Institute of Infection, Immunity and Inflammation

Prof James Brewer

  • Chair in Basic Immunology (Infection Immunity and Inflammation Medicine)

telephone: 01413308417
email: James.Brewer@glasgow.ac.uk


Current Research :

Imaging Host & Parasite responses in vivo.

Much of what we understand about the anatomy and architecture of the immune system was revealed through exquisite experiments performed in the 1950’s to 70’s. These studies identified the role that anatomy played in a number of fundamental immunological phenomena including recirculation, induction of immune priming or tolerance and the interactions of T and B cells. The recent resurgence of interest in the role of immune architecture and anatomy in basic immunological and infectious phenomena is almost entirely due to technological developments in identifying and tracking cells and parasites in vivo, not least through the ability to do this dynamically, in real time through the application of multiphoton microscopy. We have recently established a state of the art, multiphoton microscope system at the University of Glasgow, which allows us to image on more channels, faster and deeper than previously possible. Our current studies apply multiphoton and other in vivo approaches to analyse immune priming and tolerance including studies in the context of Plasmodium and Trypanosome infection. This work provides a visual insight into host/parasite interactions and highlight the importance of early cellular dialogue in the development of adaptive immune responses. We also collaborate with physicists, mathematicians and chemists to continue development of new non-invasive, translational cell-imaging approaches.

Jump to: 2012 | 2011 | 2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002 | 2001
Number of items: 49.

2012

Afolaranmi, G.A., Akbar, M. , Brewer, J. , and Grant, M.H. (2012) Distribution of metal released from cobalt-chromium alloy orthopaedic wear particles implanted into air pouches in mice. Journal of Biomedical Materials Research Part A, 100A (6). pp. 1529-1538. ISSN 1549-3296 (doi:10.1002/jbm.a.34091)

Gibson, V.B. et al. (2012) A novel method to allow noninvasive, longitudinal imaging of the murine immune system in vivo. Blood, 119 (11). pp. 2545-2551. ISSN 0006-4971 (doi:10.1182/blood-2011-09-378356)

Hutchison, S., Benson, R.A. , Gibson, V.B. , Pollock, A.H., Garside, P. , and Brewer, J.M. (2012) Antigen depot is not required for alum adjuvanticity. FASEB Journal, 26 (3). pp. 1272-1279. ISSN 0892-6638 (doi:10.1096/fj.11-184556)

2011

Butcher, J.P., Malcolm, J., Benson, R.A. , Deng, D.M., Brewer, J.M. , Garside, P. , and Culshaw, S. (2011) Effects of streptococcus mutans on dendritic cell activation and function. Journal of Dental Research, 90 (10). pp. 1221-1227. ISSN 0022-0345 (doi:10.1177/0022034511412970)

Conigliaro, P., Benson, R.A. , Patakas, A. , Kelly, S.M., Valesini, G., Holmdahl, R., Brewer, J.M. , McInnes, I.B. , and Garside, P. (2011) Characterization of the anticollagen antibody response in a new model of chronic polyarthritis. Arthritis and Rheumatism, 63 (8). pp. 2299-2308. ISSN 0004-3591 (doi:10.1002/art.30413)

Akbar, M., Brewer, J.M. , and Grant, M.H. (2011) Effect of chromium and cobalt ions on primary human lymphocytesin vitro. Journal of Immunotoxicology, 8 (2). pp. 140-149. ISSN 1547-691X (doi:10.3109/1547691X.2011.553845)

Benson, R.A., Patakas, A. , McQueenie, R., Ross, K. , McInnes, I.B. , Brewer, J.M. , and Garside, P. (2011) Arthritis in space and time – to boldly go! Febs Letters, 585 (23). pp. 2640-3648. ISSN 0014-5793 (doi:10.1016/j.febslet.2011.04.069)

Conigliaro, P., Benson, R.A. , Patakas, A., Kelly, S.M. , Valesini, G., Homdahl, R., Brewer, J.M. , McInnes, I.B. , and Garside, P. (2011) Characterization of the anti-collagen antibody response in a new model of chronic polyarthritis. Arthritis and Rheumatism, 63 (8). pp. 2299-2308. ISSN 0004-3591 (doi:10.1002/art.30413)

