Dr Eva Gluenz

Affiliate (School of Infection & Immunity)

Wellcome Centre for Integrative Parasitology, Institute of Infection, Immunity and Inflammation, Sir Graeme Davies Building

Import to contacts

https://orcid.org/0000-0003-4346-8896

Research interests

I am a microbiologist with a longstanding interest in the biology of kinetoplastids, protozoan parasites that cause neglected diseases in humans and animals. My goal is to elucidate basic mechanisms of disease through the study of parasite cell biology. To harness genome, transcriptome and proteome data we developed CRISPR/Cas9 editing tools for kinetoplastids, which allow for rapid and precise modification of individual genes as well as large scale loss-of-function screens.

Current research projects

Contribution of membrane transporters to parasite fitness

Membrane transporters or channels act as gatekeepers to determine which substances enter or exit the cell. Possessing the correct set of transporters is vital for the parasite in each of its life cycle stages, to grow in a sand fly and to exploit its host cell, the macrophage, effectively. We are screening a library of membrane transporter mutants to discover which transporters are critical for survival in macrophages, what their functions are and whether they contribute to drug action.

Structure and function of the Leishmania flagellum

Eukaryotic flagella are conserved organelles with functions ranging from motility to cell adhesion and sensing. By studying the Leishmania flagellum we aim to gain insight into molecular mechanisms involved in the formation of a functional motile flagellum. We are currently using gene deletion libraries to study the functions of individual proteins and the role of cAMP signalling in the regulation of motility. We are also using pooled loss-of-function screens of a wider cohort of genes to discover their importance in parasite transmission and infection.

Publications

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Number of items: 42.

2023

McCoy, C. J., Paupelin-Vaucelle, H., Gorilak, P., Beneke, T., Varga, V. and Gluenz, E. (2023) ULK4 and Fused/STK36 interact to mediate assembly of a motile flagellum. Molecular Biology of the Cell, 34(7), ar66. (doi: 10.1091/mbc.E22-06-0222) (PMID:36989043) (PMCID:PMC10295485)

2021

Espada, C. R., Quilles Jr., J. C., Albuquerque-Wendt, A. , Cruz, M. C., Beneke, T., Lorenzon, L. B., Gluenz, E. , Cruz, A. K. and Uliana, S. R.B. (2021) Effective genome editing in Leishmania (Viannia) braziliensis stably expressing Cas9 and T7 RNA polymerase. Frontiers in Cellular and Infection Microbiology, 11, 772311. (doi: 10.3389/fcimb.2021.772311) (PMID:34858879) (PMCID:PMC8631273)

Espada, C. R., Albuquerque-Wendt, A. , Hornillos, V., Gluenz, E. , Coelho, A. C. and Uliana, S. R.B. (2021) Ros3 (Lem3p/CDC50) gene dosage is implicated in miltefosine susceptibility in Leishmania (Viannia) braziliensis clinical isolates and in Leishmania (Leishmania) major. ACS Infectious Diseases, 7(4), pp. 849-858. (doi: 10.1021/acsinfecdis.0c00857) (PMID:33724800) (PMCID:PMC8042657)

2020

Beneke, T., Banecki, K., Fochler, S. and Gluenz, E. (2020) LAX28 is required for the stable assembly of the inner dynein arm f complex, and the tether and tether head complex in Leishmania flagella. Journal of Cell Science, 133(2), jcs239855. (doi: 10.1242/jcs.239855) (PMID:31932510)

2019

Schädeli, D., Serricchio, M., Ben Hamidane, H., Loffreda, A., Hemphill, A., Beneke, T., Gluenz, E. , Graumann, J. and Bütikofer, P. (2019) Cardiolipin depletion–induced changes in the Trypanosoma brucei proteome. FASEB Journal, 33(12), pp. 13161-13175. (doi: 10.1096/fj.201901184RR) (PMID:31536395)

Beneke, T. et al. (2019) Genetic dissection of a Leishmania flagellar proteome demonstrates requirement for directional motility in sand fly infections. PLoS Pathogens, 15(6), e1007828. (doi: 10.1371/journal.ppat.1007828) (PMID:31242261) (PMCID:PMC6615630)

