Institute of Neuroscience and Psychology

Prof I Mhairi Macrae

  • Professor of Neuroscience (Stroke and Brain Imaging)

telephone: 01413306978
email: Mhairi.Macrae@glasgow.ac.uk


Mhairi Macrae is a neuroscientist with an undergraduate and post-graduate degree in Pharmacology from the University of Glasgow.

Her main research interest is in stroke and cerebral blood flow (CBF), studying ischaemic brain injury using  in vivo rodent models, imaging techniques including MRI and behavioural testing. 

She has expertise in the development of animal models of experimental stroke, in the measurement of CBF and ischaemic damage and in the assessment of neurological deficits and functional recovery. Her research has included studies of CBF, brain metabolism, cerebral autoregulation, oxidative stress, inflammation and stroke sensitivity genes.

She is Deputy Head of Stroke and Brain Imaging, with her group based in the neuroscience laboratories of the Wellcome Surgical Institute (WSI),  Garscube Estate.

 

She is also Co-Director of the University of Glasgow Glasgow Experimental MRI Centre, situated adjacent to WSI in Garscube Estate.

Research

Current research interests related to stroke include the ischaemic penumbra, inflammation, hyperglycaemia and metabolic syndrome,  stem cell therapy and the influence of ageing.

She has a particular interest in neuroprotection strategies in focal cerebral ischaemia which has included studies on glutamate antagonists, anti-oxidants and oestrogen.  Her research also  involves identification of mechanisms responsible for inducing brain damage during ischaemia and reperfusion, and those promoting brain repair and  functional recovery.

A more recent research focus has been the use of MRI in neuroscience research with a particular interest in brain imaging.

Cerebral Blood Flow and the Ischaemic Penumbra

Aims & techniques: Investigation of the ischaemic penumbra and characterisation of changes in CBF is carried out in a range of experimental stroke models using blood flow autoradiography, laser Doppler flowmetry and, more recently, MRI techniques.

Collaborators: Debbie Dewar, Chris McCabe and William Holmes and Keith Muir (Centre for Stroke and Brain Imaging), Barrie Condon and David Brennan (Clinical Physics), Celestine Santosh and Donald Hadley (Neuroradiology), Lorraine Work (Institute of Cardiovascular and Medical Sciences)

Oxidative Stress and Inflammation

Aims & techniques: The role of oxidative stress and inflammation in stroke is studied in normotensive and hypertensive (SHRSP) rat strains using immunohistochemistry and histology techniques to identify inflammatory cells and oxidative damage.

Collaborators: Anna Dominiczak and her group (Division of Cardiovascular and Medical Sciences), Hilary Carswell (Strathclyde Institute of Pharmacy & Biomedical Sciences, University of Strathclyde) and David I Graham (Division of Clinical Neuroscience).

 Oestrogen and Stroke

Aims & techniques: Brain aromatase and the influence of oestrogen and  oestrogenic drugs on stroke outcome are studied using blood flow techniques, histology & MRI assessment of infarct volume, immunohistochemistry and behavioural testing of sensorimotor function. 

Collaborators: Hilary Carswell (Strathclyde Institute of Pharmacy & Biomedical Sciences University of Strathclyde), Karen Horsburgh (Neuroscience, University of Edinburgh), Anna Dominiczak and her group (Division of Cardiovascular and Medical Sciences).

Experimental stroke studies in the spontaneously hypertensive stroke-prone  (SHRSP) rat

Techniques: Stroke sensitivity and recovery from stroke in the SHRSP are studied using blood flow techniques, MRI assessment of penumbra,  histology & MRI assessment of infarct volume, immunohistochemistry,  and behavioural testing of sensorimotor function.

Collaborators: Lorraine Work and Delyth Graham  (Institute of Cardiovascular and Medical Sciences), Debbie Dewar and Chris McCabe (Centre for Stroke and Brain Imaging).

Protein GADD34 and its role in Stroke

Aims & techniques: The role of protein GADD34 in brain ischaemia has been studied in rodents, including transgenic GADD34 knock-outs and in human post-mortem brain from stroke and cardiac arrest cases.  Histology & MRI are used for quantitation of infarct volume, immunohistochemistry to identify changes in GADD34 protein expression  and autoradiography to quantify protein synthesis inhibition in ischaemic brain.

Collaborators: Chris McCabe, David Graham (Division of Clinical Neuroscience), Hide Imai (Gunma University, Japan), S Moira Brown and June Harland (Crusade Laboratories).