Fumagalli, S. et al. (2011) In Vivo Real-Time Multiphoton Imaging of T Lymphocytes in the Mouse Brain After Experimental Stroke. Stroke, 42 (5). pp. 1429-1436. ISSN 0039-2499 (doi:10.1161/STROKEAHA.110.603704)

Patakas, A., Platt, A.M. , Butcher, J.P., Maffia, P. , McInnes, I.B. , Brewer, J.M. , Garside, P. , and Benson, R.A. (2011) Putative existence of reciprocal dialogue between Tfh and B cells and its impact on infectious and autoimmune disease. Immunology Letters, 138 (1). pp. 38-46. ISSN 0165-2478 (doi:10.1016/j.imlet.2011.03.002)

2010

Platt, A.M., Gibson, V.B. , Patakas, A., Benson, R.A. , Nadler, S.G., Brewer, J.M. , McInnes, I. , and Garside, P. (2010) Abatacept limits breach of self-tolerance in a Murine model of arthritis via effects on the generation of T follicular helper cells. Journal of Immunology, 185 (3). pp. 1558-1567. ISSN 0022-1767 (doi:10.4049/jimmunol.1001311)

Benson, R.A., Patakas, A., Conigliaro, P. , Rush, C. M. , Garside, P. , McInnes, I. B. , and Brewer, J. M. (2010) Identifying the Cells Breaching Self-Tolerance in Autoimmunity. Journal of Immunology, 184 (11). pp. 6378-6385. ISSN 0022-1767 (doi:10.4049/jimmunol.0903951)

Ortolano, F., Maffia, P. , Dever, G., Millington, O. , De Simoni, M., Brewer, J. , Bushell, T.J., Garside, P. , and Carswell, H.V. (2010) Advances in imaging of new targets for pharmacological intervention in stroke: real-time tracking of T-cells in the ischaemic brain. British Journal of Pharmacology, 159 (4). pp. 808-811. ISSN 0007-1188 (doi:10.1111/j.1476-5381.2009.00527.x)

Conigliaro, P., Perricone, C., Benson, R.A. , Garside, P. , Brewer, J.M. , Perricone, R., and Valesini, G. (2010) The type I IFN system in rheumatoid arthritis. Autoimmunity, 43 (3). pp. 220-225. ISSN 0891-6934 (doi:10.3109/08916930903510914)

Garside, P., and Brewer, J. (2010) In vivo imaging of infection immunology—4I’s! Seminars in Immunopathology, 32 (3). pp. 289-296. ISSN 1863-2297 (doi:10.1007/s00281-010-0215-y)

Grassia, G., Dever, G. , Gordon, P., McInnes, I. , Spanbroek, R., Habenicht, A.J.R., Brewer, J. , Garside, P. , and Maffia, P. (2010) Real time imaging of lymphocytes in APOE-/- mouse aortic tertiary lymphoid organs. In: Spring Meeting of the British-Society-for-Cardiovascular-Research 2010, 07-08 June 2010, Manchester, UK.

Millington, O., Brewer, J. , Garside, P. , and Maffia, P. (2010) Imaging interactions between the immune and cardiovascular systems in vivo by multiphoton microscopy. In: Marelli-Berg, F.M. and Nourshargh, S. (eds.) Methods in Molecular Biology. Series: T-Cell Trafficking: Methods and Protocols, 616 . Humana Press Inc.

2009

Ortolano, F. et al. (2009) Imaging T-cell movement in the brain during experimental cerebral malaria. Parasite Immunology, 31 (3). 147-150 (4). ISSN 0141-9838 (doi:10.1111/j.1365-3024.2008.01090.x)

Jongbloed, S.L., Benson, R. , Nickdel, M.B. , Garside, P. , McInnes, I. , and Brewer, J.M. (2009) Plasmacytoid dendritic cells regulate breach of self-tolerance in autoimmune arthritis. Journal of Immunology, 182 (2). pp. 963-968. ISSN 0022-1767

Dever, G., Rodolico, G., Berrino, L., McInnes, I. , Brewer, J. , Garside, P. , and Maffia, P. (2009) Three dimensional imaging of atherosclerotic plaques in apolipoprotien e-deficient mice. In: Spring Meeting of the British-Society-for-Cardiovascular-Research 2009, 02-04 Apr 2009, Oxford, Uk.

