Institute of Infection, Immunity and Inflammation

Prof David Smith

  • Professor (Bacteriology)
  • Associate Academic (School of Veterinary Medicine)

email: David.G.Smith@glasgow.ac.uk


Epithelial surfaces represent critical sites of interaction with host species for many bacterial pathogens. Our research deals with a range of bacterial pathogens (largely enteric), taking multi-disciplinary approaches to understand molecular determinants of infectivity and pathogenicity.

Lawsonia intracellularis is an obligate intracellular pathogen of pigs that induces hyperplasia of infected intestinal epithelium. We reported (McCluskey et al., 2002) the first specified gene product- LsaA related to pathogenicity. To date, this paper remains the sole example of work taking a molecular approach to define Lawsonia virulence and similar investigations, now incorporating genomics and proteomics, are continuing.

E. coli O157:H7 is an important foodborne pathogen and determinants that predicate asymptomatic infection in reservoir hosts such as cattle are objectives. Among these are bacterial surface (Roe et al., 2004) and secreted (Hoey et al. 2003) factors. To investigate these interactions, we developed a primary bovine epithelial cell culture system that has been used for additional studies to model interactions between these bacteria and cattle. More recently, investigations have employed proteomics. A significant advance was the development of an efficient methodology (Batycka et al., 2006) to study prokaryotic and eukaryotic systems that, along with other proteomics methods, is now employed to advance the understanding of numerous pathogens.

List all by: Type | Date

Jump to: 2013 | 2012 | 2011 | 2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2001
Number of items: 27.

2013

Gilchrist, T.L., Smith, D.G.E., Fitzpatrick, J.L., Zadoks, R.N., and Fontaine, M.C. (2013) Comparative molecular analysis of ovine and bovine Streptococcus uberis isolates. Journal of Dairy Science, 96 (2). pp. 962-970. ISSN 0022-0302 (doi:10.3168/jds.2012-5705)

2012

Elmi, A. et al. (2012) Campylobacter jejuni outer membrane vesicles play an important role in bacterial interactions with human intestinal epithelial cells. Infection and Immunity, 80 (12). pp. 4089-4098. ISSN 0019-9567 (doi:10.1128/IAI.00161-12)

Wheelhouse, N., Sait, M., Wilson, K., Aitchison, K., McLean, K., Smith, D.G.E., and Longbottom, D. (2012) Expression patterns of five polymorphic membrane proteins during the Chlamydia abortus developmental cycle. Veterinary Microbiology, 160 (3-4). pp. 525-529. ISSN 0378-1135 (doi:10.1016/j.vetmic.2012.06.017)

Pethick, F.E. et al. (2012) Complete genome sequences of corynebacterium pseudotuberculosis strains 3/99-5 and 42/02-A, isolated from sheep in Scotland and Australia, respectively. Journal of Bacteriology, 194 (17). pp. 4736-4737. ISSN 0021-9193 (doi:10.1128/JB.00918-12)

Pethick, F.E. et al. (2012) Complete genome sequence of Corynebacterium pseudotuberculosis strain 1/06-A, isolated from a horse in North America. Journal of Bacteriology, 194 (16). p. 4476. ISSN 0021-9193 (doi:10.1128/JB.00922-12)

Wheelhouse, N.M., Sait, M., Aitchison, K., Livingstone, M., Wright, F., McLean, K., Inglis, N.F., Smith, D.G.E., and Longbottom, D. (2012) Processing of chlamydia abortus polymorphic membrane protein 18D during the chlamydial developmental cycle. PLoS ONE, 7 (11). e49190. ISSN 1932-6203 (doi:10.1371/journal.pone.0049190)

2011

Fraser-Pitt, D.J., Cameron, P., McNeilly, T.N., Boyd, A., Manson, E.D.T., and Smith, D.G.E. (2011) Phosphorylation of the epidermal growth factor receptor (EGFR) is essential for interleukin-8 release from intestinal epithelial cells in response to challenge with Escherichia coli O157 : H7 flagellin. Microbiology, 157 (8). pp. 2339-2347. ISSN 1350-0872 (doi:10.1099/mic.0.047670-0)

Sait, M., Clark, E.M., Wheelhouse, N., Livingstone, M., Spalding, L., Siarkou, V.I., Vretou, E., Smith, D.G.E., Lainson, F.A., and Longbottom, D. (2011) Genome sequence of the Chlamydophila abortus variant strain LLG. Journal of Bacteriology, 193 (16). pp. 4276-4277. ISSN 0021-9193 (doi:10.1128/JB.05290-11)

