UNIVERSITY of GLASGOW

RASOR
Part of the Faculty of Biomedical & Life Sciences
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RASOR

RASOR (Radical Solutions for Researching the proteome) is a unique research programme to advance proteomics and its applicaton in life sciences and biomedical reseach.  Drawing on interdisciplinary and complementary skills, the aim is to design new proteomics methods and equipment that will solve cutting edge research challenges.

The RASOR research programme is underpinned by the Doctoral Training Centre in Proteomic and Cell Technologies which trains an average of 10 students per year on a 4 year MRes/PhD programme in scientific areas interleaving with RASOR objectives, in the fields of proteomics, imaging and microfluidics, with a particular focus on a wide range of technologies in cell and molecular biology, microarrays, sensors, instrumentation and spectroscopy.

 

Rasor is funded through the Interdisciplinary Research Collaboration Initiative

new_bbsrc_colour 200x50     EPSRC 200x70   SFC 200x70



News & Events Headlines



RASOR Outreach - A Celebration of Achievement

We hosted a meeting at the Royal Society of Edinburgh to highlight some of the successful research output from the RCUK funded RASOR consortium on Wednesday 9 November 2011

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RASOR Final Meeting 2011

The RASOR Final Meeting took place on Thursday 13th October 2011 at Oran Mor, 731-735 Great Western Road, Glasgow, G12 8QX

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Recent Publications


  • Akagi,J., Khoshmanesh, K., Evan, B., Hall., Crosier, C., Cooper, J.M., Crosier, P. and Wlodkowic, D. (2012) Miniaturized Embryo Array for Automated Trapping, Immobilization and Microperfusion of Zebrafish Embryos. PLOS-ONE (Accepted)

  • Clark, A. & Cooper, J.M. (2012) Plasmon Shaping using Protein Nanoarrays and Molecular Lithography to Engineer Structural Color. Angewandte Chemie. (in Press)

  • Faley, SL, Coopland M, Reboud, J and Cooper, JM (2011) Intracellular protein trafficking kinetics in chronic myeloid leukemia stem cells using a microfluidic platform, Integrative Biology (in press)

  • Khoshmanesh, K., J., Akagia, J., Hallc, C.J., Crosierc, K.E., Crosierc, P.S., Cooper, J.M. and Wlodkowic, D. (2012) Mechanistic Rationale for Large Metazoan Embryo Manipulations on Chip-Based Devices, Biomicrofluidics (in Press).

  • Latham, T.; Mackay, L.; Karim, M.; Sproul, D.; Culley, J.; Harrison, D.; Hayward, L.; Langridge-Smith, P.; Gilbert, N.; Ramsahoye, B. (2012) Lactate, a product of glycolytic metabolism, inhibits histone deacetylase activity and promotes changes in gene expression. Nucleic Acids Research (in Press).

  • Lowther, J., Beattie, A. E., Langridge-Smith, P. R. R., Clarke, D. J., Campopiano, D. J. (2012) L-penicillamine is a mechanism-based inhibitor of serine palmitoyltransferase by forming a pyridoxal 5’-phosphate-thiazolidine adduct. Medicinal Chemistry Communications (in Press)

  • Mukherjee, A., Bilton, P. R., Mackay, L., Janoschka, A., Zhu, H., Rea, D., Langridge-Smith, P. R. R., Campopiano, D. J., Teschner, T., Trautwein, A. X., Schünemann, V., Sadler, P. J. (2012). Ferric ion hydr(oxo) clusters in the 'Venus-Fly-Trap' cleft of FbpA: Mössbauer, calorimetric and mass spectrometric studies. Journal of Biological Inorganic Chemistry (in Press).

  • Reboud, J., Wilson, R., Bourquin, Y., Zhang, Y. and Cooper, J.M. (2012) Nebulisation on a Disposable Array Structured with Phononic Lattices. Lab-on-a-Chip (in Press)

  • Scotcher, J., Clarke, D.J., Langridge-Smith, P.R.R. (2012) An affinity purification procedure to isolate oxidized p53. Analytical Biochemistry 420, 96-98.