Beneke, T. and Gluenz, E. (2019) LeishGEdit: a method for rapid gene knockout and tagging using CRISPR-Cas9. In: Leishmania. Series: Methods in molecular biology, 1971. Humana Press: New York, pp. 189-210. ISBN 9781493992102 (doi: 10.1007/978-1-4939-9210-2_9)

2018

Ooi, C.-P., Smith, T. K., Gluenz, E. , Wand, N. V., Vaughan, S. and Rudenko, G. (2018) Blocking variant surface glycoprotein synthesis alters endoplasmic reticulum exit sites/Golgi homeostasis in Trypanosoma brucei. Traffic, 19(6), pp. 391-405. (doi: 10.1111/tra.12561)

Costa, F. C. et al. (2018) Expanding the toolbox for Trypanosoma cruzi: a parasite line incorporating a bioluminescence-fluorescence dual reporter and streamlined CRISPR/Cas9 functionality for rapid in vivo localisation and phenotyping. PLoS Neglected Tropical Diseases, 12(4), e0006388. (doi: 10.1371/journal.pntd.0006388) (PMID:29608569) (PMCID:PMC5897030)

2017

Martel, D., Beneke, T., Gluenz, E. , Späth, G. F. and Rachidi, N. (2017) Characterisation of casein kinase 1.1 in Leishmania donovani using the CRISPR Cas9 toolkit. BioMed Research International, 2017, 4635605. (doi: 10.1155/2017/4635605) (PMID:29333442) (PMCID:PMC5733176)

Beneke, T., Madden, R., Makin, L., Valli, J., Sunter, J. and Gluenz, E. (2017) A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids. Royal Society Open Science, 4(5), 170095. (doi: 10.1098/rsos.170095) (PMID:28573017) (PMCID:PMC5451818)

2016

Sanchez, M. A., Tran, K. D., Valli, J., Hobbs, S., Johnson, E., Gluenz, E. and Landfear, S. M. (2016) KHARON Is an essential cytoskeletal protein involved in the trafficking of flagellar membrane proteins and cell division in African trypanosomes. Journal of Biological Chemistry, 291(38), pp. 19760-19773. (doi: 10.1074/jbc.M116.739235) (PMID:27489106) (PMCID:PMC5025667)

2015

Wheeler, R.J., Gluenz, E. and Gull, K. (2015) Basal body multipotency and axonemal remodelling are two pathways to a 9+0 flagellum. Nature Communications, 6, 8964. (doi: 10.1038/ncomms9964) (PMID:26667778) (PMCID:PMC4682162)

Fiebig, M., Kelly, S. and Gluenz, E. (2015) Comparative life cycle transcriptomics revises Leishmania mexicana genome annotation and links a chromosome duplication with parasitism of vertebrates. PLoS Pathogens, 11(10), e1005186. (doi: 10.1371/journal.ppat.1005186)

Blader, I. J., Tran, K. D., Vieira, D. P., Sanchez, M. A., Valli, J., Gluenz, E. and Landfear, S. M. (2015) Kharon1 null mutants of Leishmania mexicana are avirulent in mice and exhibit a cytokinesis defect within macrophages. PLoS ONE, 10(8), e0134432. (doi: 10.1371/journal.pone.0134432) (PMID:26266938) (PMCID:PMC4534133)

Makin, L. and Gluenz, E. (2015) cAMP signalling in trypanosomatids: role in pathogenesis and as a drug target. Trends in Parasitology, 31(8), pp. 373-379. (doi: 10.1016/j.pt.2015.04.014) (PMID:26004537) (PMCID:PMC4534343)

Wheeler, R., Gull, K. and Gluenz, E. (2015) 9+2 to 9+0 axoneme conversion in Leishmania. Cilia, 4, O5. (doi: 10.1186/2046-2530-4-S1-O5)

Gluenz, E. , Wheeler, R. J., Hughes, L. and Vaughan, S. (2015) Scanning and three-dimensional electron microscopy methods for the study of Trypanosoma brucei and Leishmania mexicana flagella. In: Methods in Cilia and Flagella. Series: Methods in cell biology, 127. Elsevier, pp. 509-542. (doi: 10.1016/bs.mcb.2014.12.011)

Dean, S., Sunter, J., Wheeler, R. J., Hodkinson, I., Gluenz, E. and Gull, K. (2015) A toolkit enabling efficient, scalable and reproducible gene tagging in trypanosomatids. Open Biology, 5(1), 140197. (doi: 10.1098/rsob.140197) (PMID:25567099) (PMCID:PMC4313374)