Sait, M. et al. (2011) Genetic variability of Chlamydophila abortus strains assessed by PCR-RFLP analysis of polymorphic membrane protein-encoding genes. Veterinary Microbiology, 151 (3-4). pp. 284-290. ISSN 0378-1135 (doi:10.1016/j.vetmic.2011.03.005)

Watson, E., Clark, E.M., Alberdi, M.P., Inglis, N.F., Porter, M., Imrie, L., Mclean, K., Manson, E., Lainson, A., and Smith, D.G.E. (2011) A novel Lawsonia intracellularis autotransporter protein is a prominent antigen. Clinical and Vaccine Immunology, 18 (8). pp. 1282-1287. ISSN 1556-6811 (doi:10.1128/CVI.05073-11)

Bannoehr, J. et al. (2011) Genomic and Surface Proteomic Analysis of the Canine Pathogen Staphylococcus pseudintermedius Reveals Proteins That Mediate Adherence to the Extracellular Matrix. Infection and Immunity, 79 (8). pp. 3074-3086. ISSN 0019-9567 (doi:10.1128/IAI.00137-11)

Sherry, A.E., Inglis, N.F., Stevenson, A., Fraser-Pitt, D.G.E., Everest, P., Smith, David, and Roberts, M. (2011) Characterisation of proteins extracted from the surface of Salmonella Typhimurium grown under SPI-2-inducing conditions by LC-ESI/MS/MS sequencing. Proteomics, 11 (3). pp. 361-370. ISSN 1615-9853 (doi:10.1002/pmic.200900802)

Walker, C. A., Donachie, W., Smith, D.G.E., and Fontaine, M.C. (2011) Targeted allele-replacement mutagenesis of Corynebacterium pseudotuberculosis. Applied and Environmental Microbiology, 77 (10). pp. 3532-3535. ISSN 0099-2240 (doi:10.1128/AEM.01740-10)

Watson, E., Clark, E.M., Alberdi, M.P., Inglis, N.F., Porter, M., Imrie, L., Mclean, K., Manson, E., Lainson, A., and Smith, D.G.E. (2011) A novel Lawsonia intracellularis autotransporter protein is a prominent antigen. Clinical and Vaccine Immunology, 18 (8). pp. 1282-1287. ISSN 1556-6811 (doi:10.1128/CVI.05073-11)

2010

McNeilly, T.N. et al. (2010) IgA and IgG antibody responses following systemic immunization of cattle with native H7 flagellin differ in epitope recognition and capacity to neutralise TLR5 signalling. Vaccine, 28 (5). pp. 1412-1421. ISSN 0264-410X (doi:10.1016/j.vaccine.2009.10.148)

McNeilly, T.N., Mitchell, M.C., Rosser, T., McAteer, S., Low, J.C., Smith, D.G.E., Huntley, J.F., Mahajan, A., and Gally, D.L. (2010) Immunization of cattle with a combination of purified intimin-531, EspA and Tir significantly reduces shedding of Escherichia coli O157:H7 following oral challenge. Vaccine, 28 (5). pp. 1422-1428. ISSN 0264-410X (doi:10.1016/j.vaccine.2009.10.076)

2009

Mahajan, A. et al. (2009) An investigation of the expression and adhesin function of H7 flagella in the interaction of Escherichia coli O157 : H7 with bovine intestinal epithelium. Cellular Microbiology, 11 (1). pp. 121-137. ISSN 1462-5814 (doi:10.1111/j.1462-5822.2008.01244.x)

Alberdi, M.P., Watson, E., McAllister, G.E.M., Harris, J.D., Paxton, E.A., Thomson, J.R., and Smith, D.G.E. (2009) Expression by Lawsonia intracellularis of type III secretion system components during infection. Veterinary Microbiology, 139 (3-4). pp. 298-303. ISSN 0378-1135 (doi:10.1016/j.vetmic.2009.06.022)

Mahajan, A. et al. (2009) An investigation of the expression and adhesin function of H7 flagella in the interaction of Escherichia coli O157: H7 with bovine intestinal epithelium. Cellular Microbiology, 11 (1). pp. 121-137. ISSN 1462-5814 (doi:10.1111/j.1462-5822.2008.01244.x )

2008

McNeilly, T.N., Naylor, S.W., Mahajan, A., Mitchell, M.C., McAteer, S., Deane, D., Smith, D.G.E, Low, J.C., Gally, D.L., and Huntley, J. F. (2008) Escherichia coli O157 : H7 colonization in cattle following systemic and mucosal immunization with purified H7 flagellin. Infection and Immunity, 76 (6). pp. 2594-2602. ISSN 0019-9567 (doi:10.1128/IAI.01452-07)