  • Zarowna-Dabrowska, A., McKenna, E.O., Schutte, M.E., Glidle, A., Chen, L., Cuestas-Ayllon, C., Marshall, D., Pitt, A., Dawson, M.D., Gu, E., Cooper, J.M., Yin, H.B. (2012) Generation of primary hepatocyte microarrays by piezoelectric printing. Colloid Surf. B-Biointerfaces 89, 126-132.

  • Alghane, M., Chen, B. X., Fu, Y. Q., Li, Y., Luo, J. K., and Walton, A. J. (2011) Experimental and numerical investigation of acoustic streaming excited by using a surface acoustic wave device on a 128 degrees YX-LiNbO3 substrate, J. Micromech. Microeng. 21.

  • Al-Mulla, F., Bitar, M. S., Al-Maghrebi, M., Behbehani, A. I., Al-Ali, W., Rath, O., Doyle, B., Tan, K. Y., Pitt, A., and Kolch, W. (2011) Raf Kinase Inhibitor Protein RKIP Enhances Signaling by Glycogen Synthase Kinase-3 beta, Cancer Res. 71, 1334-1343.

  • Bourquin, Y., Reboud, J., Wilson, R., Zhang, Y., and Cooper, J. M. (2011) Integrated immunoassay using tuneable surface acoustic waves and lensfree detection, Lab Chip 11, 2725-2730.

  • Bourquin, Y. Wilson, R., Reboud, J., Zhang, Y. and Cooper, J.M. (2011) Phononic Crystals for Shaping Fluids. Advanced Materials, 23 (12) 1458-1462

  • Clark, A. W., and Cooper, J. M. (2011) Nanogap Ring Antennae as Plasmonically Coupled SERRS Substrates, Small 7, 119-125.

  • Clarke, D., Murray, E., Hupp, T., Mackay, C.L., Langridge-Smith, P.  (2011). "Mapping a Noncovalent Protein–Peptide Interface by Top-Down FTICR Mass Spectrometry Using Electron Capture Dissociation." Journal of the American Society for Mass Spectrometry (Epub ahead of print).

  • Dastidar, S. G., Raghunathan, D., Nicholson, J., Hupp, T. R., Lane, D. P., and Verma, C. S. (2011) Chemical states of the N-terminal "lid" of MDM2 regulate p53 binding Simulations reveal complexities of modulation, Cell Cycle 10, 82-89.

  • Faley, S.L., Reboud, J., Copland, M. & Cooper, J.M. (2011) Cell Chip Array for Microfluidic Proteomics enabling Rapid in situ Assessment of Intracellular Protein Phosphorylation. Biomicrofluidics, in press

  • Goodwin, R. J. A., Pitt, A. R., Harrison, D., Weidt, S. K., Langridge-Smith, P. R. R., Barrett, M. P., Mackay, C. L. (2011). Matrix-free mass spectrometric imaging using laser desorption ionisation Fourier transform ion cyclotron resonance mass spectrometry. Rapid Communications in Mass Spectrometry 25(7): 969-972.

  • Graham, D., Stevenson, R., Thompson, D. G., Barrett, L., Dalton, C., and Faulds, K. (2011) Combining functionalised nanoparticles and SERS for the detection of DNA relating to disease, Faraday Discuss. 149, 291-299.

  • Gruber, D., Li, Y., Smith, S., Tiwari, A., Stokes, A.A., Terry, J.G., MacKay, L., Langridge-Smith, P., Walton, A.J. (2011) Test structures and a measurement system for characterising the lifetime of EWOD devices, IEEE International Conference on Microelectronics Test Structures, pp. 80-84, 2011.

