Institute of Cardiovascular and Medical Sciences

Dr Christopher Loughrey

Dr Christopher Loughrey
  • Senior Lecturer (Institute of Cardiovascular and Medical Sciences)
  • Associate Academic (School of Veterinary Medicine)

telephone: 01413302753
email: Christopher.Loughrey@glasgow.ac.uk


Dr. Christopher Loughrey is a veterinary surgeon and principal investigator within the Glasgow Cardiovascular Research Centre. His laboratory utilises a wide range of experimental techniques to investigate heart function at the level of the molecule, cell, organ and whole animal. This integrative approach provides the opportunity to study abnormal alterations of gene and protein expression/function and in turn how these changes influence whole heart function during cardiac disease. Dr. Loughrey’s laboratory has practical expertise in a number of methodologies including: confocal microscopy, single cell electrophysiology, fluorescence measurements/imaging, Western blotting, PCR, whole heart techniques (Langendorff perfusion and working heart preparations) and in vivo cardiovascular measurements/micro-surgery (e.g. intra-ventricular pressure-volume measurements, electrocardiography and a mouse model of myocardial infarction). These techniques are used in a number of projects which are on-going within the laboratory which aim to inform the development of therapeutic strategies to treat cardiac disease in animals and humans.

Research:

Member:

Jump to: 2013 | 2012 | 2011 | 2010 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002
Number of items: 19.

2013

Loughrey, C.M., and Gray, G.A. (2013) Advancing our understanding of the pathophysiology of cardiac disease using in vivo assessment of heart structure and function in rodent models. Experimental Physiology, 98 (3). pp. 599-600. ISSN 0958-0670 (doi:10.1113/expphysiol.2012.064550)

2012

Elliott, E.B., Kelly, A., Smith, G.L., and Loughrey, C.M. (2012) Isolated rabbit working heart function during progressive inhibition of myocardial SERCA activity. Circulation Research, 110 . pp. 1618-1627. ISSN 0009-7330 (doi:10.1161/CIRCRESAHA.111.262337)

Kelly, A., Elliott, E.B., Matsuda, R., Kaneko, N., Smith, G.L., and Loughrey, C.M. (2012) The effect of K201 on isolated working rabbit heart mechanical function during pharmacologically-induced Ca2+ overload. British Journal of Pharmacology, 165 (4b). pp. 1068-1083. ISSN 0007-1188 (doi:10.1111/j.1476-5381.2011.01531.x)

2011

Currie, S., Elliott, E.B., Smith, G.L., and Loughrey, C.M. (2011) Two candidates at the heart of dysfunction: The ryanodine receptor and calcium/calmodulin protein kinase II as potential targets for therapeutic intervention—An in vivo perspective. Pharmacology and Therapeutics, 131 (2). pp. 204-220. ISSN 0163-7258 (doi:10.1016/j.pharmthera.2011.02.006)

Elliott, E.B., Hasumi, H., Otani, N., Matsuda, T., Matsuda, R., Kaneko, N., Smith, G.L., and Loughrey, C.M. (2011) K201 (JTV-519) alters the spatiotemporal properties of diastolic Ca2+ release and the associated diastolic contraction during β-adrenergic stimulation in rat ventricular cardiomyocytes. Basic Research in Cardiology, 106 (6). pp. 1009-1022. ISSN 0300-8428 (doi:10.1007/s00395-011-0218-4)

2010

Toischer, K. et al. (2010) K201 improves aspects of the contractile performance of human failing myocardium via reduction in Ca2+ leak from the sarcoplasmic reticulum. Basic Research in Cardiology, 105 (2). pp. 279-287. ISSN 0300-8428 (doi:10.1007/s00395-009-0057-8)

2007

Loughrey, CM, Otani, N, Seidler, T, Craig, MA, Matsuda, R, Kaneko, N, and Smith, GL (2007) K201 modulates excitation-contraction coupling and spontaneous Ca2+ release in normal adult rabbit ventricular cardiomyocytes. Cardiovascular Research, 76 (2). pp. 236-246. ISSN 0008-6363 (doi:10.1016/j.cardiores.2007.06.014)