2014

Fiebig, M., Gluenz, E. , Carrington, M. and Kelly, S. (2014) SLaP mapper: a webserver for identifying and quantifying spliced-leader addition and polyadenylation site usage in kinetoplastid genomes. Molecular and Biochemical Parasitology, 196(2), pp. 71-74. (doi: 10.1016/j.molbiopara.2014.07.012) (PMID:25111964) (PMCID:PMC4222701)

2013

Wheeler, R. J., Gluenz, E. and Gull, K. (2013) The limits on Trypanosomatid morphological diversity. PLoS ONE, 8(11), e79581. (doi: 10.1371/journal.pone.0079581) (PMID:24260255) (PMCID:PMC3834336)

Povelones, M. L., Tiengwe, C., Gluenz, E. , Gull, K., Englund, P. T. and Jensen, R. E. (2013) Mitochondrial shape and function in trypanosomes requires the outer membrane protein, TbLOK1. Molecular Microbiology, 87(4), pp. 713-729. (doi: 10.1111/mmi.12089) (PMID:23336702) (PMCID:PMC4715881)

2012

Povelones, M. L., Gluenz, E. , Dembek, M., Gull, K. and Rudenko, G. (2012) Histone H1 plays a role in heterochromatin formation and VSG expression site silencing in Trypanosoma brucei. PLoS Pathogens, 8(11), e1003010. (doi: 10.1371/journal.ppat.1003010) (PMID:23133390) (PMCID:PMC3486875)

Wheeler, R. J., Gull, K. and Gluenz, E. (2012) Detailed interrogation of trypanosome cell biology via differential organelle staining and automated image analysis. BMC Biology, 10, 1. (doi: 10.1186/1741-7007-10-1) (PMID:22214525) (PMCID:PMC3398262)

2011

Gluenz, E. , Barker, A. R. and Gull, K. (2011) An expanded family of proteins with BPI/LBP/PLUNC-like domains in trypanosome parasites: an association with pathogenicity? Biochemical Society Transactions, 39(4), pp. 966-970. (doi: 10.1042/BST0390966) (PMID:21787331)

Clayton, A. M., Guler, J. L., Povelones, M. L., Gluenz, E. , Gull, K., Smith, T. K., Jensen, R. E. and Englund, P. T. (2011) Depletion of mitochondrial acyl carrier protein in bloodstream-form Trypanosoma brucei causes a kinetoplast segregation defect. Eukaryotic Cell, 10(3), pp. 286-292. (doi: 10.1128/EC.00290-10) (PMID:21239625) (PMCID:PMC3067480)

Gluenz, E. , Povelones, M. L., Englund, P. T. and Gull, K. (2011) The kinetoplast duplication cycle in Trypanosoma brucei is orchestrated by cytoskeleton-mediated cell morphogenesis. Molecular and Cellular Biology, 31(5), pp. 1012-1021. (doi: 10.1128/MCB.01176-10) (PMID:21173163) (PMCID:PMC3067821)

Wheeler, R. J., Gluenz, E. and Gull, K. (2011) The cell cycle of Leishmania: morphogenetic events and their implications for parasite biology. Molecular Microbiology, 79(3), pp. 647-662. (doi: 10.1111/j.1365-2958.2010.07479.x) (PMID:21255109) (PMCID:PMC3166656)

2010

Wickstead, B., Carrington, J. T., Gluenz, E. and Gull, K. (2010) The expanded Kinesin-13 repertoire of trypanosomes contains only one mitotic Kinesin indicating multiple extra-nuclear roles. PLoS ONE, 5(11), e15020. (doi: 10.1371/journal.pone.0015020) (PMID:21124853) (PMCID:PMC2990766)

Gluenz, E. , Höög, J. L., Smith, A. E., Dawe, H. R., Shaw, M. K. and Gull, K. (2010) Beyond 9+0: noncanonical axoneme structures characterize sensory cilia from protists to humans. FASEB Journal, 24(9), pp. 3117-3121. (doi: 10.1096/fj.09-151381) (PMID:20371625) (PMCID:PMC2923350)