Dever, G., Rodolico , G., Liberato, B., McInnes, I. , Brewer, J. , Garside, P. , and Maffia, P. (2009) Confocal and multiphoton laser scanning microscopy provide 3-dimensional imaging of atherosclerotic plaques in apolipoprotein E-deficient mice. In: Arteriosclerosis, Thrombosis and Vascular Biology Annual Conference 2009, 29 Apr - 1 May 2009, Washington DC, USA.

Hutchison, S., Choo-Kang, B.S.W., Gibson, V.B., Bundick, R.V., Leishman, A.J., Brewer, J.M. , McInnes, I. , and Garside, P. (2009) An investigation of the impact of the location and timing of antigen-specific T cell division on airways inflammation. Clinical and experimental immunology, 155 (1). pp. 107-116. ISSN 0009-9104

Nickdel, M.B., Conigliaro, P. , Valesini, G., Hutchison, S. , Benson, R. , Bundick, R.V., Leishman, A.J., McInnes, I. , Brewer, J.M. , and Garside, P. (2009) Dissecting the contribution of innate and antigen-specific pathways to the breach of self-tolerance observed in a murine model of arthritis. Annals of the Rheumatic Diseases, 68 (6). pp. 1059-1066. ISSN 0003-4967 (doi:10.1136/ard.2008.089300)

Rush, C.M., Millington, O.R. , Hutchison, S. , Bryson, K. , Brewer, J.M. , and Garside, P. (2009) Characterization of CD4+ T-cell-dendritic cell interactions during secondary antigen exposure in tolerance and priming. Immunology, 128 (4). pp. 463-471. ISSN 0019-2805 (doi:10.1111/j.1365-2567.2009.03124.x)

2008

Culshaw, SE, Millington, OR , Brewer, JM , and McInnes, I (2008) Murine neutrophils present Class II restricted antigen. Immunology Letters, 118 (1). pp. 49-54. ISSN 0165-2478 (doi:10.1016/j.imlet.2008.02.008)

Hutchison, S., Choo-Kang, B.S.W., Bundick, R.V., Leishman, A.J., Brewer, J.M. , McInnes, I. , and Garside, P. (2008) Tumour necrosis factor-alpha blockade suppresses murine allergic airways inflammation. Clinical and experimental immunology, 151 (1). pp. 114-122. ISSN 0009-9104

2007

Maffia, P., Zinselmeyer, B.H. , Ialenti, A., Kennedy, S. , Baker, A.H. , McInnes, I. , Brewer, J.M. , and Garside, P. (2007) Multiphoton microscopy for 3-dimensional Imaging of lymphocyte recruitment into apolipoprotein-E-deficient mouse carotid artery. Circulation, 115 (11). E326-E328.

Millington, OR, Gibson, VB, Rush, CM, Zinselmeyer, BH, Phillips, RS , Garside, P , and Brewer, JM (2007) Malaria impairs T cell clustering and immune priming despite normal signal 1 from dendritic cells. Plos Pathogens, 3 . pp. 1380-1387. (doi:10.1371/journal.ppat.0030143)

Trif, M, Roseanu, A, Brock, JH , and Brewer, JM (2007) Designing lipid nanostructures for local delivery of biologically active macromolecules. Journal of Liposome Research, 17 . pp. 237-248. (doi:10.1080/08982100701530027)

2006

Brewer, JM (2006) (How) do aluminium adjuvants work? Immunology Letters, 102 . pp. 10-15. (doi:10.1016/j.imlet.2005.08.002)

McDonald, F, Mohrs, M, and Brewer, J (2006) Using bicistronic IL-4 reporter mice to identify IL-4 expressing cells following immunisation with aluminium adjuvant. Vaccine, 24 . pp. 5393-5399. ISSN 0264-410X (doi:10.1016/j.vaccine.2006.03.049)