  • Khoshmanesh, K., Akagi, J., Nahavandi, S., Skommer, J., Baratchi, S., Cooper, J. M., Kalantar-Zadeh, K., Williams, D. E., and Wlodkowic, D. (2011) Dynamic Analysis of Drug-Induced Cytotoxicity Using Chip-Based Dielectrophoretic Cell Immobilization Technology, Anal. Chem. 83, 2133-2144.

  • Khoshmanesh, K., Akagi, J. Nahavand, S., Skommer, J., Baratchi, S., Cooper, J.M., Kalantar, K., Williams, D., Wlodkowic, D. (2011) Dynamic Analysis of Drug-Induced Cytotoxicity using Chip-Based Dielectrophoretic Cell Immobilisation Technology. Analytical Chemistry, 83 (6): 2133-2144

  • Khoshmanesh, K., J., Nahavand, S., Kalantar, K.,  Baratchi, S., Williams, D., Cooper, J.M., Wlodkowic, D. (2011) Interfacing Cell Based Assays in ESEM Using Dielectrophoresis. Analytical Chemistry, 83 (8): 3217-3221.

  • Khoshmanesh, K., J., Nahavand, S., Kalantar, K.,  Baratchi, S., Williams, D., Cooper, J.M., Wlodkowic, D. (2011) Trapping and Imaging of Micron-sized Embryos using Dielectrophoresis,Electrophoresis, (in press).

  • Li, Y., McKenna, E. O., Parkes, W., Pitt, A. R. and Walton, A. J. (2011) The application of fixed hydrophobic patterns for confinement of aqueous solutions in proteomic microarrays. Applied Physics Letters. (Accepted).

  • Martino, C., Zagnoni, M., Sandison, M., Chanasakulniyom, M., Pitt, A. and Cooper, J.M. (2011) Intracellular Protein Determination using Droplet-Based Immunoassays. Analytical Chemistry, May 16, (Epub ahead of print).

  • Ono, M., Scott, M. S., Yamada, K., Avolio, F., Barton, G. J., and Lamond, A. I. (2011) Identification of human miRNA precursors that resemble box C/D snoRNAs, Nucleic Acids Res. 39, 3879-3891.

  • Redelinghuys P, Antonopoulos A, Liu Y, Campanero-Rhodes MA, McKenzie E, Haslam SM, Dell A, Feizi T, Crocker PR. (2011) Early murine T-lymphocyte activation is accompanied by a switch from N-Glycolyl- to N-acetyl-neuraminic acid and generation of ligands for siglec-E. J Biol Chem. 286(40):34522-32.
  • Scotcher, J., Clarke, D., Weidt, S., Mackay, C.L., Hupp, T., Sadler, P., Langridge-Smith, P.  (2011). "Identification of Two Reactive Cysteine Residues in the Tumor Suppressor Protein p53 Using Top-Down FTICR Mass Spectrometry." Journal of the American Society for Mass Spectrometry 22(5): 888-897.

  • Thompson, D. G., McKenna, E. O., Pitt, A. and Graham, D. (2011) Microscale mesoarrays created by dip-pen nanolithography for screening of protein –protein interactions, Biosensors and Bioelectronics, 26, 4667-4673.

  • Wlodkowic, D. and Cooper, J.M. (2011) Wormometry-on-a-Chip: Innovative Technologies for in situ Analysis of Small Multicellular  Organisms. Accepted Cytometry, Part A. 4 May 2011 (Epub ahead of print).

  • Wlodkowic, D.  and Cooper, J.M. Microfluidics: New Prospects for Multidimensional Studies of Tumour Cell Death. Accepted, A-Articles (2011)

  • Wlodkowic, D., Khoshmanesh, K., Sharpe, J., Darzynkiewicz, Z. and Cooper, JM. (2011) Apoptosis goes on a chip: advances in the microfluidic analysis of programmed cell death. Accepted Analytical Chemistry

  • Wilson, R., Reboud, J., Bourquin, Y., Neale, S. L., Zhang, Y., and Cooper, J. M. (2011) Phononic crystal structures for acoustically driven microfluidic manipulations, Lab Chip 11, 323-328.