2007

Dziva, F, Mahajan, A, Cameron, P, Currie, C, McKendrick, IJ, Wallis, TS, Smith, DGE, and Stevens, MP (2007) EspP, a Type V-secreted serine protease of enterohaemorrhagic Escherichia coli O157 : H7, influences intestinal colonization of calves and adherence to bovine primary intestinal epithelial cells. Fems Microbiology Letters, 271 . pp. 258-264. (doi:10.1111/j.1574-6968.2007.00724.x)

McNeilly, TN, Naylor, SW, Mitchell, MC, McAteer, S, Mahajan, A, Smith, DGE, Gally, DL, Low, JC, and Huntley, JF (2007) Simple methods for measurement of bovine mucosal antibody responses in vivo. Veterinary Immunology and Immunopathology, 118 . pp. 160-167. (doi:10.1016/j.vetimm.2007.04.016)

Roe, A.J. et al. (2007) Analysis of the expression, regulation and export of NleA-E in Escherichia coli O157 : H7. Microbiology: SGM, 153 . pp. 1350-1360. ISSN 1350-0872 (doi:10.1099/mic.0.2006/003707-0)

2006

Low, AS et al. (2006) Cloning, expression, and characterization of fimbrial operon F9 from enterohemorrhagic Escherichia coli O157 : H7. Infection and Immunity, 74 . pp. 2233-2244. (doi:10.1128/IAI.74.4.2233-2244.2006)

Low, AS, Holden, N, Rosser, T, Roe, AJ, Constantinidou, C, Hobman, JL, Smith, DGE, Low, JC, and Gally, DL (2006) Analysis of firnbrial gene clusters and their expression in enterohaernorrhagic Escherichia coli O157 : H7. Environmental Microbiology, 8 . pp. 1033-1047. (doi:10.1111/j.1462-2920.2006.00995.x)

2005

Mahajan, A, Naylor, S, Mills, AD, Low, JC, Mackellar, A, Hoey, DEE, Currie, CG, Gally, DL, Huntley, J, and Smith, DGE (2005) Phenotypic and functional characterisation of follicle-associated epithelium of rectal lymphoid tissue. Cell and Tissue Research, 321 . pp. 365-374. (doi:10.1007/s00441-005-1080-1)

2001

Ayers, E., Cameron, E., Kemp, R., Leitch, H., Mollison, A., Muir, I., Reid, H., Smith, D., and Sproat, J. (2001) Oral lesions in sheep and cattle in Dumfries and Galloway. Veterinary Record, 148 (23). pp. 720-723. ISSN 0042-4900 (doi:10.1136/vr.148.23.714)

This list was generated on Wed Jun 19 16:50:57 2013 BST.
Jump to: Article
Number of items: 27.

Article

Gilchrist, T.L., Smith, D.G.E., Fitzpatrick, J.L., Zadoks, R.N., and Fontaine, M.C. (2013) Comparative molecular analysis of ovine and bovine Streptococcus uberis isolates. Journal of Dairy Science, 96 (2). pp. 962-970. ISSN 0022-0302 (doi:10.3168/jds.2012-5705)

Elmi, A. et al. (2012) Campylobacter jejuni outer membrane vesicles play an important role in bacterial interactions with human intestinal epithelial cells. Infection and Immunity, 80 (12). pp. 4089-4098. ISSN 0019-9567 (doi:10.1128/IAI.00161-12)

Wheelhouse, N., Sait, M., Wilson, K., Aitchison, K., McLean, K., Smith, D.G.E., and Longbottom, D. (2012) Expression patterns of five polymorphic membrane proteins during the Chlamydia abortus developmental cycle. Veterinary Microbiology, 160 (3-4). pp. 525-529. ISSN 0378-1135 (doi:10.1016/j.vetmic.2012.06.017)

Pethick, F.E. et al. (2012) Complete genome sequences of corynebacterium pseudotuberculosis strains 3/99-5 and 42/02-A, isolated from sheep in Scotland and Australia, respectively. Journal of Bacteriology, 194 (17). pp. 4736-4737. ISSN 0021-9193 (doi:10.1128/JB.00918-12)

Pethick, F.E. et al. (2012) Complete genome sequence of Corynebacterium pseudotuberculosis strain 1/06-A, isolated from a horse in North America. Journal of Bacteriology, 194 (16). p. 4476. ISSN 0021-9193 (doi:10.1128/JB.00922-12)

Wheelhouse, N.M., Sait, M., Aitchison, K., Livingstone, M., Wright, F., McLean, K., Inglis, N.F., Smith, D.G.E., and Longbottom, D. (2012) Processing of chlamydia abortus polymorphic membrane protein 18D during the chlamydial developmental cycle. PLoS ONE, 7 (11). e49190. ISSN 1932-6203 (doi:10.1371/journal.pone.0049190)