Schneider, H, Downey, J, Smith, A , Zinselmeyer, BH, Rush, C, Brewer, JM , Wei, B, Hogg, N, Garside, P , and Rudd, CE (2006) Reversal of the TCR stop signal by CTLA-4. Science, 313 . pp. 1972-1975. ISSN 0036-8075 (doi:10.1126/science.1131078)

Smith, KM, Garside, P , Mcneil, RC, and Brewer, JM (2006) Analysis of costimulatory molecule expression on antigen-specific T and B cells during the induction of adjuvant-induced Th1 and Th2 type responses. Vaccine, 24 . pp. 3035-3043. ISSN 0264-410X (doi:10.1016/j.vaccine.2005.10.027)

2005

Choo-Kang, BSW, Hutchison, S, Nickdel, MB, Bundick, RV, Leishman, AJ, Brewer, JM , McInnes, IB , and Garside, P (2005) TNF-blocking therapies: an alternative mode of action? Trends in Immunology, 26 . pp. 518-522. (doi:10.1016/j.it.2005.07.007)

Grierson, AM, Mitchell, P, Adams, CL, Mowat, AM , Brewer, JM , Harnett, MM , and Garside, P (2005) Direct quantitation of T cell signaling by laser scanning cytometry. Journal of Immunological Methods, 301 . pp. 140-153. (doi:10.1016/j.jim.2005.04.011)

Zinselmeyer, B. H. et al. (2005) In situ characterization of CD4+ T cell behavior in mucosal and systemic lymphoid tissues during the induction of oral priming and tolerance. Journal of Experimental Medicine, 201 (11). pp. 1815-1823. ISSN 0022-1007 (doi:10.1084/jem.20050203)

Zinselmeyer, BH et al. (2005) In situ characterization of CD4(+) T cell behavior in mucosal and systemic lymphoid tissues during the induction of oral priming and tolerance. Journal of Experimental Medicine, 201 . pp. 1815-1823. (doi:10.1084/jem.20050203)

2004

Brewer, JM, Pollock, KGJ, Tetley, L, and Russell, DG (2004) Vesicle size influences the trafficking, processing, and presentation of antigens in lipid vesicles. Journal of Immunology, 173 . pp. 6143-6150.

Maffia, P, Brewer, JM , Gracie, JA, Ianaro, A, Leung, BP , Mitchell, PJ, Smith, KM, McInnes, LB, and Garside, P (2004) Inducing experimental arthritis and breaking self-tolerance, to joint-specific antigens with trackable, ovalbumin-specific T cells. Journal of Immunology, 173 . pp. 151-156.

Smith, KM, Brewer, JM , Mowat, AM , Ron, Y, and Garside, P (2004) The influence of follicular migration on T-cell differentiation. Immunology, 111 . pp. 248-251. (doi:10.1111/j.1365-2567.2004.01813.x)

Smith, KM, Brewer, JM , Rush, CM, Riley, J, and Garside, P (2004) In vivo generated Th1 cells can migrate to B cell follicles to support B cell responses. Journal of Immunology, 173 . pp. 1640-1646.

Wei, XQ, Niedbala, W , Xu, DM , Luo, ZX, Pollock, KGJ, and Brewer, JM (2004) Host genetic background determines whether IL-18 deficiency results in increased susceptibility or resistance to murine Leishmania major infection. Immunology Letters, 94 . pp. 35-37. (doi:10.1016/j.imlet.2004.04.001)

2003

Pollock, KGJ, Conacher, M, Wei, XQ, Alexander, J, and Brewer, JM (2003) Interleukin-18 plays a role in both the alum-induced T helper 2 response and the T helper 1 response induced by alum-adsorbed interleukin-12. Immunology, 108 . pp. 137-143.

Pollock, KGJ, Mcneil, KS, Mottram, JC , Lyons, RE, Brewer, JM , Scott, P , Coombs, GH , and Alexander, J (2003) The Leishmania mexicana cysteine protease, CPB2.8, induces potent Th2 responses. Journal of Immunology, 170 . pp. 1746-1753.