White matter damage in Experimental Stroke

Aims & techniques: Rodent models of lacunar stroke and methodology to quantify white matter damage  have been developed to study the effects of ischaemia on white matter and the influence of drugs on white matter damage

Collaborators: Hide Imai (Gunma University, Japan), Keith Muir (Division of Clinical Neuroscience) Ken Lees (Division of Cardiovascular and Medical Sciences), Chris Weir (Roberson Centre for Biostatistics), Clotilde Lecrux and Omar Touzani (University of Caen, France)

Jump to: 2012 | 2011 | 2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002 | 2001
Number of items: 42.

2012

Baskerville, T., McCabe, C. , Macrae, I.M. , and Holmes, W. (2012) Noninvasive MRI measurement of CBF: evaluating an arterial spin labelling sequence with 99mTc- HMPAO CBF autoradiography in a rat stroke model. Journal of Cerebral Blood Flow & Metabolism . ISSN 0271-678X (doi:10.1038/jcbfm.2012.19)

Bingham, D., Martin, S.J., Macrae, I.M. , and Carswell, H.V.O. (2012) Watermaze performance after middle cerebral artery occlusion in the rat: the role of sensorimotor versus memory impairments. Journal of Cerebral Blood Flow & Metabolism . ISSN 0271-678X (In Press)

Holmes, W.M., Lopez-Gonzalez, M.R., Gallagher, L. , Deuchar, G. , Macrae, I. , and Santosh, C. (2012) Novel MRI detection of the ischemic penumbra: direct assessment of metabolic integrity. Nmr in Biomedicine, 25 (2). pp. 295-304. ISSN 0952-3480 (doi:10.1002/nbm.1748)

Reid, E., Graham, D., Lopez Gonzalez, M.R. , Holmes, W.M. , Macrae, I.M. , and McCabe, C. (2012) Penumbra detection using PWI/DWI mismatch MRI in a rat stroke model with and without co-morbidity: comparison of methods. Journal of Cerebral Blood Flow & Metabolism . ISSN 0271-678X (In Press)

Wetterling, F., Gallagher, L. , Macrae, I.M. , Junge, S., and Fagan, A.J. (2012) Regional and temporal variations in tissue sodium concentration during the acute stroke phase. Magnetic Resonance in Medicine, 67 (3). pp. 740-749. ISSN 0740-3194 (doi:10.1002/mrm.23031)

Wetterling, F., Hogler, M., Molkenthin, U., Junge, S., Gallagher, L., Macrae, I.M. , and Fagan, A.J. (2012) The design of a double-tuned two-port surface resonator and its application to in vivo Hydrogen- and Sodium-MRI. Journal of Magnetic Resonance . ISSN 1090-7807 (In Press)

2011

Macrae, I.M. (2011) Preclinical stroke research - advantages and disadvantages of the most common rodent models of focal ischaemia. British Journal of Pharmacology, 164 (4). pp. 1062-1078. ISSN 0007-1188 (doi:10.1111/j.1476-5381.2011.01398.x)

Baskerville, T., Deuchar, G.A. , McCabe, C. , Robertson, C.A., Holmes, W.M. , Santosh, C. , and Macrae, I.M. (2011) Influence of 100% and 40% oxygen on penumbral blood flow, oxygen level, and T2*-weighted MRI in a rat stroke model. Journal of Cerebral Blood Flow and Metabolism, 31 (8). pp. 1799-1806. ISSN 0271-678X (doi:10.1038/jcbfm.2011.65)

Robertson, C.A., McCabe, C. , Gallagher, L. , Lopez Gonzalez, M. del , Holmes, W.M. , Condon, B. , Muir, K.W. , Santosh, C. , and Macrae, I.M. (2011) Stroke penumbra defined by an MRI-based oxygen challenge technique: 1. validation using [14C]2-deoxyglucose autoradiography. Journal of Cerebral Blood Flow & Metabolism, 31 (8). pp. 1778-1787. ISSN 0271-678X (doi:10.1038/jcbfm.2011.66)

Robertson, C.A., McCabe, C. , Gallagher, L. , Lopez Gonzalez, M. del , Holmes, W.M. , Condon, B. , Muir, K.W. , Santosh, C. , and Macrae, I.M. (2011) Stroke penumbra defined by an MRI-based oxygen challenge technique: 2. Validation based on the consequences of reperfusion. Journal of Cerebral Blood Flow & Metabolism, 31 (8). pp. 1788-1798. ISSN 0271-678X (doi:10.1038/jcbfm.2011.67)

2010

Carswell, H.V.O., Macrae, I. M. , and Farr, T.D. (2010) Complexities of oestrogen in stroke. Clinical Science, 118 (5-6). pp. 375-389. ISSN 0143-5221 (doi:10.1042/CS20090018)