Fraser-Pitt, D.J., Cameron, P., McNeilly, T.N., Boyd, A., Manson, E.D.T., and Smith, D.G.E. (2011) Phosphorylation of the epidermal growth factor receptor (EGFR) is essential for interleukin-8 release from intestinal epithelial cells in response to challenge with Escherichia coli O157 : H7 flagellin. Microbiology, 157 (8). pp. 2339-2347. ISSN 1350-0872 (doi:10.1099/mic.0.047670-0)

Sait, M., Clark, E.M., Wheelhouse, N., Livingstone, M., Spalding, L., Siarkou, V.I., Vretou, E., Smith, D.G.E., Lainson, F.A., and Longbottom, D. (2011) Genome sequence of the Chlamydophila abortus variant strain LLG. Journal of Bacteriology, 193 (16). pp. 4276-4277. ISSN 0021-9193 (doi:10.1128/JB.05290-11)

Sait, M. et al. (2011) Genetic variability of Chlamydophila abortus strains assessed by PCR-RFLP analysis of polymorphic membrane protein-encoding genes. Veterinary Microbiology, 151 (3-4). pp. 284-290. ISSN 0378-1135 (doi:10.1016/j.vetmic.2011.03.005)

Watson, E., Clark, E.M., Alberdi, M.P., Inglis, N.F., Porter, M., Imrie, L., Mclean, K., Manson, E., Lainson, A., and Smith, D.G.E. (2011) A novel Lawsonia intracellularis autotransporter protein is a prominent antigen. Clinical and Vaccine Immunology, 18 (8). pp. 1282-1287. ISSN 1556-6811 (doi:10.1128/CVI.05073-11)

Bannoehr, J. et al. (2011) Genomic and Surface Proteomic Analysis of the Canine Pathogen Staphylococcus pseudintermedius Reveals Proteins That Mediate Adherence to the Extracellular Matrix. Infection and Immunity, 79 (8). pp. 3074-3086. ISSN 0019-9567 (doi:10.1128/IAI.00137-11)

Sherry, A.E., Inglis, N.F., Stevenson, A., Fraser-Pitt, D.G.E., Everest, P., Smith, David, and Roberts, M. (2011) Characterisation of proteins extracted from the surface of Salmonella Typhimurium grown under SPI-2-inducing conditions by LC-ESI/MS/MS sequencing. Proteomics, 11 (3). pp. 361-370. ISSN 1615-9853 (doi:10.1002/pmic.200900802)

Walker, C. A., Donachie, W., Smith, D.G.E., and Fontaine, M.C. (2011) Targeted allele-replacement mutagenesis of Corynebacterium pseudotuberculosis. Applied and Environmental Microbiology, 77 (10). pp. 3532-3535. ISSN 0099-2240 (doi:10.1128/AEM.01740-10)

Watson, E., Clark, E.M., Alberdi, M.P., Inglis, N.F., Porter, M., Imrie, L., Mclean, K., Manson, E., Lainson, A., and Smith, D.G.E. (2011) A novel Lawsonia intracellularis autotransporter protein is a prominent antigen. Clinical and Vaccine Immunology, 18 (8). pp. 1282-1287. ISSN 1556-6811 (doi:10.1128/CVI.05073-11)

McNeilly, T.N. et al. (2010) IgA and IgG antibody responses following systemic immunization of cattle with native H7 flagellin differ in epitope recognition and capacity to neutralise TLR5 signalling. Vaccine, 28 (5). pp. 1412-1421. ISSN 0264-410X (doi:10.1016/j.vaccine.2009.10.148)

McNeilly, T.N., Mitchell, M.C., Rosser, T., McAteer, S., Low, J.C., Smith, D.G.E., Huntley, J.F., Mahajan, A., and Gally, D.L. (2010) Immunization of cattle with a combination of purified intimin-531, EspA and Tir significantly reduces shedding of Escherichia coli O157:H7 following oral challenge. Vaccine, 28 (5). pp. 1422-1428. ISSN 0264-410X (doi:10.1016/j.vaccine.2009.10.076)

Mahajan, A. et al. (2009) An investigation of the expression and adhesin function of H7 flagella in the interaction of Escherichia coli O157 : H7 with bovine intestinal epithelium. Cellular Microbiology, 11 (1). pp. 121-137. ISSN 1462-5814 (doi:10.1111/j.1462-5822.2008.01244.x)