Gluenz, E. , Ginger, M. L. and McKean, P. G. (2010) Flagellum assembly and function during the Leishmania life cycle. Current Opinion in Microbiology, 13(4), pp. 473-479. (doi: 10.1016/j.mib.2010.05.008) (PMID:20541962)

Höög, J. L., Gluenz, E. , Vaughan, S. and Gull, K. (2010) Ultrastructural investigation methods for Trypanosoma brucei. Methods in Cell Biology, 96, pp. 175-196. (doi: 10.1016/S0091-679X(10)96008-1) (PMID:20869523)

2009

Sharma, R., Gluenz, E. , Peacock, L., Gibson, W., Gull, K. and Carrington, M. (2009) The heart of darkness: Growth and form of Trypanosoma brucei in the tsetse fly. Trends in Parasitology, 25(11), pp. 517-524. (doi: 10.1016/j.pt.2009.08.001) (PMID:19747880) (PMCID:PMC3770903)

Smith, T. K., Vasileva, N., Gluenz, E. , Terry, S., Portman, N., Kramer, S., Carrington, M., Michaeli, S., Gull, K. and Rudenko, G. (2009) Blocking variant surface Glycoprotein synthesis in Trypanosoma brucei triggers a general arrest in translation initiation. PLoS ONE, 4(10), e7532. (doi: 10.1371/journal.pone.0007532) (PMID:19855834) (PMCID:PMC2762041)

Signorell, A., Gluenz, E. , Rettig, J., Schneider, A., Shaw, M. K., Gull, K. and Bütikofer, P. (2009) Perturbation of phosphatidylethanolamine synthesis affects mitochondrial morphology and cell-cycle progression in procyclic-form Trypanosoma brucei. Molecular Microbiology, 72(4), pp. 1068-1079. (doi: 10.1111/j.1365-2958.2009.06713.x) (PMID:19400804)

Dawe, H. and Gluenz, E. (2009) The tale of the trypanosome tail. Biologist, 56(4), pp. 216-220.

2008

Barker, A. R., Wickstead, B., Gluenz, E. and Gull, K. (2008) Bioinformatic insights to the ESAG5 and GRESAG5 gene families in kinetoplastid parasites. Molecular and Biochemical Parasitology, 162(2), pp. 112-122. (doi: 10.1016/j.molbiopara.2008.08.003) (PMID:18773926)

Lindsay, M. E., Gluenz, E. , Gull, K. and Englund, P. T. (2008) A new function of Trypanosoma brucei mitochondrial topoisomerase II is to maintain kinetoplast DNA network topology. Molecular Microbiology, 70(6), pp. 1465-1476. (doi: 10.1111/j.1365-2958.2008.06493.x) (PMID:19019151) (PMCID:PMC2993328)

Gluenz, E. , Sharma, R., Carrington, M. and Gull, K. (2008) Functional characterization of cohesin subunit SCC1 in Trypanosoma brucei and dissection of mutant phenotypes in two life cycle stages. Molecular Microbiology, 69(3), pp. 666-680. (doi: 10.1111/j.1365-2958.2008.06320.x) (PMID:18554326) (PMCID:PMC2610385)

Sharma, R., Peacock, L., Gluenz, E. , Gull, K., Gibson, W. and Carrington, M. (2008) Asymmetric cell division as a route to reduction in cell length and change in cell morphology in trypanosomes. Protist, 159(1), pp. 137-151. (doi: 10.1016/j.protis.2007.07.004) (PMID:17931969)

2007

Gluenz, E. , Shaw, M. K. and Gull, K. (2007) Structural asymmetry and discrete nucleic acid subdomains in the Trypanosoma brucei kinetoplast. Molecular Microbiology, 64(6), pp. 1529-1539. (doi: 10.1111/j.1365-2958.2007.05749.x) (PMID:17511811) (PMCID:PMC1974780)

Gluenz, E. , Taylor, M. C. and Kelly, J. M. (2007) The Trypanosoma cruzi metacyclic-specific protein Met-III associates with the nucleolus and contains independent amino and carboxyl terminal targeting elements. International Journal for Parasitology, 37(6), pp. 617-625. (doi: 10.1016/j.ijpara.2006.11.016) (PMID:17239886) (PMCID:PMC2424140)

This list was generated on Mon Apr 22 15:25:02 2024 BST.

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