Smith, KM, Brewer, JM , Webb, P, Coyle, AJ, Gutierrez-Ramos, C, and Garside, P (2003) Inducible costimulatory molecule-B7-related protein 1 interactions are important for the clonal expansion and B cell helper functions of naive, Th1, and Th2 T cells. Journal of Immunology, 170 . pp. 2310-2315.

Sun, HF, Pollock, KGJ, and Brewer, JM (2003) Analysis of the role of vaccine adjuvants in modulating dendritic cell activation and antigen presentation in vitro. Vaccine, 21 . pp. 849-855.

2002

Leung, BP, Conacher, M, Hunter, D, McInnes, IB , Liew, FY , and Brewer, JM (2002) A novel dendritic cell-induced model of erosive inflammatory arthritis: Distinct roles for dendritic cells in T cell activation and induction of local inflammation. Journal of Immunology, 169 . pp. 7071-7077.

2001

Conacher, M, Alexander, J, and Brewer, JM (2001) Oral immunisation with peptide and protein antigens by formulation in lipid vesicles incorporating bile salts (bilosomes). Vaccine, 19 . pp. 2965-2974.

Trif, M, Guillen, C, Vaughan, DM, Telfer, JM, Brewer, JM , Roseanu, A, and Brock, JH (2001) Liposomes as possible carriers for lactoferrin in the local treatment of inflammatory diseases. Experimental Biology and Medicine, 226 . pp. 559-564.

This list was generated on Thu May 24 12:02:00 2012 BST.
  • Understanding and manipulating chemokines and their receptors in the context of adjuvants
    BBSRC
    2011 - 2014
     
  • Imaging immune cells in lung in order to understand asthma
    Asthma-UK
    2011 - 2012
     
  • BTCure
    European Commission
    2011 - 2016
     
  • BTCure
    European Commission
    2011 - 2016
     
  • Targeting cell contact to reduce autoimmunity and enhance vaccination
    Scottish Universities Life Sciences Alliance
    2011 - 2012
     
  • Defining the parasitological and immunological basis of cerebral pathology during murine experimental cerebral malaria
    MRC
    2010 - 2012
     
  • Defining the parasitological and immunological basis of cerebral pathology during murine experimental cerebral malaria
    London School of Hygiene and Tropical Medicine
    2010 - 2012
     
  • In Vivo Reporting using Nanosystems Chemistry and Optical Spectroscopy
    EPSRC
    2010 - 2011
     
  • Visualising antigen presentation in mouse models of atherosclerosis
    Medical Research Scotland
    2010 - 2012
     
  • Investigating the role of T cells in vascular pathology
    British Heart Foundation
    2010 - 2010
     
  • In vivo imaging models of African trypanosomiasis to support drug discovery programmes.
    Gates Foundation
    2010 - 2012
     
  • Investigating the contributions of antigen targeting and inflammation to adjuvant function
    Wellcome Trust
    2009 - 2012
     
  • Investigating the contributions of inflammation and antigen depot to adjuvant function in vivo
    BBSRC
    2009 - 2010
     
  • Dissecting the adaptive immune response to cariogenic bacteria in the oral cavity
    Medical Research Scotland
    2009 - 2010
     
  • International Network in Theoretical Immunology (INTI)
    European Commission
    2009 - 2012
     
  • Dissecting the roles played by Dendritic cells and T lymphocytes in mediating inflammatory arthropathies
    Scottish Executive Health Department
    2005 - 2008
     
  • Analysis of the role of cholestrol-sensitive domains in the trafficking and neurotoxicity of prions
    Tenovus-Scotland
    2004 - 2005
     
  • Elucidating immune mechanisms in inflammatory arthritis
    The Nuffield Foundation
    2004 - 2013
     
  • Revealing the cellular and molecular interactions underlying the development of tolerance or immunity in real time in vivo
    Wellcome Trust
    2003 - 2005
     
  • Dendritic cell function and immune responsiveness in malaria infections
    Wellcome Trust
    2002 - 2005
     
  • Characterising the role of APC phenotype and activation state in the induction of Th1/Th2 responses in vivo
    BBSRC
    2001 - 2005