor complexes: new directions for inhibitor design. Journal of Medicinal Chemistry, 49(4), pp. 1282-1290. (doi:10.1021/jm0505361)

Zhu, X., Lapthorn, A., and Ellis, E. (2006) Crystal structure of mouse succinic semialdehyde reductase AKR7A5: Structural basis for substrate specificity. Biochemistry, 45, pp. 1562-1570. (doi:10.1021/bi051610k)

Dias-Gunasekara, S., Gubbens, J., van Lith, M., Dunne, C., Williams, J.A.G., Kataky, R., Scoones, D., Lapthorn, A., Bulleid, N.J., and Benham, A.M. (2005) Tissue-specific expression and dimerization of the endoplasmic reticulum oxidoreductase Ero1β. Journal of Biological Chemistry, 280, pp. 33066-33075. (doi:10.1074/jbc.M505023200)

Charrel-Dennis, M. et al. (2005) The human chorionic gonadotropin-beta arginine(68) to glutamic acid substitution fixes the conformation of the C-terminal peptide. Molecular Endocrinology, 19, pp. 1803-1811. (doi:10.1210/me.2004-0109)

Dixon, D., Lapthorn, A., and Edwards, R. (2005) Synthesis and analysis of chimeric maize glutathione transferases. Plant Science, 168, pp. 873-881. (doi:10.1016/j.plantsci.2004.11.009)

Toscano, M.D., Stewart, K.A., Coggins, J.R., Lapthorn, A.J., and Abell, C. (2005) Rational design of new bifunctional inhibitors of type II dehydroquinase. Organic and Biomolecular Chemistry, 3, pp. 3102-3104. (doi:10.1039/B507156A)

Lohkamp, B., McDermott, G., Campbell, S.A., Coggins, J.R., and Lapthorn, A.J. (2004) The structure of Escherichia coli ATP-phosphoribosyltransferase: identification of substrate binding sites and mode of AMP inhibition. Journal of Molecular Biology, 336(1), pp. 131-144. (doi:10.1016/j.jmb.2003.12.020)

Charrel-Dennis, M., Jackson, A., Lund, T., Lapthorn, A., Berger, P., Roitt, I., and Delves, P. (2004) The major hormone-specific discontinuous epitopes on human chorionic gonadotrophin. Journal of Molecular Endocrinology, 32, pp. 571-581.

Coggins, J.R., Abell, C., Evan, L.B., Frederickson, M., Robinson, D.A., Roszak, A.W., and Lapthorn, A.P. (2003) Experiences with the Shikimate-pathway enzymes as targets for rational drug design. Biochemical Society Transactions, 31(3), pp. 548-552.

Cerasoli, E., Kelly, S.M., Coggins, J.R., Lapthorn, A.J., Clarke, D.T., and Price, N.C. (2003) Effects of salts on the function and conformational stability of shikimate kinase. Biochimica et Biophysica Acta: Proteins and Proteomics, 1648(1-2), pp. 43-54. (doi:10.1016/S1570-9639(03)00110-9)

Michel, G., Roszak, A.W., Sauve, V., Maclean, J., Matte, A., Coggins, J.R., Cygler, M., and Lapthorn, A.J. (2003) Structures of Shikimate dehydrogenase AroE and its paralog YdiB: a common structural framework for different activities. Journal of Biological Chemistry, 278, pp. 19463-19472. (doi:10.1074/jbc.M300794200)

Dixon, D., McEwen, A., Lapthorn, A., and Edwards, R. (2003) Forced evolution of a herbicide detoxifying glutathione transferase. Journal of Biological Chemistry, 278, pp. 23930-23935. (doi:10.1074/jbc.M303620200)

Kozma, E., Brown, E., Ellis, E.M., and Lapthorn, A.J. (2003) The high resolution crystal structure of rat liver AKR7A1: understanding the substrate specificites of the AKR7 family. Chemico-Biological Interactions, 143, pp. 289-297.

Evans, L. D.B., Roszak, A. W., Noble, L. J., Robinson, D. A., Chalk, P. A., Matthews, J. L., Coggins, J. R., Price, N. C., and Lapthorn, A. J. (2002) Specificity of substrate recognition by type II dehydroquinases as revealed by binding of polyanions. FEBS Letters, 530(1-3), pp. 24-30. (doi:10.1016/S0014-5793(02)03346-X)

Roszak, A.W., Robinson, D.A., Krell, T., Hunter, I.S., Fredrickson, M., Abell, C., Coggins, J.R., and Lapthorn, A.J. (2002) The structure and mechanism of the type II dehydroquinase from Streptomyces coelicolor. Structure, 10(4), pp. 493-503. (doi:10.1016/S0969-2126(02)00747-5)

Kozma, E., Brown, E., Ellis, E., and Lapthorn, A. (2002) The crystal structure of rat liver AKR7A1 - A dimeric member of the aldo-keto reductase superfamily. Journal of Biological Chemistry, 277, pp. 16285-16293. (doi:10.1074/jbc.M110808200)

Thom, R., Cummins, I., Dixon, D., Edwards, R., Cole, D., and Lapthorn, A. (2002) Structure of a tau class glutathione S-transferase from wheat active in herbicide detoxification. Biochemistry, 41, pp. 7008-7020. (doi:10.1021/bi015964x)

Krell, T., Maclean, J., Boam, D.J., Cooper, A., Resmini, M., Brocklehurst, K., Kelly, S.M., Price, N.C., Lapthorn, A.J., and Coggins, J.R. (2001) Biochemical and X-ray crystallographic studies on shikimate kinase: the important structural role of the P-loop lysine. Protein Science, 10(6), pp. 1137-1149. (doi:10.1110/ps.52501)

Thom, R., Dixon, D., Edwards, R., Cole, D., and Lapthorn, A. (2001) The structure of a zeta class glutathione S-transferase from Arabidopsis thaliana: Characterisation of a GST with novel active-site architecture and a putative role in tyrosine catabolism. Journal of Molecular Biology, 308, pp. 949-962. (doi:10.1006/jmbi.2001.4638)

Thom, R., Dixon, D., Edwards, R., Cole, D., and Lapthorn, A. (2001) Structure determination of zeta class glutathione transferase from Arabidopsis thaliana. Chemico-Biological Interactions, 133, pp. 53-54.

This list was generated on Tue Mar 31 06:24:23 2015 BST.