Seidler, T, Loughrey, CM, Zibrova, D, Kettlewell, S, Teucher, N, Kogler, H, Hasenfuss, G, and Smith, GL (2007) Overexpression of FK-506-binding protein 12.0 modulates excitation-contraction coupling in adult rabbit ventricular cardiomyocytes. Circulation Research, 101 (10). pp. 1020-1029. ISSN 0009-7330 (doi:10.1161/CIRCRESAHA.107.154609)

Volkers, M et al. (2007) S100A1 decreases calcium spark frequency and alters their spatial characteristics in permeabilized adult ventricular cardiomyocytes. Cell Calcium, 41 (2). pp. 135-143. ISSN 0143-4160 (doi:10.1016/j.ceca.2006.06.001)

2006

Colotti, G, Zamparelli, C, Verzili, D, Mella, M, Loughrey, CM, Smith, GL, and Chiancone, E (2006) The W105G and W99G sorcin mutants demonstrate the role of the D helix in the Ca2+-dependent interaction with annexin VII and the cardiac ryanodine receptor. Biochemistry, 45 (41). pp. 12519-12529. ISSN 0006-2960 (doi:10.1021/bi060416a)

2005

Miller, SLW, Currie, S, Loughrey, CM, Kettlewell, S, Seidler, T, Reynolds, DF, Hasenfuss, G, and Smith, GL (2005) Effects of calsequestrin over-expression on excitation-contraction coupling in isolated rabbit cardiomyocytes. Cardiovascular Research, 67 (4). pp. 667-677. ISSN 0008-6363 (doi:10.1016/j.cardiores.2005.04.023)

2004

Loughrey, C.M., Seidler, T., Miller, S.L.W., Prestle, J., MacEachern, K.E., Reynolds, D.F., Hasenfuss, G., and Smith, G.L. (2004) Over-expression of FK506-binding protein FKBP12.6 alters excitation-contraction coupling in adult rabbit cardiomyocytes. Journal of Physiology, 556 (3). pp. 919-934. ISSN 0022-3751 (doi:10.1113/jphysiol.2003.057166)

Loughrey, C.M., Smith, G.L., and MacEachern, K.E. (2004) Comparison of Ca2+ release and uptake characteristics of the sarcoplasmic reticulum in isolated horse and rabbit cardiomyocytes. American Journal of Physiology: Heart and Circulatory Physiology, 287 (3). H1149-H1159. ISSN 0363-6135 (doi:10.1152/ajpheart.00060.2004)

Currie, S, Loughrey, CM, Craig, MA, and Smith, GL (2004) Calcium/calmodulin-dependent protein kinase II delta associates with the ryanodine receptor complex and regulates channel function in rabbit heart. Biochemical Journal, 377 (2). pp. 357-366. ISSN 0264-6021 (doi:10.1042/BJ200310431)

Wokosin, DL, Loughrey, CM, and Smith, GL (2004) Characterization of a range of fura dyes with two-photon excitation. Biophysical Journal, 86 (3). pp. 1726-1738. ISSN 0006-3495 (doi:10.1016/S0006-3495(04)74241-1 )

2003

Loughrey, C.M., MacEachern, K.E., Cooper, J., and Smith, G.L. (2003) Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics. Cell Calcium, 34 (1). pp. 1-9. ISSN 0143-4160 (doi:10.1016/S0143-4160(03)00012-5)

Seidler, T et al. (2003) Effects of adenovirus-mediated sorcin overexpression on excitation-contraction coupling in isolated rabbit cardiomyocytes. Circulation Research, 93 (2). pp. 132-139. ISSN 0009-7330 (doi:10.1161/01.RES.0000081596.90205.E2)

Zhang, H. et al. (2003) Dynamics of cardiac intracellular Ca2+ handling - from experiments to virtual cells. International Journal of Bifurcation and Chaos, 13 (12). pp. 3535-3560. ISSN 0218-1274 (doi:10.1142/S0218127403008843)

2002

Loughrey, C.M., MacEachern, K.E., Neary, P., and Smith, G.L. (2002) The relationship between intracellular [Ca2+] and Ca2+ wave characteristics in permeabilised cardiomyocytes from the rabbit. Journal of Physiology, 543 (3). pp. 859-870. ISSN 0022-3751 (doi:10.1113/jphysiol.2002.021519)

This list was generated on Thu May 2 10:59:44 2013 BST.