Alberdi, M.P., Watson, E., McAllister, G.E.M., Harris, J.D., Paxton, E.A., Thomson, J.R., and Smith, D.G.E. (2009) Expression by Lawsonia intracellularis of type III secretion system components during infection. Veterinary Microbiology, 139 (3-4). pp. 298-303. ISSN 0378-1135 (doi:10.1016/j.vetmic.2009.06.022)

Mahajan, A. et al. (2009) An investigation of the expression and adhesin function of H7 flagella in the interaction of Escherichia coli O157: H7 with bovine intestinal epithelium. Cellular Microbiology, 11 (1). pp. 121-137. ISSN 1462-5814 (doi:10.1111/j.1462-5822.2008.01244.x )

McNeilly, T.N., Naylor, S.W., Mahajan, A., Mitchell, M.C., McAteer, S., Deane, D., Smith, D.G.E, Low, J.C., Gally, D.L., and Huntley, J. F. (2008) Escherichia coli O157 : H7 colonization in cattle following systemic and mucosal immunization with purified H7 flagellin. Infection and Immunity, 76 (6). pp. 2594-2602. ISSN 0019-9567 (doi:10.1128/IAI.01452-07)

Dziva, F, Mahajan, A, Cameron, P, Currie, C, McKendrick, IJ, Wallis, TS, Smith, DGE, and Stevens, MP (2007) EspP, a Type V-secreted serine protease of enterohaemorrhagic Escherichia coli O157 : H7, influences intestinal colonization of calves and adherence to bovine primary intestinal epithelial cells. Fems Microbiology Letters, 271 . pp. 258-264. (doi:10.1111/j.1574-6968.2007.00724.x)

McNeilly, TN, Naylor, SW, Mitchell, MC, McAteer, S, Mahajan, A, Smith, DGE, Gally, DL, Low, JC, and Huntley, JF (2007) Simple methods for measurement of bovine mucosal antibody responses in vivo. Veterinary Immunology and Immunopathology, 118 . pp. 160-167. (doi:10.1016/j.vetimm.2007.04.016)

Roe, A.J. et al. (2007) Analysis of the expression, regulation and export of NleA-E in Escherichia coli O157 : H7. Microbiology: SGM, 153 . pp. 1350-1360. ISSN 1350-0872 (doi:10.1099/mic.0.2006/003707-0)

Low, AS et al. (2006) Cloning, expression, and characterization of fimbrial operon F9 from enterohemorrhagic Escherichia coli O157 : H7. Infection and Immunity, 74 . pp. 2233-2244. (doi:10.1128/IAI.74.4.2233-2244.2006)

Low, AS, Holden, N, Rosser, T, Roe, AJ, Constantinidou, C, Hobman, JL, Smith, DGE, Low, JC, and Gally, DL (2006) Analysis of firnbrial gene clusters and their expression in enterohaernorrhagic Escherichia coli O157 : H7. Environmental Microbiology, 8 . pp. 1033-1047. (doi:10.1111/j.1462-2920.2006.00995.x)

Mahajan, A, Naylor, S, Mills, AD, Low, JC, Mackellar, A, Hoey, DEE, Currie, CG, Gally, DL, Huntley, J, and Smith, DGE (2005) Phenotypic and functional characterisation of follicle-associated epithelium of rectal lymphoid tissue. Cell and Tissue Research, 321 . pp. 365-374. (doi:10.1007/s00441-005-1080-1)

Ayers, E., Cameron, E., Kemp, R., Leitch, H., Mollison, A., Muir, I., Reid, H., Smith, D., and Sproat, J. (2001) Oral lesions in sheep and cattle in Dumfries and Galloway. Veterinary Record, 148 (23). pp. 720-723. ISSN 0042-4900 (doi:10.1136/vr.148.23.714)

This list was generated on Wed Jun 19 16:50:57 2013 BST.

Grants and Awards listed are those received whilst working with the University of Glasgow.

  • Defining the molecular basis of H7 flagellin as an adhesion and mucosal adjuvant for vaccine development
    Biotechnology and Biological Sciences Research Council
    2012 - 2015
     
  • Integrated systems approach for preventing uterine disease in dairy cattle
    BBSRC
    2011 - 2014
     
  • Developing piglet models for investigation of Campylobacter jejuni diarrhoeal disease.
    BBSRC
    2008 - 2010
     
  • Molecular and functional characterisation of a type III secretion system (TTSS) of the obligate intracellular bacterium Lawsonia ....
    BBSRC
    2005 - 2009
     
  • Secretomes of bacterial foodborne pathogens and functional assessment during interaction with intestinal epithelium
    BBSRC
    2005 - 2008