Dani, K.A., Santosh, C. , Brennan, D. , McCabe, C. , Holmes, W.M. , Condon, B. , Hadley, D.M. , Macrae, I.M. , Shaw, M. , and Muir, K.W. (2010) T2*-weighted magnetic resonance imaging with hyperoxia in acute ischemic stroke. Annals of Neurology, 68 (1). pp. 37-47. ISSN 0364-5134 (doi:10.1002/ana.22032)

McCabe, C., Gallagher, L. , Macrae, I.M. , Gsell, W., Graham, D. , and Dominiczak, A.F. (2010) Response to letter by Yao (Differences in the evolution of the ischemic penumbra in stroke-prone spontaneously hypertensive and Wistar-Kyoto rats). Stroke, 41 (4). E198-E199. ISSN 0039-2499 (doi:10.1161/STROKEAHA.109.575704)

Wetterling, F., Tabbert, M., Junge, S., Gallagher, L. , Macrae, I.M. , and Fagan, A.J. (2010) A double-tuned1H/23Na dual resonator system for tissue sodium concentration measurements in the rat brain via Na-MRI. Physics in Medicine and Biology, 55 (24). pp. 7681-7695. ISSN 0031-9155 (doi:10.1088/0031-9155/55/24/019)

2009

McCabe, C., Gallagher, L. , Gsell, W., Graham, D. , Dominiczak, A.F. , and Macrae, I.M. (2009) Differences in the evolution of the ischemic penumbra in stroke-prone spontaneously hypertensive and Wistar-Kyoto rats. Stroke, 40 (12). pp. 3864-3868. ISSN 0039-2499 (doi:10.1161/STROKEAHA.109.559021)

Shah, IM, Macrae, IM , and Di Napoli, M (2009) Neuroinflammation and Neuroprotective Strategies in Acute Ischaemic Stroke - from bench to bedside. Current Molecular Medicine, 9 (3). pp. 336-354.

2008

Santosh, C. et al. (2008) Potential use of oxygen as a metabolic biosensor in combination with T2*-weighted MRI to define the ischemic penumbra. Journal of Cerebral Blood Flow and Metabolism, 28 (10). pp. 1742-1753. ISSN 0271-678X (doi:10.1038/jcbfm.2008.56)

McCabe, C., White, F., Brown, S.M., and Macrae, I.M. (2008) GADD34 gene restores virulence in viral vector used in experimental stroke study. Journal of Cerebral Blood Flow & Metabolism, 28 (4). pp. 747-751. ISSN 0271-678X (doi:10.1038/sj.jcbfm.9600565)

Lecrux, C., McCabe, C. , Weir, C.J., Gallagher, L. , Mullin, J. , Touzani, O., Muir, K. , Lees, K.R. , and Macrae, I.M. (2008) Effects of magnesium treatment in a model of internal capsule lesion in spontaneously hypertensive rats. Stroke, 39 (2). pp. 448-454. ISSN 0039-2499 (doi:10.1161/STROKEAHA.107.492934)

Fagan, AJ, Mullin, JM, Gallagher, L , Hadley, DM , Macrae, IM , and Condon, B (2008) Serial postmortem relaxometry in the normal rat brain and following stroke. Journal of Magnetic Resonance Imaging, 27 (3). pp. 469-475.

Farr, TD, Carswell, HVO, Mccann, DJ, Sato, M, Bryant, HU, Dodge, JA, and Macrae, IM (2008) The selective oestrogen receptor modulator, LY362321, is not neuroprotective in a rat model of transient focal ischaemia. Journal of Neuroendocrinology, 20 (3). pp. 366-374.

2007

Farr, TD, Carswell, HVO, Gsell, W, and Macrae, IM (2007) Estrogen receptor beta agonist diarylpropiolnitrile (DPN) does not mediate neuroprotection in a rat model of permanent focal ischemia. Brain Research, 1185 . pp. 275-282.

2006

Morton, E., Macrae, I.M. , McCabe, C. , Brown, S.M., and White, F. (2006) Identification of the growth arrest and DNA damage protein GADD34 in the normal human heart and demonstration of alterations in expression following myocardial ischaemia. International Journal of Cardiology, 107 (1). pp. 126-129. ISSN 0167-5273 (doi:10.1016/j.ijcard.2005.01.051)

Farr, TD, Carswell, HVO , Gallagher, L, Condon, B, Fagan, AJ, Mullin, J, and Macrae, IM (2006) 17 beta-estradiol treatment following permanent focal ischemia does not influence recovery of sensorimotor function. Neurobiology of Disease, 23 . pp. 552-562. (doi:10.1016/j.nbd.2006.04.009)

Macrae, IM, and Carswell, HV (2006) Oestrogen and stroke: the potential for harm as well as benefit. Biochemical Society Transactions, 34 . pp. 1362-1365.