Grants and Awards listed are those received whilst working with the University of Glasgow.

  • Investigating the therapeutic potential of cathepsin-L inhibition to limit ischaemia-reperfusion injury in the heart
    Scottish Executive Health Department
    2013 - 2015
     
  • Investigating the potential of cathepsin-L as a common therapeutic target and biomarker for coronary heart disease and African trypanosomiasis (ISSF Catalyst Fund)
    Wellcome Trust
    2011 - 2014
     
  • Angiotensin 1-9 and angiotensin 1-7: assessment of their mechanisms of action as counter-regulatory renin angiotensin system peptides in cardiovascular disease
    British Heart Foundation
    2011 - 2014
     
  • Investigating the expression and function of RUNX1 in cardiac tissue during myocardial infarction
    British Heart Foundation
    2009 - 2011
     
  • The role of direct parasite-cardiomyocyte interaction in the pathogenesis of the cardiac dysfunction observed in mammals with African trypanosomiasis
    Royal College of Veterinary Surgeons
    2008 - 2009
     
  • Targeted disruption of sarcoplasmic reticulum mediated Ca2+ handling and diastolic dysfunction: A comaprison between isolated cardiomyocytes and the whole heart.
    Heart Research UK
    2007 - 2009
     
  • Annual conference of the Biophysical Society 2007
    Royal Society
    2007 - 2007
     
  • Royal Society of Edinburgh international exchange programme (open) - veterinary medicine
    Royal Society of Edinburgh
    2006 - 2007
     
  • Investigating the role of intracellular calcium in the left ventricular diastolic dysfunction
    Medical Research Scotland
    2006 - 2008
     
  • Cellular Basis of Arrhythmias:The Inter-Relationship between Ca2+ Transients and Ca2+ Waves in Cardiac Muscle
    British Heart Foundation
    2006 - 2009
     
  • Use of Micro-Conductance Technology to investigate the link between altered sarcoplasmic reticulum calcium dynamics & diastolic dysfunction.
    Tenovus-Scotland
    2005 - 2006
     
  • Use of micro-conductance technology to study cardiac pressure-volume (PV) relations in ex vivo whole rabbit hearts during acute application.
    Royal Society
    2005 - 2006
     

Grant Advisory Board

  • 2010 - 2010: Wellcome Trust - International Mouse Phenotyping Project Advisory Meeting

Invited International Presentations

  • 2009: Aetas Pharma Ltd - Meeting
  • 2008: USA - Gordon Research Conference - Co-chair of conference symposium
  • 2008: Trondheim, Norway - Conference at Trondheim University
  • 2007: Aetas Pharma Ltd - Meeting
  • 2003: British Cardiac Society (UK) - Conference

Prizes, Awards and Distinctions

  • 2005: 'Annual Presentations by Britain's Top Young Scientists'
  • 2003: British Cardiac Society - Judge's Prize for Oral Presentation
  • 2001: Association of Veterinary Teachers and Research Workers (AVTRW) - President's Prize for Oral Presentation
  • 1997: Silver Medal by the Royal Agricultural Society - BVMS Degree
  • 1997: Eukanuba Nutrition Prize - BVMS Degree

Professional Learned Society

  • 2005 - ongoing: European Society of Cardiology (Cardiac Cellular Electrophysiology) - Member
  • 2005 - ongoing: Veterinary Cardiology Society - Member
  • 2002 - ongoing: Biophysical Society - USA - Member
  • 2002 - ongoing: British Cardiac Society - Member
  • 2002 - ongoing: International Society for Heart Research - Member
  • 2001 - ongoing: Physiological Society - Member
  • 2000 - ongoing: British Veterinary Association - Member
  • 2000 - ongoing: Royal College of Veterinary Surgeons - Member