Next Generation Sequencing Illumina Service

Next Generation Sequencing (NGS), which is a massively parallel sequencing of huge number of short, immobilized and amplified DNA templates, has delivered a mean to sequence DNA at unprecedented speed and very low cost. We can now sequence entire genomes or transcriptoms in a matter of days or weeks. The NGS service at SHWFGF using Illumina Genome Analyser IIx is now fully operational. Please, contact us for discussing the experiment design, sample preparation, timing, price and data analysis issue.

Sequencing modes

• Single ended (SE) - DNA templates are sequenced from one end only
  
• Pair ended (PE) -  DNA templates are sequenced from both ends)
  
• 
sample multiplexing - up to 12 samples can be bar-coded and put on one lane
  

Current performance

• 76 long reads run routinely 
  
• 25-30 million reads from one lane in SE mode and twice as much in PE mode
  
• reads qualities high and stable - better than Q25-30 for the first 70bp
  

Applications

• gDNA-seq - sequencing of genomic DNA
  
• ChIP-seq - sequencing DNA obtained from chromatin immunoprecipitation experiments
  
• mRNA-seq - sequencing of the entire transcriptome
  
• sRNA-seq - sequencing of small RNA content

  

Data analysis: Two full time bioinformaticians deliver bioinformatics support for projects

• run at SHWFGF
  
• external projects via SULSA Transcriptomics Facility
  
• external projects run in MRC Sequencing Hub in GenePool (Edinburgh)