2005

Bingham, D, Macrae, IM , and Carswell, HV (2005) Detrimental effects of 17 beta-oestradiol after permanent middle cerebral artery occlusion. Journal of Cerebral Blood Flow and Metabolism, 25 . pp. 414-420. (doi:10.1038/sj.jcbfm.9600031)

Brown, SM, Gallagher, L, Graham, DI, Harland, J, Imai, H, Macrae, IM , and McCaig, D (2005) Evolution of GADD34 expression after focal cerebral ischaemia. Brain Research, 1034 . pp. 51-61. (doi:10.1016/j.brainres.2004.11.058)

Carswell, HV, Dominiczak, AF , Garcia-Segura, LM, Harada, N, Hutchison, JB, and Macrae, IM (2005) Brain aromatase expression after experimental stroke: Topography and time course. Journal of Steroid Biochemistry and Molecular Biology, 96 . pp. 89-91. (doi:10.1016/j.jsbmb.2005.02.016)

Gordon, KB, Macrae, IM , and Carswell, HVO (2005) Effects of 17 beta-oestradiol on cerebral ischaemic damage and lipid peroxidation. Brain Research, 1036 . pp. 155-162. (doi:10.1016/j.brainres.2004.12.052)

McGill, J.K., Gallagher, L., Carswell, H.V.O., Irving, E.A., Dominiczak, A.F. , and Macrae, I.M. (2005) Impaired functional recovery after stroke in the stroke-prone spontaneously hypertensive rat. Stroke, 36 (1). pp. 135-141. ISSN 0039-2499 (doi:10.1161/01.STR.0000149629.32525.b7)

Nemoto, K. et al. (2005) No involvement of the nerve growth factor gene locus in hypertension in spontaneously hypertensive rats. Hypertension Research, 28 (2). pp. 155-163. ISSN 0916-9636

2004

Antier, D, Carswell, HV, Brosnan, MJ, Hamilton, CA , Macrae, IM , Groves, S, Jardine, E, Reid, JL , and Dominiczak, AF (2004) Increased levels of superoxide in brains from old female rats. Free Radical Research, 38 . pp. 177-183. (doi:10.1080/10715760310001643294)

Brown, SM, Graham, DI, Harland, J, Macrae, IM , McCaig, D, and White, F (2004) Up-regulation of a growth arrest and DNA damage protein (GADD34) in the ischaemic human brain: implications for protein synthesis regulation and DNA repair. Neuropathology and Applied Neurobiology, 30 . pp. 683-691. (doi:10.1111/j.1365-2990.2004.00584.x)

Carswell, HV, Bingham, D, Wallace, K, Nilsen, M, Graham, DI, Dominiczak, AF , and Macrae, IM (2004) Differential effects of 17 beta-estradiol upon stroke damage in stroke prone and normotensive rats. Journal of Cerebral Blood Flow and Metabolism, 24 . pp. 298-304. (doi:10.1097/01.WCB.0000112322.75217.FD)

Carswell, HVO, Gallagher, L, Harrop, E, Horsburgh, KJ, and Macrae, IM (2004) Neuroprotection by a selective estrogen receptor beta agonist in a mouse model of global ischemia. American Journal of Physiology-heart and Circulatory Physiology, 287 . H1501-H1504. (doi:10.1152/ajpheart.00227.2004)

2003

Graham, DI, Imai, H, Macrae, IM , and Masayasu, H (2003) Antioxidant ebselen reduces oxidative damage in focal cerebral ischemia. Free Radical Biology and Medicine, 34 . pp. 56-63.

2002

Brown, SM, Graham, DI, Harland, J, Imai, H, Macrae, IM , and McCulloch, J (2002) Specific expression of the cell cycle regulation proteins, GADD34 and PCNA, in the peri-infarct zone after focal cerebral ischaemia in the rat. European Journal of Neuroscience, 15 . pp. 1929-1936. (doi:10.1046/j.1460-9568.2002.02025.x)

Graham, DI, Imai, H, Macrae, IM , Masayasu, H, and McCulloch, J (2002) New method for the quantitative assessment of axonal damage in focal cerebral ischemia. Journal of Cerebral Blood Flow and Metabolism, 22 . pp. 1080-1089.

Horsburgh, K, Macrae, IM , and Carswell, H (2002) Estrogen is neuroprotective via an apolipoprotein E-dependent mechanism in a mouse model of global ischemia. Journal of Cerebral Blood Flow and Metabolism, 22 . pp. 1189-1195.

McBride, MW, Carswell, HV , Graham, D, Carr, FJ, Charchar, FJ, Macrae, IM , and Dominiczak, AF (2002) Genetic and gender determinants of cerebrovascular disease. Seminars in Nephrology, 22 . pp. 127-134. (doi:10.1053/snep.2002.30213)

2001

Marks, L, Carswell, HVO , Peters, EE, Graham, DI, Patterson, J, Dominiczak, AF , and Macrae, IM (2001) Characterization of the microglial response to cerebral ischemia in the stroke-prone spontaneously hypertensive rat. Hypertension, 38 . pp. 116-122.

Negrin, CD, McBride, MW , Carswell, HVO , Graham, D, Carr, FJ, Clark, JS , Jeffs, B, Anderson, NH, Macrae, IM , and Dominiczak, AF (2001) Reciprocal consomic strains to evaluate Y chromosome effects. Hypertension, 37 . pp. 391-397.

This list was generated on Thu May 24 14:50:58 2012 BST.
  • Supporting the ischaemic brain with oxygen-carrying perfluorocarbons.
    Scottish Executive Health Department
    2012 - 2013
     
  • Metabolic MRI in Acute Stroke; Development and Validation of the 'Oxygen Challenge' Technique
    Scottish Executive Health Department
    2011 - 2013
     
  • Staff Salary
    NHS Greater Glasgow & Clyde
    2011 - 2011
     
  • Sodium imaging following an "Oxygen Challenge" to assess function of the N/K ATP pump following ischaemia to demonstrate membrane integrity: A novel technique to identify the functional "Penumbra"?
    Neurosciences Foundation
    2011 - 2012
     
  • Sodium imaging following an "Oxygen Challenge" to assess function of the N/K ATP pump following ischaemia to demonstrate membrane integrity: A novel technique to identify the functional "Penumbra"?
    Neurosciences Foundation
    2011 - 2012
     
  • Development and application of oscillatory gradient spin echo in stroke animal model at 7T and further validation with histology
    Neurosciences Foundation
    2011 - 2012
     
  • SINAPSE-SPIRIT: Novel and collaborative approaches to knowledge exchange in translational imaging
    Scottish Funding Council
    2010 - 2013
     
  • Metabolic syndrome, hyperglycemia and experimental stroke
    Scottish Executive Health Department
    2009 - 2010
     
  • Development and Validation of an MRI Method to Image Cerebral Metabolism using Oxygen 17.
    Neurosciences Foundation
    2009 - 2009
     
  • Assessment of Myoglobin as a Novel MR Reporter Gene and its potential to protect Embryonic Stem Cells against hypoxia
    Neurosciences Foundation
    2009 - 2010
     
  • A combined approach targeting oxidative stress and apoptosis in stroke
    British Heart Foundation
    2008 - 2012
     
  • Developing a novel diagnostic imaging product for assessing metabolism and its application in stroke
    Scottish Enterprise
    2008 - 2010
     
  • Imaging the ischaemic penumbra using BOLD MRI with oxygen challenge as a biotracer
    MRC
    2008 - 2011
     
  • Stem cell research in experimental stroke
    British Council, India
    2008 - 2009
     
  • Brain'07 & BrainPET'07 the 23rd international symposium on cerebral blood flow, metabolism and quantification of brain function with PET
    Royal Society
    2007 - 2007
     
  • Characterising the ischaemic penumbra in the SIIRSP and WKY following focal cerebral ischaemia
    Neurosciences Foundation
    2006 - 2007
     
  • Neuroscience 2006
    Royal Society
    2006 - 2006
     
  • Targeted gene therapy for cardiovascular disease
    MRC
    2005 - 2008
     
  • Brain'05 & BrainPET'05 XXIInd International Symposium on Cerebral Blood Flow, Metabolism and Function
    Royal Society
    2005 - 2005
     
  • Can BOLD MRI imaging with an 02 challenge delineate the ischaemic penumbra in stroke?
    Neurosciences Foundation
    2005 - 2006
     
  • The role of GADD34 in cerebral ischaemia - an investigation of focal ischaemia in the GADD 34 knockout mouse
    Neurosciences Foundation
    2005 - 2007
     
  • Non-Invasive Imaging In Neuroscience: Small Bore High Field Magnetic Resonance Facility
    Scottish Higher Education Funding Council
    2002 - 2007